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Purification Related Discussions
  1. 230nm peak with NanoDrop - after gel purification (reply: 2)
  2. Single colony purification by streaking - (reply: 4)
  3. GST protein purification - (reply: 1)
  4. storage of purified proteins? - (reply: 1)
  5. His-tag purified protein is yellow! - (reply: 7)
  6. general protein purification from mouse tissue - (reply: 4)
  7. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
  8. Purification of gst and His tagged protein - after purification there are still many contaminated bands (reply: 1)
  9. superscript indirect cDNA labeling system - superscriptIII, cDNA purification, microarray, free amines (reply: 3)
  10. How to reuse DNA purification columns? - (reply: 3)
  11. native purification of His-tagged proteins from E. coli? - (reply: 1)
  12. protein purification - (reply: 4)
  13. Purification of RF DNA from phage prep - Need to get rid of ssDNA contamination (reply: 2)
  14. Troubleshooting protein purification cation IEX - Cation IEX (reply: 1)
  15. qiagen dna purification columns - are they all the same? (reply: 2)
  16. DNA purification after sonication - (reply: 12)
  17. QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
  18. purifying small DNA fragmets - (reply: 2)
  19. Protein purification - (reply: 5)
  20. Purified cDNA Clonning - (reply: 3)
  21. polysaccharide purification - (reply: 13)
  22. Purification granulocyte factor - (reply: 2)
  23. Which quantity of purified protein in WB? - (reply: 2)
  24. mRNA purification - (reply: 2)
  25. Western Blot with HPLC purified samples - non specific binding??? need help desperately (reply: 1)
  26. Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
  27. protein purification from yeast - (reply: 2)
  28. Purification Optimisation - (reply: 4)
  29. Dna purification from gel - (reply: 20)
  30. Protocol for IgG Purification from Ascites? - (reply: 1)
  31. sequential PCR and purification before TA cloning - (reply: 1)
  32. Problems with purifying a secret His-protein expressed in plant - (reply: 3)
  33. Problem with His Tag purification - using Promega HisLink spin purification kit (reply: 1)
  34. do i need to desalt my protein after purification - (reply: 1)
  35. Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
  36. Purifying protein with no his tag - (reply: 5)
  37. Purified PCR product - (reply: 2)
  38. how to refold insoluble protein after purification - (reply: 10)
  39. Collagen IV purification - (reply: 2)
  40. DNA gel purification without a spin column - (reply: 4)
  41. Peptide purification - (reply: 1)
  42. storage buffer for purified protein - (reply: 3)
  43. Gel or column purify - (reply: 4)
  44. Big problem in GST Protein purification - (reply: 9)
  45. protein purification - (reply: 5)
  46. IgG purification: Multimerization... - (reply: 4)
  47. Solutions for manual RNA purification - (reply: 2)
  48. His tagged protein purification - (reply: 2)
  49. storage of ammonium sulphate purified antibodies - (reply: 1)
  50. How to purify 700bp pcr product? - (reply: 5)
  51. Gel purification: SpinX vs commercial kits - Gel purification (reply: 3)
  52. no aminoallyl-dye conjugation after purification - (reply: 3)
  53. the problem in gel purification - (reply: 4)
  54. PCR purification /DMSO / vanishing DNA - (reply: 2)
  55. mRNA purification, stratagene - principles of mRNA oligo(dT) magnetic particles (reply: 3)
  56. simultaneos DNA purification - (reply: 5)
  57. Remove albumin from purified protein sample? Help please - (reply: 5)
  58. Couple peptide to beads? for antibody purification - (reply: 1)
  59. Methods for large-scale protein purification - (reply: 5)
  60. affinity purification of antibodies - what is the best buffer to elute (reply: 2)
  61. pcr product purification - (reply: 7)
  62. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
  63. how to purify ChIP DNA by using kit - (reply: 4)
  64. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
  65. Purification of HisTag Recombinant Protein - Advices needed (reply: 2)
  66. Affinity purification of antibody - (reply: 2)
  67. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
  68. Protein purification with HPLC - (reply: 6)
  69. GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
  70. Gel purification of plasmid after each of these steps? - (reply: 1)
  71. Sodium Citrate in DNA purification - (reply: 4)
  72. DNA purification technique - After a PCR (reply: 8)
  73. RNA purification from pancreas - troubles with the pancreas (reply: 1)
  74. double tag purification. - (reply: 1)
  75. Low concentration after gel purification - PCR product purification (reply: 6)
  76. gst purification, got extra band - (reply: 4)
  77. Gel purification of DNA bands-any alternates? - (reply: 4)
  78. unsuccessful gel purification - (reply: 8)
  79. How can I purify the wanted protein - (reply: 10)
  80. HA-protein purification - (reply: 1)
  81. Question for purchasing purified protein - (reply: 3)
  82. Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
  83. purification of proteins from sample - (reply: 12)
  84. dialysis tubes for chromatin purification - (reply: 5)
  85. His tag protein binding to resin ... - can i purify off the resin? (reply: 5)
  86. How long can U store DNA Sample - DNA Purification (reply: 3)
  87. purification prblm - big blunder (reply: 4)
  88. GST and His tagged proteins - Does the GST give problems in His tag purification? (reply: 5)
  89. serum purification? - (reply: 6)
  90. Protein purification - (reply: 3)
  91. How many of us request for PAGE purification of primers more than 30 mers in len - PAGE purified primers (reply: 5)
  92. CsCl density for BAC purification - (reply: 1)
  93. DNA ligation - problem with purification (reply: 4)
  94. Purification of GST-fusion - GST-fusion protein: How to express and purify (reply: 7)
  95. how to purify plasmid and genomic dna - (reply: 3)
  96. Specific and Total Activity for Protein Purification - (reply: 2)
  97. Protein purification after cleavage (thioredoxin) - (reply: 3)
  98. Epithelium cell purification - (reply: 2)
  99. large protein degradation - during baculovirus purification (reply: 2)
  100. on-collumn DNASe in RNA purification - (reply: 3)
  101. will purification processes lead to loss of RNA? - (reply: 1)
  102. ha-tag purification , lots of bands - (reply: 4)
  103. Purification and cloing of large fragments - cloning (reply: 3)
  104. Purification after oligos phosphorylation? - (reply: 1)
  105. visualizing purified protein - (reply: 9)
  106. Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
  107. antibody purification - how to purify from serum free medium (reply: 1)
  108. in vitro translation - how is the protein purified? (reply: 12)
  109. Purification of protein from plasma - How to separate a 30kDa protein from serum albumin (reply: 2)
  110. plasmid purification from mammalian cells? - (reply: 1)
  111. GST purification multiple band problem - GST purification problem, multiple band (reply: 3)
  112. Protein purification before mass-spec - need sample purification methods (reply: 4)
  113. what these components do in the buffer used in protein purification - (reply: 5)
  114. his-tag thrombin cleavage in-column? - as a purification strategy (reply: 1)
  115. Gel purification of PCR products - (reply: 2)
  116. Purification kits - Free samples (reply: 8)
  117. Purify lambda dsDNA bound to dye from unbound dye - (reply: 1)
  118. phenol/CHCl3 vs purification from gel - (reply: 1)
  119. dna purification..help - plz..help (reply: 2)
  120. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
  121. Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
  122. [WTA] Phyto-pathogenic Protocol. - how to carry out isolation, extraction and purification of phytopathogen from pl (reply: 1)
  123. dna purification - (reply: 6)
  124. How do you purify membrane protein? - help (reply: 2)
  125. gel purification of PCR product - (reply: 1)
  126. trouble getting cysteine labeled with biotin-maleimide - cysteine scanning purified protein (reply: 1)
  127. Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
  128. Question about the strategy of protein purification - (reply: 4)
  129. RNA extraction and purification - (reply: 6)
  130. How to get rid of contaminated nucleases from purified proteins - (reply: 2)
  131. Asking help for purifying plasmids - (reply: 3)
  132. Protein Purification - (reply: 1)
  133. Should I purify digested vector before ligation? - (reply: 7)
  134. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  135. Protein Purification - Problem in protein purification (reply: 6)
  136. NI-NTA colunm purification - no bands after purification (reply: 2)
  137. buffer for His-tag recombinant protein purification - (reply: 2)
  138. Problems in DNA sequencing after gel purification - (reply: 9)
  139. nested PCR: purification? - (reply: 1)
  140. purification failed....please tell me what to do.... - (reply: 6)
  141. DNA purification for transfection - someone have a protocol??? (reply: 1)
  142. Protein purification problem - protein purification problem (reply: 1)
  143. Affinity purification question.....do not want to couple with NH2 groups - (reply: 2)
  144. Cell line to be used for protein expression an purification - (reply: 8)
  145. His tag protein purification problem - Binding of undesired protein (reply: 6)
  146. Anion exchanger chromatography for virus purification - (reply: 1)
  147. Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
  148. Genomic DNA purification protocol - (reply: 6)
  149. shRNA insert purification - (reply: 4)
  150. siRNA purification - (reply: 2)
  151. a problem of genomic DNA purification - (reply: 3)
  152. protein complex purification, what would affect? - (reply: 3)
  153. two step purification (SPA) concern change to one step? - (reply: 5)
  154. Gst tag purification problem - (reply: 5)
  155. Protein purification wash problem - (reply: 1)
  156. adenovirus purification - (reply: 1)
  157. problem with bacteriocin protein purification - (reply: 2)
  158. at which step can I store the protein? - purification from inclusion body... (reply: 3)
  159. purification problem. - most of my protein is washed away.... (reply: 10)
  160. Help me! No colonies! Problem with gel purification? - (reply: 5)
  161. Purification of His-tagget protein from inclusion bodies - (reply: 12)
  162. suggest protein purification methods? - (reply: 2)
  163. Please recommend a fast way to get purified protein - (reply: 2)
  164. Plasmid DNA purification - (reply: 3)
  165. protein purification - (reply: 1)
  166. Purify integrating PCR product before transforming yeast? - (reply: 1)
  167. Concentrating recombinent antagens - hisTaged antagens purified and neen to concentrated (reply: 1)
  168. purification columns for ssDNA - (reply: 1)
  169. synaptosome purification - (reply: 1)
  170. Gel shift assay for non purified protein preps - (reply: 1)
  171. Size exclusion spin columns for oligo purification ... - (reply: 1)
  172. Plasmid purification - (reply: 5)
  173. phenol/chloroform of purified PCR product after RE digestion - what are we trying to purify? (reply: 4)
  174. Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
  175. purification of prot interacting with a probe in EMSA - (reply: 8)
  176. Type I collagen - extraction and purification - (reply: 1)
  177. Porblem using streptavidin coated beads - Using streptavidin coated beads to purify proteins tagged with a biotinylated ol (reply: 2)
  178. Role of buffer in antibody purification - (reply: 3)
  179. magic? - got more product after PCR purification... (reply: 3)
  180. purification for GST pull down - (reply: 1)
  181. Contamination after purification - (reply: 1)
  182. PCR product purification - (reply: 3)
  183. lysis is not translucent after urea treatment - need help with His tag fusion protein purification (reply: 2)
  184. DNA Purification from gels - (reply: 4)
  185. Removing salt and immidazole from purified Protein - (reply: 2)
  186. Ni-NTA column purification - Ni-NTA column purification (reply: 1)
  187. why glycerol used in protein purification - (reply: 1)
  188. gel purification after restriction digestion - (reply: 3)
  189. Ab purification from supernatant - dialysis of large volumes (2L) (reply: 3)
  190. Best way to purify PCR product for sequencing - (reply: 2)
  191. anti-c-myc-agarose protein purification - (reply: 1)
  192. Can we purify Miniprep DNA and use for transfection? - (reply: 3)
  193. Aggregation of protein after purification? - (reply: 2)
  194. purify Ab precipitate after neutralize to pH7 - (reply: 2)
  195. DNA purification - (reply: 1)
  196. Purification of enzymatically active protein from PAGE - (reply: 9)
  197. SOS: Gel purification of small size DNA - (reply: 6)
  198. purification of Anti-p24 antibody - purification of Anti-p24 antibody (reply: 1)
  199. protein purification - (reply: 1)
  200. Help with purification of antibody against a GST-fusion - Antibody purification (reply: 2)
  201. Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
  202. High quality gDNA - need recommendation for purification method (reply: 1)
  203. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
  204. Odd curve of Gel purified PCR product - (reply: 5)
  205. PCR purification basics? - (reply: 6)
  206. Large scale purification of large size plasmid - (reply: 1)
  207. RNA purification from macrophages - (reply: 5)
  208. Oligo purification - (reply: 5)
  209. Proteins and marker are not running down gel - Protein purification (reply: 7)
  210. Does CHAPS interfere with GST purification?? - (reply: 1)
  211. Purification after digestion - do I need to do it? (reply: 1)
  212. dialysis of purified protein - (reply: 5)
  213. Using qiagen kit for ChIP DNA purification - (reply: 4)
  214. can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
  215. Purification of Genomic DNA - (reply: 2)
  216. Anybody happens to know how to purify RubisCO - (reply: 1)
  217. Unspecific bands after gel purification, why? - (reply: 7)
  218. batch purification - (reply: 1)
  219. His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
  220. problems purifying plasmid DNA after restriction digestion - (reply: 3)
  221. Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
  222. transfection-grade plasmid - plasmid extraction or purification kit for transfection (reply: 4)
  223. Purification of Double stranded radiolabelled DNA - Purification problems (reply: 3)
  224. separated two protein - protein purification (reply: 4)
  225. Promega Gel Purification Kit - Cant get rid of ethanol (reply: 3)
  226. A Question about purification in making clones - (reply: 2)
  227. Taq polymerase - Produce and Purify (reply: 3)
  228. Anybody knows any resolutions to improve TAP purification? - (reply: 1)
  229. DNA purification - (reply: 3)
  230. help for large-scale plasmid purification - (reply: 3)
  231. his tag purification - (reply: 1)
  232. TAP Tagged Affinity Purification + Sticking to IgG-seph - (reply: 5)
  233. purification of RNAP from E.coli - (reply: 2)
  234. problems with loss of purified protein due to precipitation - protein purification (reply: 4)
  235. Purification of nuclear proteins - (reply: 2)
  236. DNase treatment for RNA purification - (reply: 6)
  237. PCR product purification - (reply: 58)
  238. Problems purifying DGGE bands - PCR/DGGE (reply: 8)
  239. His purification of a regular expressed protein - (reply: 1)
  240. Purification of a cell membrane protein - (reply: 1)
  241. protein precipitates after dialysis - Protein purification (reply: 4)
  242. IgM Purification - (reply: 1)
  243. His-tag protein purification - (reply: 5)
  244. Ni affinity purification - not as simple as you may think - Protein purification (reply: 5)
  245. antigen purification - (reply: 1)
  246. His tag - protein purification - (reply: 2)
  247. Fibronectin Purification - (reply: 1)
  248. how to purify small fragment of DNA? - (reply: 2)
  249. Problems with PCR Purification - When I try to purify my PCR product I lose all of my DNA? (reply: 4)