Does CHAPS interfere with GST purification?? - (Jun/29/2005 )

Hi guys,

I've solubilized an unknown receptor using CHAPS as the main resuspension/storage buffer. Now I have the receptor's ligand (which is a recombinant GST fusion protein) immobilised onto agarose-GSH. My question is this: when I run my solubilized receptor through the column, will the CHAPS interfere with the affinity between the agarose-GSH and my recombinant ligand fusion protein?

Thanks

Don't worry... i think i found the answer but still feel free to post your comments.

this is my source btw:

"Interactions between Phage-Shock proteins in E. Coli"
Hendrik Adams, Wieke Teerstra, Jeroen Demmers, Rolf Boesten and Jan Tommassen
18 November 2002
pp1174-1180

Check out page 1176 in Materials and Methods under the title 'GST-PspA and GST-PspB pull-down assays'.


Dr Washo