Asking help for purifying plasmids - (Aug/08/2006 )
I received plasmids from another lab on filter paper. And I have put the filter paper in a tube and dissolved the plasmids in TE in a total volume of 30ul. Due to my careless manipulation, I added 25 ul competent E coli into the tube containing my plasmids. The plasmids are important for me.
Does any one know if the solution will be suitable to transform E.coli or how to purify my plasmids from the solution?
honestly, i would just try to transform with that solution you have.
adjust for volume, and have at it. electroporation, or chemical transformation?
yeah, I would try to heat or electroshock that mix you did by accident, or ….take I don't know..mm.. 10-15 ul to 50 or 100 ul competent cells to transform, It wouldn't be a problem!!
Do you mean that the whole mixture go through heating or electroshocking and then be plated on medium with antibiotics added?