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PMSF in AChE purification :unsure: - (Aug/29/2006 )

i hv to purify the AChE from fish brain.
is this possible i use PMSF as protien inhibitor??
what is the concept behind in using PMSF! unsure.gif

-heng1011-

PMSF is a protease inhibitor. It's almost impossible to keep your samples well cooled during protein isolation and during the isolation also proteases come free.
But I cannot help you with fish brain, but PMSF sounds normal.

-aspergillie-