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CsCl density for BAC purification - (Feb/19/2007 )


I tried to purify the megaplasmid of around 140kb to get rid of contamination of chromosomal DNA.Dose any one have a similar protocol to purify the plasmid by CsCl gradient method,similar to BAC (>100Kb) ,or Ti plasmid purification.What the CsCl density should I use?

Thank you very much.



there is a protocol for bacmid preparation here

see also here : isolating large plasmids, is it possible