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enzyme purification - (Apr/12/2006 )

Hi, I need to isolate an enzyme from C elegans. I have been searching in the net and in books and i really found many ways to do it. So i got a little confused. Is it there someone that had done this? I really need some advice about wich is the best method.
Thank you in advance


An enzyme?? Which one?


A lot of enzymes need their own procedure for cleaning. Steps like centrifugation, precipitation, different columns (SCX, gel filtration), pH, conductivity...

So, give some information about your protein!


[Sorry, it is Dicer the enzyme i want to purify...