tRNA expression and purification - (Jun/20/2006 )

Hi there,
Anybody with expertise in tRNA expression and purification from E. coli? Any protocol, detailed reference or suggestion will be highly appreciated.
Thanks in advance.
Bis

-Bis-

QUOTE (Bis @ Jun 20 2006, 10:51 PM)

Hi there,
Anybody with expertise in tRNA expression and purification from E. coli? Any protocol, detailed reference or suggestion will be highly appreciated.
Thanks in advance.
Bis

Why do you want to express/purify tRNA in E.coli? In vitro transcription using T7 Polymerase is a routine practice.

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