transfection-grade plasmid - plasmid extraction or purification kit for transfection (Feb/28/2005 )
hello, everyone, would you please recommend me a plasmid preparation kit for transfection? thanks a lot!
I use genelute from sigma. Not the cheapest but quick and simple. No messing about with ethanol/isopropanol precipitations at the end. Less steps to go wrong, reliable and good yield
I normally use Qiagen, or Macherey-Nagel which is the same but cheaper. I'm not getting good yields, though. Next time I'll be sure to try this Genelute thing.
We use Qiagen or Macherey-Nagel kit, too. but with good results. Qiagen Gigapreps really rule - IMHO.
THANKS all of your information very much!
I would like to try genelute.