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Fibronectin Purification - (Jun/28/2001 )


Could you give me some comment about Fibronectin Purification process?

After first elution using 4M urea, for second gelatin column, how can I eliminate urea?

dialysis or prewashing gelatin column with 4M urea is right?


dialysis will be fine, make sure you add a protease blocker in your dialysis buffer