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Western blotting Related Discussions
  1. Preventing contamination in TBS/T (WB wash buffer) - (reply: 5)
  2. Can anyone help me with Western blotting problem? - (reply: 6)
  3. western blotting troubleshooting - (reply: 3)
  4. Question on western blot picture - (reply: 2)
  5. how to interpret this WB result - (reply: 7)
  6. SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample - (reply: 1)
  7. coIP followed by a Western Blot - (reply: 4)
  8. WB imaging on Vilber-Lourmat Fusion - (reply: 1)
  9. Low weight protein degradation during western blot - (reply: 1)
  10. Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
  11. phospho-creb western blotting - (reply: 3)
  12. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
  13. Non-specific binding of protein in western blot - (reply: 3)
  14. 2D PAGE and Western blotting - (reply: 1)
  15. Lane spillover (?) on Western Blot - (reply: 3)
  16. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
  17. No band detected in WB - (reply: 4)
  18. Western Blot diffuse bands - (reply: 3)
  19. Human cell membrane protein Western blot! - (reply: 2)
  20. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
  21. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
  22. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
  23. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
  24. what should be used as controls during western blotting? - (reply: 4)
  25. Dumbbell-shaped bands on the WB - (reply: 3)
  26. Help in western blotting GAPDH bands are not even - (reply: 1)
  27. 37kb and 7kb protein in western blot - (reply: 24)
  28. Dot blot/Western background signal problem - (reply: 1)
  29. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
  30. What is best to measure protein expression: western blot or ELISA? - (reply: 4)
  31. Two bands in WB but one band in IP - (reply: 1)
  32. Two bands in WB but one band in IP - (reply: 2)
  33. Western blot detects exogenous protein but not endogenous - (reply: 2)
  34. White bands in western blotting - (reply: 4)
  35. How to obtain a control positive for my WB - (reply: 3)
  36. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  37. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  38. Question on loss of signal between samples in western blot - (reply: 4)
  39. Sample prep of cells for FACS analysis and running WB - (reply: 4)
  40. PCR & Western Blot sample preperation - (reply: 2)
  41. PCR & Western Blot sample preperation - (reply: 5)
  42. How to optimise my western semi-dry transfer? - (reply: 3)
  43. Help on native PAGE gel and its western blot result - (reply: 5)
  44. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  45. No protein on western blot but standard transferred - (reply: 2)
  46. Can't detect GAPDH (western) with Alexa488 secondary antibody - (reply: 6)
  47. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
  48. how to choose a proper tag for western blotting - (reply: 6)
  49. Strange band pattern in Western Blot - (reply: 1)
  50. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  51. RIPA buffer for IP and western - (reply: 1)
  52. Problems of western signal - (reply: 5)
  53. Western Blot Help - (reply: 1)
  54. Western Blot: Unequal Sample Migration - (reply: 4)
  55. Western blot with smeared bands - (reply: 3)
  56. Blocking agent in western blotting of histone proteins - (reply: 1)
  57. Best western loading control for comparing different cell lines - (reply: 2)
  58. western blotting running problem? - (reply: 2)
  59. Keeping apoptotic/detached cells for WB? - (reply: 3)
  60. Advice with WB please - (reply: 3)
  61. Western Blot Wet Transfer Conditions - (reply: 10)
  62. Western Blot and Inmunofluorescence contradictions - (reply: 12)
  63. Nanog double band in WB - (reply: 1)
  64. Help with western blot!! - (reply: 1)
  65. Band on Western Blot are smeared - (reply: 1)
  66. Can Anti-IgG Horseradish peroxidase be used in a whole blood western blot assay? - (reply: 1)
  67. advice in western blotting - (reply: 1)
  68. can antibodies recommended for western blot be used for immunopreipitation - (reply: 2)
  69. Please help uneven bands in western blot - (reply: 9)
  70. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
  71. Cell Lysate Western Blot Little or No Signal - (reply: 7)
  72. Need Help with Western blot - (reply: 1)
  73. Western Blot Problems...too many unspecific bands - (reply: 4)
  74. IL 8 - western blotting - (reply: 1)
  75. how to make fast blocking buffer in western blot? - (reply: 1)
  76. Wester blot analysis - (reply: 5)
  77. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  78. timecourse experiment for Western and qPCR - (reply: 2)
  79. Western blotting - (reply: 4)
  80. Fixing concentration for western blot from MTT - (reply: 1)
  81. Western Blotting of Amyloid Beta - (reply: 1)
  82. Need help translating my western blot protocol into every language - (reply: 3)
  83. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
  84. Western blot to CD44? - (reply: 3)
  85. Suggestion for anti CD44 antibody for WB and IP? - (reply: 1)
  86. Problems with western membrane stripping - (reply: 2)
  87. western blot of cardiomyocytes - (reply: 2)
  88. Western blot using blood plasma - (reply: 5)
  89. Smear formed in western blots while using tubulin as control - (reply: 4)
  90. How to confirm the band of interest in Westerns blot - (reply: 6)
  91. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  92. Western after ChIP - how to do that? - (reply: 2)
  93. Western not working - (reply: 4)
  94. fused bands of beta-actin in western blot - (reply: 2)
  95. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
  96. Problem with GFP detection in western - (reply: 6)
  97. Wrong protein size on western blot - (reply: 10)
  98. How to know if the signal of western blot is saturated ? - (reply: 4)
  99. Uneven banding - Western blot troubleshooting - (reply: 4)
  100. Western Blot with fixed cells - (reply: 5)
  101. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
  102. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
  103. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
  104. 3 extra bands show up on my western after an IP - (reply: 2)
  105. about western blot analysis - (reply: 1)
  106. Western Blotting loading control for large proteins - (reply: 2)
  107. Western Blotting of Amyloid Beta - (reply: 3)
  108. Western Blotting of LRRK2 - (reply: 1)
  109. Protein of 460 kD on western blot - (reply: 3)
  110. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
  111. NCOR1 western - (reply: 8)
  112. Discrepancy between WB and Flow cytometry result - (reply: 5)
  113. Western blotting transfer - (reply: 2)
  114. Western Quantification (No BCA???) - (reply: 2)
  115. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  116. Proper western blotting - (reply: 6)
  117. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
  118. No bands/weird bands on western blot - (reply: 3)
  119. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
  120. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
  121. please help with my western blots! - (reply: 3)
  122. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  123. Western blot- bands weak - (reply: 11)
  124. Preparing supernatant from cell culture for western blot - (reply: 4)
  125. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
  126. Curved bands in Western Blot - (reply: 2)
  127. Western blot and immunofluorescence staining - (reply: 9)
  128. Western blot and Triton X-100 in blocking buffer - (reply: 1)
  129. Dot blot translation to WB - (reply: 2)
  130. western blotting troubleshooting - (reply: 2)
  131. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  132. EPIGENETICS : Help required ... Histone Western Blot - (reply: 1)
  133. Far western blot: probe concentration - (reply: 1)
  134. Western blot question- High % Gel/Low MW Protein - (reply: 7)
  135. western blot explenation - (reply: 8)
  136. WB of cellular proteins in human serum - (reply: 2)
  137. Western blotting - (reply: 9)
  138. Phosphotyrosine western blotting, HELP! - (reply: 1)
  139. General chat about tween-20 as a WB block and wash - (reply: 1)
  140. western blot troubleshooting - (reply: 5)
  141. Membrane Protein SDS PAGE and WB - (reply: 1)
  142. western blot and actin issue - (reply: 2)
  143. Separating proteing with difference of 5Kd on western blot - (reply: 4)
  144. Immunoprecipitated protein visible on coomassie but not detected by Western - (reply: 4)
  145. Western Blot, Bax, Many bands - (reply: 3)
  146. Eliminating Protein/Protein Interactions for Western Blot - (reply: 2)
  147. Western Blot for Histone 3 K27me? - (reply: 1)
  148. Western Blot v ELISA for Different Samples? - (reply: 1)
  149. CCRK (CDK20) protein expression and western blot - (reply: 7)
  150. IGF2 antibody for immunohistochemistry/WB in mouse? - (reply: 2)
  151. SDS Page and WB: Wrong size bands - (reply: 2)
  152. Good Scanner for Western Films? - (reply: 2)
  153. Please help with odd bands and smears on Western blot - (reply: 4)
  154. Western blot membrane imaging - (reply: 7)
  155. Detection of T7 RNA polymerase by WB - (reply: 6)
  156. Shift in the position of band onto the western blot - (reply: 3)
  157. confusing Western results - (reply: 5)
  158. aditional band on WB - (reply: 1)
  159. which ELISA-based or western blot-based apoptosis method to choose? - (reply: 4)
  160. Histone precipitation and western blotting - (reply: 6)
  161. How to prepare blood serum for western blot? - (reply: 3)
  162. TNF alpha and IL 1b Western Blot - (reply: 1)
  163. Large proteins in Western - (reply: 12)
  164. Detecting doublet bands in Western for eGFP control - (reply: 1)
  165. Phospho-protein vs total protein Western blot - (reply: 5)
  166. Western blot trouble shooting - (reply: 3)
  167. Why do we use sponges in the Western Transfer? - (reply: 5)
  168. No WB bands for H2AX at all...! - (reply: 10)
  169. any tips for doing Fluorescence Western Blot? - (reply: 2)
  170. Store western samples in NP-40 lysis buffer? - (reply: 1)
  171. Need help for prepping western samples with NP-40 - (reply: 7)
  172. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  173. Non-Reduced Western Blot - (reply: 5)
  174. can coommassie stained gel be western blot - (reply: 5)
  175. Wet transfer: western blot - (reply: 3)
  176. Help for western blot wet transfer blurry bands - (reply: 2)
  177. Uneven lane widths during western blots - (reply: 10)
  178. Using human serum as first antibody in Western/ELISA - (reply: 3)
  179. Strip Western Blotting Troubleshooting - (reply: 2)
  180. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
  181. A possible protocol for western blotting troubleshooting? - (reply: 1)
  182. Freezing cell lysates for westerns - (reply: 2)
  183. Western blot narrow bands - (reply: 1)
  184. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
  185. Western Blot Antibody Epitope - (reply: 1)
  186. Western blot band anaylisis - (reply: 3)
  187. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
  188. Problems with western blot detection - (reply: 1)
  189. Using serum as the sample in Western blot - (reply: 3)
  190. Puzzeling western blot results, missing band - (reply: 3)
  191. western blood immunodetection Ab - (reply: 1)
  192. stripping buffer for western blot - (reply: 3)
  193. Rapid western blot buffer ?? - (reply: 2)
  194. Western Blot problems-vertical streaking - (reply: 3)
  195. Problems with high molecular weight protein transfer in western blot - (reply: 1)
  196. Trouble Western Blotting a 360kDa Protein - (reply: 5)
  197. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
  198. many bands Western Blot - (reply: 2)
  199. Western blot does not match IF - (reply: 2)
  200. Far western blotting - (reply: 5)
  201. Western blot mystery - possibly a sample preparation problem? - (reply: 11)
  202. Western blot sample loading question - (reply: 1)
  203. sample precipitation problem in western blotting - (reply: 2)
  204. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
  205. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  206. Getting too Many Bands on my Western Blot - (reply: 9)
  207. Western transfer tank heating up - (reply: 2)
  208. Simple Western = a western blot revolution? - (reply: 2)
  209. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  210. Western Blotting - (reply: 10)
  211. brain and colon tissue homogenate for WB - (reply: 3)
  212. Detection of glycosylations on Western blotted proteins - (reply: 2)
  213. Problems with protein estimation and western blots - (reply: 1)
  214. western blot loading control - (reply: 2)
  215. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  216. Analyzing Western Bands by Densiometry - (reply: 2)
  217. Can FLAG IP, but cannot detect with Western - (reply: 4)
  218. Cloned sequence is not predicted size by western blot - (reply: 1)
  219. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  220. Western blot and isoelectric point - (reply: 1)
  221. Western Blot Developing Cassette - (reply: 1)
  222. Western Blotting Resolving Problem - (reply: 3)
  223. Western gels running super slow! - (reply: 3)
  224. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  225. WB parametric or non-parametric - (reply: 6)
  226. Tie-2 westerns high background black lanes - (reply: 13)
  227. Identically loaded western bands loose intensity from left to right - (reply: 3)
  228. eIF4G western blot - (reply: 3)
  229. smear bands in western blot - (reply: 12)
  230. Western blots are 'semi' quantitative? - (reply: 12)
  231. weird query for Western Blotting - (reply: 2)
  232. Quick/One-step/automated Western Blot - (reply: 8)
  233. Amount of antibody used in Western Blot - (reply: 1)
  234. WB Transfer: Voltage vs. Current - (reply: 7)
  235. Telomerase Western Blotting - (reply: 5)
  236. Western Blotting of Proteins less than 10 kDa - (reply: 1)
  237. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  238. Internal Standard for western blot - (reply: 1)
  239. Sox-2 antibody detection using Western - (reply: 10)
  240. western blotting - (reply: 1)
  241. Western blot not showing protein - (reply: 2)
  242. Western Blot not showing protein - (reply: 4)
  243. normalisation of western blot from kodak film - (reply: 1)
  244. Separating close bands in Western Blot - (reply: 3)
  245. Western blot on intestine samples - (reply: 2)
  246. Western Blot for 320KD Protein - (reply: 4)
  247. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
  248. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  249. Cell Lysis and Western Blotting - (reply: 1)
  250. Western Blot Two Proteins - (reply: 1)
  251. Western Blot Protein Length - (reply: 4)
  252. Western Blot no target bands, show Non-specific bands - (reply: 5)
  253. Western Blot no signal - (reply: 6)
  254. smearing in western blotting - (reply: 4)
  255. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
  256. Comparison between Western blot, Immunofluorescent, Immunohistochemistry and ELI - Protein detection/separation methods (reply: 1)
  257. Protein agregation in WB samples - (reply: 3)
  258. what caused bubble band of my WB? - (reply: 4)
  259. asking suggestions for western blot - (reply: 3)
  260. Strange bands and large smear in western blot - (reply: 4)
  261. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  262. Dark western blot film - (reply: 12)
  263. Bradford Vs beta-actin for WB protein quantification - (reply: 3)
  264. Fuzzy bands in western blot - (reply: 2)
  265. SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
  266. I am desperated with my WB!! - (reply: 2)
  267. western blot of insulin receptor - (reply: 1)
  268. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
  269. WB - Transferring REALLY big proteins - (reply: 2)
  270. Western Blot Statistics - (reply: 2)
  271. Oscillation on my Western Blot results - (reply: 3)
  272. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
  273. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  274. western blot bands wider at the middle than the top - (reply: 2)
  275. Concentrated conditioned medium for western blot - (reply: 1)
  276. can't get bands in Western Blot - (reply: 5)
  277. Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
  278. Converging lanes in Western - (reply: 8)
  279. Huge background in Western blot - Studying the histone modification (reply: 1)
  280. Actin Bands smiling on western blot - (reply: 3)
  281. Boiling, protein aggregation......reversal?? (western) - (reply: 3)
  282. CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
  283. stripping of Western blot? - how does it work (reply: 1)
  284. Imaging western blot membrane - (reply: 2)
  285. a high background of western - a high background of western (reply: 2)
  286. western blotting at high current - (reply: 1)
  287. Western Blot of Large Protein Help - (reply: 1)
  288. Fluorescent Western Blotting Help - (reply: 8)
  289. Fusion tagged protein and Western Blot problem - (reply: 3)
  290. Western blot of apoptotic cells - (reply: 2)
  291. Quantification of proteins in Western Bands - (reply: 2)
  292. Western didnot work should I strip blot if i want to detect same protein-Notch I - (reply: 1)
  293. troublshooting WB - (reply: 1)
  294. Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
  295. western blot - substarate buffer recepie (reply: 2)
  296. Cell Lysates from a Western Blog - (reply: 2)
  297. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
  298. The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 3)
  299. how to remove aspecifics on western blot filter - (reply: 2)
  300. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
  301. western without tween - (reply: 3)
  302. PI needs something ASAP! - densitometry on westerns (reply: 1)
  303. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
  304. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  305. Western for very small protein - (reply: 3)
  306. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  307. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
  308. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
  309. Running a western with cell media in gels? - (reply: 11)
  310. LC3 antibody advice - LC3 western blot (reply: 4)
  311. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
  312. Western unresolved lanes - (reply: 2)
  313. Doing WB from scratch for the first time. Need some info. - (reply: 2)
  314. Western blot bovine collagen typ I problem - (reply: 3)
  315. ECL visualization method in WB - (reply: 1)
  316. High background using 5% BSA for blocking Western Blot - (reply: 3)
  317. Western difficulty in primary neuron cells. - (reply: 1)
  318. Filming membrane (Western Blot & ECL) - (reply: 1)
  319. low signal in WB - (reply: 3)
  320. western blot is not consistent with immunofluorescence - (reply: 2)
  321. Western Blot - Input vs IP - (reply: 1)
  322. I see bands with western blot only the third day! - (reply: 2)
  323. Western Blots on Membrane Proteins - (reply: 9)
  324. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
  325. Nucleic Acid interference in Western Blot / Optimal Nuclear Extract Protocol - Nuclear Extract Optimization (reply: 4)
  326. western blot - (reply: 4)
  327. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
  328. two bands detected in western - (reply: 1)
  329. Strange Western blot background - (reply: 2)
  330. loading buffer and western blot help! - (reply: 5)
  331. "Sandy WB" - (reply: 9)
  332. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  333. Strange SDS gel after Western Blotting - (reply: 1)
  334. Western blot data analysis - (reply: 1)
  335. Western blot fail, time and time again - (reply: 21)
  336. Western blot of urine samples - Problems verifying loading (reply: 1)
  337. IL-6 antibody - Which IL-6 antibody to use in Wb (reply: 2)
  338. Western blot transfer weak - (reply: 1)
  339. Quantitative Western Imaging - (reply: 3)
  340. dot blot works, western doesn't - HELP! - (reply: 5)
  341. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  342. too much bands in western - (reply: 2)
  343. western blot to a metal mesh - (reply: 3)
  344. PVDF membrane dried after transfer (Western) - (reply: 6)
  345. Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
  346. No band after WB revelation - (reply: 2)
  347. how to check histone in western blot - (reply: 1)
  348. western blot woes - (reply: 2)
  349. Weak western signal for help - (reply: 13)
  350. Western not working anymore - (reply: 15)
  351. Western few questions - (reply: 1)
  352. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
  353. Antibody works in immunofluorescence, but not in WB - (reply: 4)
  354. B-actin as western blot -control - (reply: 4)
  355. horrific western blots , please help - (reply: 3)
  356. Collagen II western blotting - (reply: 1)
  357. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
  358. Western Blotting - Strange results (reply: 3)
  359. Does the tagged-protein always appear at the predicted size? - on western blot (reply: 1)
  360. western bands look funny - (reply: 5)
  361. HELP! WESTERN BLOTS SUDDENLY STOPPED WORKING! - (reply: 3)
  362. Western blot running and loading samples - (reply: 6)
  363. problems with His tagged protein western blots - (reply: 3)
  364. HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
  365. Different TBST pH's for WB incubation, why? - (reply: 1)
  366. Western Blot for low expressed protein - (reply: 4)
  367. Denaturing Reagent for Western Blot - (reply: 16)
  368. western exposure one part of band darker - (reply: 6)
  369. High protease activity in protein extraction - A problem in western blot (reply: 2)
  370. Film for Western Blots - (reply: 2)
  371. Western Troubleshooting Help, please! - (reply: 3)
  372. Re-use membrane in Western Blot - (reply: 3)
  373. Dot blot- great, western blot - no signal - (reply: 3)
  374. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  375. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  376. basic question in SDS and western blot - (reply: 1)
  377. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  378. Western blot materials - sealed plastic (reply: 2)
  379. anti-mouse CD11b in WB - Has anybody used an Ab aganist CD11b that works in WB? (reply: 2)
  380. Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
  381. ugly merged bands in western blot - (reply: 3)
  382. bad western blot! what is the cause? - (reply: 3)
  383. Western Blot Data Analysis - (reply: 2)
  384. Western blot for muscle - (reply: 2)
  385. Identify the band in western blot - (reply: 5)
  386. Need help with Caspase WB - (reply: 2)
  387. Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
  388. Detecting BDNF with WB... - troubleshooting... (reply: 2)
  389. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
  390. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
  391. western blot using culture media - (reply: 4)
  392. Re-inserting marker into scanned WB - (reply: 1)
  393. Functions of BSA and Tween 20 in western blotting - (reply: 2)
  394. develop western by ECL plus - (reply: 1)
  395. Western with conjugated antibody? - (reply: 2)
  396. primary antibody for western - (reply: 6)
  397. my western blots of membrane protein do not work - (reply: 9)
  398. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  399. About GFP- tagged proteins in western blot - (reply: 1)
  400. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
  401. western blot sample in high salt concentration - (reply: 6)
  402. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  403. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  404. how to get rabbit antibody for Western blotting - (reply: 2)
  405. western blot - inconsistent western blot (reply: 6)
  406. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
  407. Western blotting - (reply: 4)
  408. Use of cold milk??? - Western blot (reply: 2)
  409. Western blotting for proteins in vesicles? - (reply: 2)
  410. Help with Western Blotting of Keratin Proteins - (reply: 2)
  411. Western bands fading out, pleeeese help - (reply: 1)
  412. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  413. Protein won't transfer for Western Blot - (reply: 5)
  414. cytochrome C Western Blot - (reply: 7)
  415. Looking for Abtibody Suppliers/Manufacturers - Hycridoma, monoclonal, manufacture, Antibody, mAb, ELISA, Western Blot (reply: 2)
  416. IFS is positive and WB negative - (reply: 1)
  417. immunofluorescence and western blotting - (reply: 3)
  418. Immunoblotting - (reply: 7)
  419. storing WB blots in PBS-tween - (reply: 7)
  420. Fluorescent visualization of Western blot - (reply: 2)
  421. Protein present in Western, not ELISA - (reply: 10)
  422. PDI western blot - can't reduce the disulfide bonds (reply: 5)
  423. the sensitive amount of protein for western blotting - (reply: 3)
  424. How to determine final amount of 20 ng protein per WB well - (reply: 8)
  425. Western blotting and immunofluorescence results don't correspond to each oth - (reply: 2)
  426. protein size change after western blot - (reply: 1)
  427. Western blot protocol - (reply: 2)
  428. Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
  429. Native PAGE w. Western protein marker - (reply: 4)
  430. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  431. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  432. western blotting problem with detection - signal fades away quickly (reply: 2)
  433. Two bands for p53 on Western Blot - (reply: 7)
  434. Overblocking Western? - (reply: 5)
  435. Preparation of tree tissue for western blot - (reply: 1)
  436. NFkB western blotting - (reply: 3)
  437. DyLight vs. IRDye Western Blot Li-Cor Odyssey - Flourescent labled antibody DyLight vs IRDye (reply: 1)
  438. Western Blot for Bacteria - Loading Control (reply: 1)
  439. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  440. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  441. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
  442. IP and western blot - (reply: 5)
  443. western blot - problem regrding western blotting (reply: 1)
  444. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
  445. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
  446. WB reference - Good reference for WB (reply: 6)
  447. western blot problem: cell culture VS mouse tissue - (reply: 4)
  448. Equal loading for Supernatant western blots??? - (reply: 1)
  449. western blotting loading control - (reply: 5)
  450. Using Photoshop etc on Western bot images - (reply: 8)
  451. Ghost bands in western blot - Western blotting problems (reply: 9)
  452. Why immunoblotting of actin coming at higher Molec. Weight - (reply: 1)
  453. x-ray films vs direct imaging - Preferred method for western development (reply: 17)
  454. beginner's Questions on Western Blotting - (reply: 4)
  455. White speckles on western blots - Why?? :( (reply: 3)
  456. Weak signal in WESTERN BLOT - Western Blot problem (reply: 1)
  457. SDF-1(CXCL12) western blot - (reply: 13)
  458. western blot - western blot (reply: 17)
  459. handling retinal tissue for western blot analysis - (reply: 4)
  460. Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
  461. actin MW problem in western blot - (reply: 4)
  462. A single band on WB smaller than expected - (reply: 3)
  463. WB does not correspond to protein array - (reply: 1)
  464. Quantifying Western blots - (reply: 1)
  465. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
  466. Strange bands in western of RPE-1 cells - (reply: 1)
  467. Looking for new endogenous control for WB - (reply: 3)
  468. western blot problems - western blot troubleshooting (reply: 3)
  469. Co-IP and Western blot with snap-frozen brain tissues - (reply: 2)
  470. Western Blotting - (reply: 1)
  471. Same Non Specific Banding on Western using HA, FLAG and GFP detection - (reply: 7)
  472. Immunofluorescence and Western Blot don't match - (reply: 5)
  473. Western Blot and Odyssey Image Software - (reply: 1)
  474. western blot gamma interferon - weak signal & denaturation (reply: 4)
  475. problem with Western blot loadig control - (reply: 2)
  476. western blotting at 37oC? - is it possible? (reply: 1)
  477. VEGF for western blot - (reply: 2)
  478. starry sky western blot... lots of spots. - (reply: 5)
  479. Western blot has alot of spots - (reply: 5)
  480. Tips on re-use of primary antibody in Western blot - (reply: 14)
  481. Protein much larger on Western blot than predicted - (reply: 12)
  482. how to deal with PVDF filter after WB - (reply: 5)
  483. western for methylated histone - (reply: 7)
  484. gel shift in western blot - (reply: 3)
  485. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
  486. Multiple antibody blotting - western blot - (reply: 16)
  487. Is immunoblotting possible after the gel has been silver stained - (reply: 2)
  488. Could this liver homogenate be used for Western Blot? - (reply: 2)
  489. Western Blotting- A beginner Guide - Abcam (reply: 3)
  490. PVDF vs. Nitrocellulose for Western blot - (reply: 20)