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Western blotting Related Discussions
  1. Can anyone help me with Western blotting problem? - (reply: 5)
  2. western blotting troubleshooting - (reply: 3)
  3. Question on western blot picture - (reply: 2)
  4. how to interpret this WB result - (reply: 7)
  5. SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample - (reply: 1)
  6. coIP followed by a Western Blot - (reply: 4)
  7. WB imaging on Vilber-Lourmat Fusion - (reply: 1)
  8. Low weight protein degradation during western blot - (reply: 1)
  9. Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
  10. phospho-creb western blotting - (reply: 3)
  11. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
  12. Non-specific binding of protein in western blot - (reply: 3)
  13. 2D PAGE and Western blotting - (reply: 1)
  14. Lane spillover (?) on Western Blot - (reply: 3)
  15. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
  16. No band detected in WB - (reply: 4)
  17. Western Blot diffuse bands - (reply: 3)
  18. Human cell membrane protein Western blot! - (reply: 2)
  19. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
  20. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
  21. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
  22. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
  23. what should be used as controls during western blotting? - (reply: 4)
  24. Dumbbell-shaped bands on the WB - (reply: 3)
  25. Help in western blotting GAPDH bands are not even - (reply: 1)
  26. 37kb and 7kb protein in western blot - (reply: 24)
  27. Dot blot/Western background signal problem - (reply: 1)
  28. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
  29. What is best to measure protein expression: western blot or ELISA? - (reply: 4)
  30. Two bands in WB but one band in IP - (reply: 1)
  31. Two bands in WB but one band in IP - (reply: 2)
  32. Western blot detects exogenous protein but not endogenous - (reply: 2)
  33. White bands in western blotting - (reply: 4)
  34. How to obtain a control positive for my WB - (reply: 3)
  35. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  36. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  37. Question on loss of signal between samples in western blot - (reply: 4)
  38. Sample prep of cells for FACS analysis and running WB - (reply: 4)
  39. PCR & Western Blot sample preperation - (reply: 2)
  40. PCR & Western Blot sample preperation - (reply: 5)
  41. How to optimise my western semi-dry transfer? - (reply: 3)
  42. Help on native PAGE gel and its western blot result - (reply: 5)
  43. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  44. No protein on western blot but standard transferred - (reply: 2)
  45. Can't detect GAPDH (western) with Alexa488 secondary antibody - (reply: 6)
  46. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
  47. how to choose a proper tag for western blotting - (reply: 6)
  48. Strange band pattern in Western Blot - (reply: 1)
  49. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  50. RIPA buffer for IP and western - (reply: 1)
  51. Problems of western signal - (reply: 5)
  52. Western Blot Help - (reply: 1)
  53. Western Blot: Unequal Sample Migration - (reply: 4)
  54. Western blot with smeared bands - (reply: 3)
  55. Blocking agent in western blotting of histone proteins - (reply: 1)
  56. Best western loading control for comparing different cell lines - (reply: 2)
  57. western blotting running problem? - (reply: 2)
  58. Keeping apoptotic/detached cells for WB? - (reply: 3)
  59. Advice with WB please - (reply: 3)
  60. Western Blot Wet Transfer Conditions - (reply: 10)
  61. Western Blot and Inmunofluorescence contradictions - (reply: 12)
  62. Nanog double band in WB - (reply: 1)
  63. Help with western blot!! - (reply: 1)
  64. Band on Western Blot are smeared - (reply: 1)
  65. Can Anti-IgG Horseradish peroxidase be used in a whole blood western blot assay? - (reply: 1)
  66. advice in western blotting - (reply: 1)
  67. can antibodies recommended for western blot be used for immunopreipitation - (reply: 2)
  68. Please help uneven bands in western blot - (reply: 9)
  69. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
  70. Cell Lysate Western Blot Little or No Signal - (reply: 7)
  71. Need Help with Western blot - (reply: 1)
  72. Western Blot Problems...too many unspecific bands - (reply: 4)
  73. IL 8 - western blotting - (reply: 1)
  74. how to make fast blocking buffer in western blot? - (reply: 1)
  75. Wester blot analysis - (reply: 5)
  76. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  77. timecourse experiment for Western and qPCR - (reply: 2)
  78. Western blotting - (reply: 4)
  79. Fixing concentration for western blot from MTT - (reply: 1)
  80. Western Blotting of Amyloid Beta - (reply: 1)
  81. Need help translating my western blot protocol into every language - (reply: 3)
  82. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
  83. Western blot to CD44? - (reply: 3)
  84. Suggestion for anti CD44 antibody for WB and IP? - (reply: 1)
  85. Problems with western membrane stripping - (reply: 2)
  86. western blot of cardiomyocytes - (reply: 2)
  87. Western blot using blood plasma - (reply: 5)
  88. Smear formed in western blots while using tubulin as control - (reply: 4)
  89. How to confirm the band of interest in Westerns blot - (reply: 6)
  90. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  91. Western after ChIP - how to do that? - (reply: 2)
  92. Western not working - (reply: 4)
  93. fused bands of beta-actin in western blot - (reply: 2)
  94. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
  95. Problem with GFP detection in western - (reply: 6)
  96. Wrong protein size on western blot - (reply: 10)
  97. How to know if the signal of western blot is saturated ? - (reply: 4)
  98. Uneven banding - Western blot troubleshooting - (reply: 4)
  99. Western Blot with fixed cells - (reply: 5)
  100. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
  101. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
  102. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
  103. 3 extra bands show up on my western after an IP - (reply: 2)
  104. about western blot analysis - (reply: 1)
  105. Western Blotting loading control for large proteins - (reply: 2)
  106. Western Blotting of Amyloid Beta - (reply: 3)
  107. Western Blotting of LRRK2 - (reply: 1)
  108. Protein of 460 kD on western blot - (reply: 3)
  109. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
  110. NCOR1 western - (reply: 8)
  111. Discrepancy between WB and Flow cytometry result - (reply: 5)
  112. Western blotting transfer - (reply: 2)
  113. Western Quantification (No BCA???) - (reply: 2)
  114. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  115. Proper western blotting - (reply: 6)
  116. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
  117. No bands/weird bands on western blot - (reply: 3)
  118. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
  119. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
  120. please help with my western blots! - (reply: 3)
  121. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  122. Western blot- bands weak - (reply: 11)
  123. Preparing supernatant from cell culture for western blot - (reply: 4)
  124. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
  125. Curved bands in Western Blot - (reply: 2)
  126. Western blot and immunofluorescence staining - (reply: 9)
  127. Western blot and Triton X-100 in blocking buffer - (reply: 1)
  128. Dot blot translation to WB - (reply: 2)
  129. western blotting troubleshooting - (reply: 2)
  130. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  131. EPIGENETICS : Help required ... Histone Western Blot - (reply: 1)
  132. Far western blot: probe concentration - (reply: 1)
  133. Western blot question- High % Gel/Low MW Protein - (reply: 7)
  134. western blot explenation - (reply: 8)
  135. WB of cellular proteins in human serum - (reply: 2)
  136. Western blotting - (reply: 9)
  137. Phosphotyrosine western blotting, HELP! - (reply: 1)
  138. General chat about tween-20 as a WB block and wash - (reply: 1)
  139. western blot troubleshooting - (reply: 5)
  140. Membrane Protein SDS PAGE and WB - (reply: 1)
  141. western blot and actin issue - (reply: 2)
  142. Separating proteing with difference of 5Kd on western blot - (reply: 4)
  143. Immunoprecipitated protein visible on coomassie but not detected by Western - (reply: 4)
  144. Western Blot, Bax, Many bands - (reply: 3)
  145. Eliminating Protein/Protein Interactions for Western Blot - (reply: 2)
  146. Western Blot for Histone 3 K27me? - (reply: 1)
  147. Western Blot v ELISA for Different Samples? - (reply: 1)
  148. CCRK (CDK20) protein expression and western blot - (reply: 7)
  149. IGF2 antibody for immunohistochemistry/WB in mouse? - (reply: 2)
  150. SDS Page and WB: Wrong size bands - (reply: 2)
  151. Good Scanner for Western Films? - (reply: 2)
  152. Please help with odd bands and smears on Western blot - (reply: 4)
  153. Western blot membrane imaging - (reply: 7)
  154. Detection of T7 RNA polymerase by WB - (reply: 6)
  155. Shift in the position of band onto the western blot - (reply: 3)
  156. confusing Western results - (reply: 5)
  157. aditional band on WB - (reply: 1)
  158. which ELISA-based or western blot-based apoptosis method to choose? - (reply: 4)
  159. Histone precipitation and western blotting - (reply: 6)
  160. How to prepare blood serum for western blot? - (reply: 3)
  161. TNF alpha and IL 1b Western Blot - (reply: 1)
  162. Large proteins in Western - (reply: 12)
  163. Detecting doublet bands in Western for eGFP control - (reply: 1)
  164. Phospho-protein vs total protein Western blot - (reply: 5)
  165. Western blot trouble shooting - (reply: 3)
  166. Why do we use sponges in the Western Transfer? - (reply: 5)
  167. No WB bands for H2AX at all...! - (reply: 10)
  168. any tips for doing Fluorescence Western Blot? - (reply: 2)
  169. Store western samples in NP-40 lysis buffer? - (reply: 1)
  170. Need help for prepping western samples with NP-40 - (reply: 7)
  171. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  172. Non-Reduced Western Blot - (reply: 5)
  173. can coommassie stained gel be western blot - (reply: 5)
  174. Wet transfer: western blot - (reply: 3)
  175. Help for western blot wet transfer blurry bands - (reply: 2)
  176. Uneven lane widths during western blots - (reply: 10)
  177. Using human serum as first antibody in Western/ELISA - (reply: 3)
  178. Strip Western Blotting Troubleshooting - (reply: 2)
  179. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
  180. A possible protocol for western blotting troubleshooting? - (reply: 1)
  181. Freezing cell lysates for westerns - (reply: 2)
  182. Western blot narrow bands - (reply: 1)
  183. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
  184. Western Blot Antibody Epitope - (reply: 1)
  185. Western blot band anaylisis - (reply: 3)
  186. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
  187. Problems with western blot detection - (reply: 1)
  188. Using serum as the sample in Western blot - (reply: 3)
  189. Puzzeling western blot results, missing band - (reply: 3)
  190. western blood immunodetection Ab - (reply: 1)
  191. stripping buffer for western blot - (reply: 3)
  192. Rapid western blot buffer ?? - (reply: 2)
  193. Western Blot problems-vertical streaking - (reply: 3)
  194. Problems with high molecular weight protein transfer in western blot - (reply: 1)
  195. Trouble Western Blotting a 360kDa Protein - (reply: 5)
  196. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
  197. many bands Western Blot - (reply: 2)
  198. Western blot does not match IF - (reply: 2)
  199. Far western blotting - (reply: 5)
  200. Western blot mystery - possibly a sample preparation problem? - (reply: 11)
  201. Western blot sample loading question - (reply: 1)
  202. sample precipitation problem in western blotting - (reply: 2)
  203. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
  204. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  205. Getting too Many Bands on my Western Blot - (reply: 9)
  206. Western transfer tank heating up - (reply: 2)
  207. Simple Western = a western blot revolution? - (reply: 2)
  208. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  209. Western Blotting - (reply: 10)
  210. brain and colon tissue homogenate for WB - (reply: 3)
  211. Detection of glycosylations on Western blotted proteins - (reply: 2)
  212. Problems with protein estimation and western blots - (reply: 1)
  213. western blot loading control - (reply: 2)
  214. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  215. Analyzing Western Bands by Densiometry - (reply: 2)
  216. Can FLAG IP, but cannot detect with Western - (reply: 4)
  217. Cloned sequence is not predicted size by western blot - (reply: 1)
  218. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
  219. Western blot and isoelectric point - (reply: 1)
  220. Western Blot Developing Cassette - (reply: 1)
  221. Western Blotting Resolving Problem - (reply: 3)
  222. Western gels running super slow! - (reply: 3)
  223. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  224. WB parametric or non-parametric - (reply: 6)
  225. Tie-2 westerns high background black lanes - (reply: 13)
  226. Identically loaded western bands loose intensity from left to right - (reply: 3)
  227. eIF4G western blot - (reply: 3)
  228. smear bands in western blot - (reply: 12)
  229. Western blots are 'semi' quantitative? - (reply: 12)
  230. weird query for Western Blotting - (reply: 2)
  231. Quick/One-step/automated Western Blot - (reply: 8)
  232. Amount of antibody used in Western Blot - (reply: 1)
  233. WB Transfer: Voltage vs. Current - (reply: 7)
  234. Telomerase Western Blotting - (reply: 5)
  235. Western Blotting of Proteins less than 10 kDa - (reply: 1)
  236. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  237. Internal Standard for western blot - (reply: 1)
  238. Sox-2 antibody detection using Western - (reply: 10)
  239. western blotting - (reply: 1)
  240. Western blot not showing protein - (reply: 2)
  241. Western Blot not showing protein - (reply: 4)
  242. normalisation of western blot from kodak film - (reply: 1)
  243. Separating close bands in Western Blot - (reply: 3)
  244. Western blot on intestine samples - (reply: 2)
  245. Western Blot for 320KD Protein - (reply: 4)
  246. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
  247. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  248. Cell Lysis and Western Blotting - (reply: 1)
  249. Western Blot Two Proteins - (reply: 1)
  250. Western Blot Protein Length - (reply: 4)
  251. Western Blot no target bands, show Non-specific bands - (reply: 5)
  252. Western Blot no signal - (reply: 6)
  253. smearing in western blotting - (reply: 4)
  254. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
  255. Comparison between Western blot, Immunofluorescent, Immunohistochemistry and ELI - Protein detection/separation methods (reply: 1)
  256. Protein agregation in WB samples - (reply: 3)
  257. what caused bubble band of my WB? - (reply: 4)
  258. asking suggestions for western blot - (reply: 3)
  259. Strange bands and large smear in western blot - (reply: 4)
  260. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
  261. Dark western blot film - (reply: 12)
  262. Bradford Vs beta-actin for WB protein quantification - (reply: 3)
  263. Fuzzy bands in western blot - (reply: 2)
  264. SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
  265. I am desperated with my WB!! - (reply: 2)
  266. western blot of insulin receptor - (reply: 1)
  267. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
  268. WB - Transferring REALLY big proteins - (reply: 2)
  269. Western Blot Statistics - (reply: 2)
  270. Oscillation on my Western Blot results - (reply: 3)
  271. Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
  272. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  273. western blot bands wider at the middle than the top - (reply: 2)
  274. Concentrated conditioned medium for western blot - (reply: 1)
  275. can't get bands in Western Blot - (reply: 5)
  276. Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
  277. Converging lanes in Western - (reply: 8)
  278. Huge background in Western blot - Studying the histone modification (reply: 1)
  279. Actin Bands smiling on western blot - (reply: 3)
  280. Boiling, protein aggregation......reversal?? (western) - (reply: 3)
  281. CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
  282. stripping of Western blot? - how does it work (reply: 1)
  283. Imaging western blot membrane - (reply: 2)
  284. a high background of western - a high background of western (reply: 2)
  285. western blotting at high current - (reply: 1)
  286. Western Blot of Large Protein Help - (reply: 1)
  287. Fluorescent Western Blotting Help - (reply: 8)
  288. Fusion tagged protein and Western Blot problem - (reply: 3)
  289. Western blot of apoptotic cells - (reply: 2)
  290. Quantification of proteins in Western Bands - (reply: 2)
  291. Western didnot work should I strip blot if i want to detect same protein-Notch I - (reply: 1)
  292. troublshooting WB - (reply: 1)
  293. Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
  294. western blot - substarate buffer recepie (reply: 2)
  295. Cell Lysates from a Western Blog - (reply: 2)
  296. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
  297. The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 3)
  298. how to remove aspecifics on western blot filter - (reply: 2)
  299. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
  300. western without tween - (reply: 3)
  301. PI needs something ASAP! - densitometry on westerns (reply: 1)
  302. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
  303. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  304. Western for very small protein - (reply: 3)
  305. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  306. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
  307. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
  308. Running a western with cell media in gels? - (reply: 11)
  309. LC3 antibody advice - LC3 western blot (reply: 4)
  310. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
  311. Western unresolved lanes - (reply: 2)
  312. Doing WB from scratch for the first time. Need some info. - (reply: 2)
  313. Western blot bovine collagen typ I problem - (reply: 3)
  314. ECL visualization method in WB - (reply: 1)
  315. High background using 5% BSA for blocking Western Blot - (reply: 3)
  316. Western difficulty in primary neuron cells. - (reply: 1)
  317. Filming membrane (Western Blot & ECL) - (reply: 1)
  318. low signal in WB - (reply: 3)
  319. western blot is not consistent with immunofluorescence - (reply: 2)
  320. Western Blot - Input vs IP - (reply: 1)
  321. I see bands with western blot only the third day! - (reply: 2)
  322. Western Blots on Membrane Proteins - (reply: 9)
  323. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
  324. Nucleic Acid interference in Western Blot / Optimal Nuclear Extract Protocol - Nuclear Extract Optimization (reply: 4)
  325. western blot - (reply: 4)
  326. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
  327. two bands detected in western - (reply: 1)
  328. Strange Western blot background - (reply: 2)
  329. loading buffer and western blot help! - (reply: 5)
  330. "Sandy WB" - (reply: 9)
  331. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  332. Strange SDS gel after Western Blotting - (reply: 1)
  333. Western blot data analysis - (reply: 1)
  334. Western blot fail, time and time again - (reply: 21)
  335. Western blot of urine samples - Problems verifying loading (reply: 1)
  336. IL-6 antibody - Which IL-6 antibody to use in Wb (reply: 2)
  337. Western blot transfer weak - (reply: 1)
  338. Quantitative Western Imaging - (reply: 3)
  339. dot blot works, western doesn't - HELP! - (reply: 5)
  340. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  341. too much bands in western - (reply: 2)
  342. western blot to a metal mesh - (reply: 3)
  343. PVDF membrane dried after transfer (Western) - (reply: 6)
  344. Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
  345. No band after WB revelation - (reply: 2)
  346. how to check histone in western blot - (reply: 1)
  347. western blot woes - (reply: 2)
  348. Weak western signal for help - (reply: 13)
  349. Western not working anymore - (reply: 15)
  350. Western few questions - (reply: 1)
  351. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
  352. Antibody works in immunofluorescence, but not in WB - (reply: 4)
  353. B-actin as western blot -control - (reply: 4)
  354. horrific western blots , please help - (reply: 3)
  355. Collagen II western blotting - (reply: 1)
  356. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
  357. Western Blotting - Strange results (reply: 3)
  358. Does the tagged-protein always appear at the predicted size? - on western blot (reply: 1)
  359. western bands look funny - (reply: 5)
  360. HELP! WESTERN BLOTS SUDDENLY STOPPED WORKING! - (reply: 3)
  361. Western blot running and loading samples - (reply: 6)
  362. problems with His tagged protein western blots - (reply: 3)
  363. HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
  364. Different TBST pH's for WB incubation, why? - (reply: 1)
  365. Western Blot for low expressed protein - (reply: 4)
  366. Denaturing Reagent for Western Blot - (reply: 16)
  367. western exposure one part of band darker - (reply: 6)
  368. High protease activity in protein extraction - A problem in western blot (reply: 2)
  369. Film for Western Blots - (reply: 2)
  370. Western Troubleshooting Help, please! - (reply: 3)
  371. Re-use membrane in Western Blot - (reply: 3)
  372. Dot blot- great, western blot - no signal - (reply: 3)
  373. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  374. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  375. basic question in SDS and western blot - (reply: 1)
  376. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  377. Western blot materials - sealed plastic (reply: 2)
  378. anti-mouse CD11b in WB - Has anybody used an Ab aganist CD11b that works in WB? (reply: 2)
  379. Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
  380. ugly merged bands in western blot - (reply: 3)
  381. bad western blot! what is the cause? - (reply: 3)
  382. Western Blot Data Analysis - (reply: 2)
  383. Western blot for muscle - (reply: 2)
  384. Identify the band in western blot - (reply: 5)
  385. Need help with Caspase WB - (reply: 2)
  386. Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
  387. Detecting BDNF with WB... - troubleshooting... (reply: 2)
  388. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
  389. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
  390. western blot using culture media - (reply: 4)
  391. Re-inserting marker into scanned WB - (reply: 1)
  392. Functions of BSA and Tween 20 in western blotting - (reply: 2)
  393. develop western by ECL plus - (reply: 1)
  394. Western with conjugated antibody? - (reply: 2)
  395. primary antibody for western - (reply: 6)
  396. my western blots of membrane protein do not work - (reply: 9)
  397. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  398. About GFP- tagged proteins in western blot - (reply: 1)
  399. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
  400. western blot sample in high salt concentration - (reply: 6)
  401. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  402. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  403. how to get rabbit antibody for Western blotting - (reply: 2)
  404. western blot - inconsistent western blot (reply: 6)
  405. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
  406. Western blotting - (reply: 4)
  407. Use of cold milk??? - Western blot (reply: 2)
  408. Western blotting for proteins in vesicles? - (reply: 2)
  409. Help with Western Blotting of Keratin Proteins - (reply: 2)
  410. Western bands fading out, pleeeese help - (reply: 1)
  411. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  412. Protein won't transfer for Western Blot - (reply: 5)
  413. cytochrome C Western Blot - (reply: 7)
  414. Looking for Abtibody Suppliers/Manufacturers - Hycridoma, monoclonal, manufacture, Antibody, mAb, ELISA, Western Blot (reply: 2)
  415. IFS is positive and WB negative - (reply: 1)
  416. immunofluorescence and western blotting - (reply: 3)
  417. Immunoblotting - (reply: 7)
  418. storing WB blots in PBS-tween - (reply: 7)
  419. Fluorescent visualization of Western blot - (reply: 2)
  420. Protein present in Western, not ELISA - (reply: 10)
  421. PDI western blot - can't reduce the disulfide bonds (reply: 5)
  422. the sensitive amount of protein for western blotting - (reply: 3)
  423. How to determine final amount of 20 ng protein per WB well - (reply: 8)
  424. Western blotting and immunofluorescence results don't correspond to each oth - (reply: 2)
  425. protein size change after western blot - (reply: 1)
  426. Western blot protocol - (reply: 2)
  427. Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
  428. Native PAGE w. Western protein marker - (reply: 4)
  429. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  430. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  431. western blotting problem with detection - signal fades away quickly (reply: 2)
  432. Two bands for p53 on Western Blot - (reply: 7)
  433. Overblocking Western? - (reply: 5)
  434. Preparation of tree tissue for western blot - (reply: 1)
  435. NFkB western blotting - (reply: 3)
  436. DyLight vs. IRDye Western Blot Li-Cor Odyssey - Flourescent labled antibody DyLight vs IRDye (reply: 1)
  437. Western Blot for Bacteria - Loading Control (reply: 1)
  438. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  439. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  440. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
  441. IP and western blot - (reply: 5)
  442. western blot - problem regrding western blotting (reply: 1)
  443. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
  444. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
  445. WB reference - Good reference for WB (reply: 6)
  446. western blot problem: cell culture VS mouse tissue - (reply: 4)
  447. Equal loading for Supernatant western blots??? - (reply: 1)
  448. western blotting loading control - (reply: 5)
  449. Using Photoshop etc on Western bot images - (reply: 8)
  450. Ghost bands in western blot - Western blotting problems (reply: 9)
  451. Why immunoblotting of actin coming at higher Molec. Weight - (reply: 1)
  452. x-ray films vs direct imaging - Preferred method for western development (reply: 17)
  453. beginner's Questions on Western Blotting - (reply: 4)
  454. White speckles on western blots - Why?? :( (reply: 3)
  455. Weak signal in WESTERN BLOT - Western Blot problem (reply: 1)
  456. SDF-1(CXCL12) western blot - (reply: 13)
  457. western blot - western blot (reply: 17)
  458. handling retinal tissue for western blot analysis - (reply: 4)
  459. Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
  460. actin MW problem in western blot - (reply: 4)
  461. A single band on WB smaller than expected - (reply: 3)
  462. WB does not correspond to protein array - (reply: 1)
  463. Quantifying Western blots - (reply: 1)
  464. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
  465. Strange bands in western of RPE-1 cells - (reply: 1)
  466. Looking for new endogenous control for WB - (reply: 3)
  467. western blot problems - western blot troubleshooting (reply: 3)
  468. Co-IP and Western blot with snap-frozen brain tissues - (reply: 2)
  469. Western Blotting - (reply: 1)
  470. Same Non Specific Banding on Western using HA, FLAG and GFP detection - (reply: 7)
  471. Immunofluorescence and Western Blot don't match - (reply: 5)
  472. Western Blot and Odyssey Image Software - (reply: 1)
  473. western blot gamma interferon - weak signal & denaturation (reply: 4)
  474. problem with Western blot loadig control - (reply: 2)
  475. western blotting at 37oC? - is it possible? (reply: 1)
  476. VEGF for western blot - (reply: 2)
  477. starry sky western blot... lots of spots. - (reply: 5)
  478. Western blot has alot of spots - (reply: 5)
  479. Tips on re-use of primary antibody in Western blot - (reply: 14)
  480. Protein much larger on Western blot than predicted - (reply: 12)
  481. how to deal with PVDF filter after WB - (reply: 5)
  482. western for methylated histone - (reply: 7)
  483. gel shift in western blot - (reply: 3)
  484. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
  485. Multiple antibody blotting - western blot - (reply: 16)
  486. Is immunoblotting possible after the gel has been silver stained - (reply: 2)
  487. Could this liver homogenate be used for Western Blot? - (reply: 2)
  488. Western Blotting- A beginner Guide - Abcam (reply: 3)
  489. PVDF vs. Nitrocellulose for Western blot - (reply: 20)