Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Western Blotting- A beginner Guide - Abcam (Feb/01/2009 )

Western Blotting - A beginner Guide by Abcam

-Minnie Mouse-

I probed my protein (CD82 of 31Kda ) but got band at 60Kda.. that too a single band. what could be the reason for it please help......

-Nitin k.Wadhwa-

it could be a dimer or, more likely, sds-page gives a decent estimate of molecular size for most proteins but some do not.


may be non specific, jus to confirm ip down ur protein and run simultaneoulsy along with the total lysate and probe it...may be you will get a solution...

good luck,