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basic question in SDS and western blot - (Apr/09/2010 )

Dear all,

What is the minimum amount (ng)o f protein that can be detected by SDS and WB?


I would imagine as little as 1-5ng can be detected but this must be a function of your antibody too. Better monoclonal antibodies, especially for epitope tags like HA and FLAG detect much better than polyclonal protein specific antibodies. Hope this helps.