Chemiluminescence vs. Fluorescence visualisation of western blot - (Jan/26/2014 )
Has anyone used a fluorescently-tagged secondary antibody instead of chemiluminescent secondaries to visualise bands on a western blot membrane?
Would it be more sensitive and hence more accurate compared to the conventional method of visualising bands?
Yes, and Yes...
The usual fluorescent antibodies used are infra-red range antibodies, and are purported to have a greater sensitivity and a more linear response over a wider range of antibody binding (i.e. no chemi reaction to take into account).