problem with Western blot loadig control - (Mar/19/2009 )
I just wanna ask a very basic question about the choice of loading control.
I want to detect an enzyme protein (estimate MW is around 30-45 KD) in bacteria with western blot, but I don't know what loading control could be used since according to my knowledge, actin, tubulin and GADDH is specific for eukaryotes.
I'll be very apprecitive of any suggestion.
Thanks a lot!
I don't know what is normally used, but FYI, bacteria possess homologues for both tubulin and actin (FtsZ and mreB, respectively).
Prokaryotic origin of the actin cytoskeleton
FUSINITA VAN DEN ENT, LINDA A. AMOS & JAN LÖWE
Nature 413, 39-44 (6 September 2001)
Thanks a lot.
However, I guess it'll be better to use the loading control that was commonly used by others.
Has anyone ever used a loading control for the quantitative western blot detection of the bacteria protein?
I am waiting for any useful suggestions.