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Housekeeper for Plasma samples in Western Blotting - (Aug/03/2011 )

Hi Everyone

I'm currently trying to run a Western Blot on some Asthma and COPD serum samples. Since beta actin and GAPDH are secreted protiens and can't be used as the houskeeper, does anyone have any idea what could be used.

Any help would be greatly appreciated



how about the albumin?


blackstone on Thu Aug 4 06:56:19 2011 said:

how about the albumin?

Thanks - that was one I was considering


albumin concentration may not be consistent enough between samples to use for normalization