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any tips for doing Fluorescence Western Blot? - (Aug/12/2012 )

Hi guys, our lab decided to change our Western blot approach to fluorescence western blotting. Any tips for this newbie? Thanks

-rock&science-

Assuming that these are the far-red/infra-red based detection systems, there shouldn't be anything different to what you do normally, just follow the recommended antibody dilutions on the data sheets for testing them out.

-bob1-

thanks. I just feel nervous trying a new technique. figured I should ask here for tips.

-rock&science-