troublshooting WB - (Apr/03/2011 )
I run SDS page gels, using SDS is stacking and resolving gels.
When I run my samples, I used LDS (invitrogen)4X. Since I have some troubles of aspecifics on all the western blots I m doing! Im wondering if by using LDS instead of loading dye with SDS is the cause of these aspecifics.
Possibly, but it is unlikely. The non-specifics are more likely to be at the antibody/blocking/detection stages than during the running of the gels.