Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

How to obtain a control positive for my WB - (Apr/01/2015 )

I am detecting Influenza protein (NP) in a set of samples. I would like to have a control +ve for my WB. Now I thought to clone the NP gene in a plasmid that express it. But how to show the protein of the NP in WB ? 


Could you list some methods as to how to get a Ctr +ve for my WB ? 

-Mohamed 1984-

If you can express if off a plasmid and can get (or make) an antibody against the protein, that you can then show detects a band of the correct size and can be competed by peptide, then that is usually considered sufficient.


this is a little off topic:



-Mohamed 1984-