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How to obtain a control positive for my WB - (Apr/01/2015 )

I am detecting Influenza protein (NP) in a set of samples. I would like to have a control +ve for my WB. Now I thought to clone the NP gene in a plasmid that express it. But how to show the protein of the NP in WB ? 

 

Could you list some methods as to how to get a Ctr +ve for my WB ? 

-Mohamed 1984-

If you can express if off a plasmid and can get (or make) an antibody against the protein, that you can then show detects a band of the correct size and can be competed by peptide, then that is usually considered sufficient.

-bob1-

this is a little off topic:

 

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840294/

-BMF-

Thanks

-Mohamed 1984-