Words, Words, Words - it's only words (Jul/21/2009 )
package holiday
I suppose that makes it better, and think next week- Moanday is a holiday in Englandshire!
bus tour
did the westerns work?
I want holidays too.
magical
westerns kinda worked. didn't get the results i wanted tho. bit of misinformation on the building up, was looking for a protein in very low concentration that we probably wouldn't detect anyway but was the one generating a pattern in the previous run up. things not clear cut with proteins, sometimes they're up, sometimes they're down, apparently independent of the treatment applied.
when you taking holidays v?
magician
ok, this is probably a really stupid question... can you grow plant cells in a dish?
holidays in... december? maybe. nothing certain.
egypcian
as far as i'm aware, it is possible to grow calluses in vitro. we did that in a practical during my undergrad. no stupid question v
going back to oz?
walk like an egyptian
see, the only plant stuff i did at uni was cutting thin sections and staining them. i think i also may have grown an orchid in a glass tube.
any chance the weird western protein up down thing is due to the cell cycle?
yup, back to oz, and then maybe somewhere interesting.
99 luftballons
cell cycle might be involved, but that wouldn't be the main reason i would look at. how familiar are you with sumolation of proteins? are they really unstable?
Nena
SUMO is an awesome protein to work with (simply because ppt will always contain a sumo wrestler).
not an expert, but there are people in the office who work with it.
hypoxia causes sumolation of HIF1alpha, which allows it to bind to a ubiquitin ligase. then something else happens with another protein (magic), which leads to ubiquitination and then degradation of HIF1alpha. ta dah, almost everything i know about sumo.
I'm under the impression that sumo proteins are stable... i do know that sumo makes (some) other proteins more stable (HIF1alpha, and there's another one which is a bunch of numbers).
Nana
hey guys...a happy Moanday to all....hi V, Lost-san...hola tj....I think LQ is working on sumolation, tj....you can probably write him a pm and try to get him to come back here......
banana
i still have to read lots more about the paths of sumo. if i'm not mistaken, depending on the level of sumolation of the proteins they can become more stable or get degraded