Words, Words, Words - it's only words (Jul/21/2009 )
sky captain
back on sunday...
master and commander
i should probably be running another gel, but my optimism is low after last nights results...
the far side of the world
run the gel. have you got people to trouble shoot with?
encounters at the end of the world
i'll run the gels, just need to get about 1 l of methanol for downstream procedures because we ran out of it... i'll ask the pi of this lab for troubleshooting.
The Restaurant at the End of the Universe
Ok, why not start here... how was the protein made?
Do you have a positive control? or do you have access to a positive control?
mcdonald's
thanks v, I do have a recombinant protein that I've tested but it binds everywhere. Out was originally designed for arabidopsis and I checked the homology with rice and there is enough to make it work, I'm just trying over and over again, thing is I don't have loads of time before I have to back.
happy meal
ok... when it doesn't work, do you mean heaps of bands that make no sense, or no bands at all?
happy face
no bands at all after revealing on film. then i stain with coomasie blue and there are loads of bands... but probably not the one i'm interested in.
Unhappy Happiness
hi folks, TJ proteins suck so much more compared to dna, good luck...
smiley
hey guys....and weekend is almost here.......hola tj...ah...the western blues....
hey dr H...he'll get the hang of it...at least before his first grandson is born.......