Protocol Online logo
Top : Forum Archives: : Indexed by Topic
RNAi Related Discussions
  1. siRNA modification to make it more hydrophobic - (reply: 1)
  2. siRNA designation for different genes - (reply: 1)
  3. Double SiRNA trasfection - (reply: 1)
  4. siRNA into RAW264.7 cells? - any ideas (reply: 4)
  5. Sirna concentration - (reply: 1)
  6. suggested siRNA concentrations - (reply: 1)
  7. RNAi of non-coding RNAs....? - (reply: 6)
  8. Invitrogen's Negative control siRNA - (reply: 3)
  9. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
  10. Dissolve siRNA in non-RNase free buffer - (reply: 1)
  11. CD4 siRNA - (reply: 1)
  12. Optimization of siRNA experiments - (reply: 1)
  13. Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
  14. Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES? (reply: 3)
  15. siRNA toxicity in MCF7 cells - why are they sick????? (reply: 1)
  16. siRNA in organotypic cultures - siRNA-mediated gene silencing in vitro and in vivo (reply: 6)
  17. Possible transfect siRNA using Calcium Phosphete? - siRNA transfection (reply: 2)
  18. siRNA vs. miRNA - (reply: 5)
  19. HUVEC siRNA transfection help please - I need suggestions on what else to try! (reply: 3)
  20. test the knockdown effficiency with siRNA before transfection with lentivirus? - (reply: 1)
  21. siRNA transfection in MLE12 cells - (reply: 3)
  22. siRNA for retinal pigment epithelial cells - (reply: 1)
  23. RNAi effect-timing - (reply: 1)
  24. RNAi of two different genes - (reply: 4)
  25. Scaling up siRNA transfection - (reply: 1)
  26. Can RNAi be used for silencing of non-coding RNAs? - (reply: 5)
  27. Co-Transfection siRNA and targeting construct - (reply: 3)
  28. Combinatorial RNAi - double gene knockdown - (reply: 4)
  29. siRNA transfection: DNA conc optimization problem - (reply: 1)
  30. siRNA transfection in endothelial cells - (reply: 3)
  31. siRNA plus drugs - weird cell morphology (reply: 3)
  32. double siRNA knockdown? - procedure for simultaneous silencing (reply: 17)
  33. transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
  34. RNAi side effects - (reply: 6)
  35. interaction of viral protein with RNAi machinery - (reply: 1)
  36. RNAi vector construction - Has anybody done it? (reply: 2)
  37. Should siRNA sequence be 100% complementary? - (reply: 4)
  38. siRNA transfection - (reply: 4)
  39. easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
  40. RNAi rescue by ectopic expression - (reply: 2)
  41. siRNA transfection in primary hepatocytes - (reply: 1)
  42. validated siRNA not working plz help - (reply: 4)
  43. Transfection of siRNA in suspension cells - (reply: 6)
  44. gene duplication - affected by siRNA? (reply: 1)
  45. cholesterol conjugated siRNA - (reply: 2)
  46. RNAi - possibilities and applications - (reply: 3)
  47. Has anybody ever used Dharmacon ON-TARGETplus SMARTpool siRNA? - (reply: 6)
  48. siRNA in primary lymphocyte B - Which reagent do you recomend? (reply: 2)
  49. good reagents for transfection of siRNA in Caco-2 - (reply: 1)
  50. Control RNAi ! - (reply: 2)
  51. Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
  52. siRNA "knock-out"/transfection "knock-in" - (reply: 10)
  53. Inheritance of RNAi - (reply: 4)
  54. Good Review of RNAi - (reply: 2)
  55. (newbie) what makes siRNA and miRNA different - (reply: 2)
  56. siRNA knock down did not work - protein expression knock down by siRNA (reply: 4)
  57. General List of Off Target Effects of siRNA - (reply: 1)
  58. newbie questions on RNAi / microRNAs - (reply: 3)
  59. siRNA transfection of TWO targets simultaneously - Any experience/advice? (reply: 1)
  60. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  61. knocking down mature miRNA with siRNA - is it possible to knockdown miRNA with siRNA? (reply: 4)
  62. siRNA positive control - (reply: 3)
  63. ask for RNAi vetor - (reply: 2)
  64. detect siRNA derived from shRNA construct - (reply: 5)
  65. RNAi or Antisense oligos for inhibition in vitro and in vivo - (reply: 1)
  66. SiRNA in MIN6 cells, nucleofection - (reply: 1)
  67. how to detect double strand siRNA - (reply: 2)
  68. siRNA stable cell lines - (reply: 6)
  69. SiRNA Transfection in 60mm plates - (reply: 2)
  70. siRNA transfection - (reply: 5)
  71. RNAi and interferon induction - (reply: 6)
  72. siRNA causing no protein knockdown - (reply: 1)
  73. Loss of RNAi after changing culture media? - (reply: 2)
  74. siRNA transfection - (reply: 3)
  75. validated siRNA doesn't work - (reply: 6)
  76. sirna half life - time of effect (reply: 2)
  77. siRNA cost - siRNA cost (reply: 4)
  78. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
  79. siRNA + DNA transfection - (reply: 3)
  80. mRNA expression low/no effect of the siRNA - (reply: 1)
  81. siRNA: in vivo delivery - (reply: 11)
  82. Cytoplasmic granulation after siRNA transfection - (reply: 3)
  83. how to evaluate the siRNA silence efficiency? - western or rtPCR (reply: 6)
  84. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
  85. shRNA versus siRNA - (reply: 6)
  86. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
  87. GFP siRNA transfection - readout - (reply: 5)
  88. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
  89. siRNA protocol in floating cells - (reply: 1)
  90. siRNA transfection problem - (reply: 4)
  91. U6, H1 siRNA - (reply: 1)
  92. siRNA transfection reagent - (reply: 9)
  93. siRNA and Hybond XL - any one use them? (reply: 1)
  94. beginners guide to stable RNAi transfection - (reply: 1)
  95. siRNA: how long will knock-down last - (reply: 6)
  96. miRNA targets in comparison to siRNA targets - (reply: 2)
  97. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
  98. Basic question about siRNA, shRNA..etc - What's the difference? (reply: 5)
  99. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
  100. Sources for vector-based siRNA against kinases? - (reply: 1)
  101. About the conc. use of the siRNA - (reply: 4)
  102. Is RNAi patented? - (reply: 4)
  103. is there any fast screening after siRNA knockdown of protein? - (reply: 3)
  104. siRNA and mices - What is the best way to in vivo siRNA transfer (reply: 6)
  105. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
  106. Time point for reduction of protein levels after RNAi - (reply: 1)
  107. RNAi doesn't work? - (reply: 5)
  108. Knocking down miRNA using siRNA - (reply: 5)
  109. Low transfection of siRNA into HaCaT cells. Please help - (reply: 1)
  110. shRNA/siRNA which loop sequence? - (reply: 2)
  111. shRNA/siRNA stem length 19, 20, or 21? - (reply: 3)
  112. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  113. siRNA in vivo use - (reply: 8)
  114. Off-target effects of RNAi - (reply: 4)
  115. How to increase RNAi efficiency by siRNA transfection? - (reply: 4)
  116. siRNA delivery in non-dividing cells - (reply: 8)
  117. RNA activation but not RNAi? - (reply: 8)
  118. How to trace siRNA in cells - (reply: 4)
  119. Time course for siRNA inhibition - When is the peak of inhibition after transfection ? (reply: 11)
  120. help with choosing model system for optimization of transfection of siRNA - and best deal for positive control? (reply: 5)
  121. RNAi of HepG2 cells - low transfection effeciency (reply: 1)
  122. shelf life of siRNA - (reply: 1)
  123. siRNA failure - Knock down failure with oligofectamine (reply: 5)
  124. Confirming SiRNA Knockdown - (reply: 6)
  125. siRNA duplex length, 27mer? - anyone tested that 27mer is better? (reply: 1)
  126. Increased transcripts after RNAi - (reply: 10)
  127. Difference between SiRNA and shRNA - (reply: 2)
  128. siRNA transfection of MIN6 cells - (reply: 2)
  129. siRNA extraction from plant material - (reply: 5)
  130. siRNA purification - (reply: 2)
  131. siRNA Cloning method - (reply: 2)
  132. Effect of scrambled siRNA on gene expression - (reply: 5)
  133. siRNA delivery into human primary macrophages - (reply: 3)
  134. Has anyone seen target mRNA increasing after RNAi? - (reply: 7)
  135. Tool for making scrambled control siRNA - (reply: 1)
  136. Position of RNAi target on mRNA - How important is the location (reply: 2)
  137. RNAi confusion - More expression! (reply: 7)
  138. RNAi in yeast? - (reply: 3)
  139. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
  140. siRNA in non-adherent cell lines? - Tips please (reply: 4)
  141. How to design siRNAs for RNAi - (reply: 2)
  142. How long DNA and siRNA stay in transfected cells - (reply: 2)
  143. A question on RNAi efficiency? - (reply: 5)
  144. how to start for effective RNAi screening--scale up question - (reply: 2)
  145. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
  146. How to know siRNA transfection efficiency? - (reply: 4)
  147. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
  148. about siRNA and shRNA - RNAi Vector (reply: 2)
  149. siRNA in vitro transcription-Ambion kit? - (reply: 3)
  150. siRNA to shRNA problem - (reply: 7)
  151. Question about doing RNAi in primary cells - (reply: 3)
  152. Knockdown protein expression using rnai - (reply: 4)
  153. How to extract siRNA from primordial leaf - (reply: 1)
  154. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
  155. what is the difference about siRAN, miRNA, shRNA and RNAi - (reply: 1)
  156. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  157. Basic questions about siRNA - (reply: 6)
  158. siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)
  159. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  160. RNAi in a sense/antisense transcript system - (reply: 3)
  161. siRNA transfection reagent for primary cultured cells - (reply: 2)
  162. Help me ! I want to do RNAi - (reply: 1)
  163. Control siRNA - (reply: 3)
  164. RNAi negative controls - (reply: 2)
  165. different siRNA for the same gene cause opposite effect - (reply: 6)
  166. nonspecific cellular responses - vector system vs synthetic siRNA - (reply: 1)
  167. siRNA Stable transfection in human fibroblast cells - (reply: 6)
  168. Quantitative gene knock down with siRNA - (reply: 1)
  169. Upcoming conferences? - RNAi conferences (reply: 3)
  170. Use of dual promoter for siRNA - (reply: 6)
  171. siRNA transfection in cardiomyocytes - siRNA transfection in neonatal cultured cardiomyocytes (reply: 1)
  172. How to choose the best optimum RNAi kit? - (reply: 1)
  173. siRNA kit - expensive (reply: 2)
  174. Designing RNAi construct for adult Drosophila - (reply: 1)
  175. siRNA databases and shRNA libraries - (reply: 8)
  176. siRNA /shRNA agains EGFP - (reply: 4)
  177. siRNA control by Western blot - how do I get equal loading concentration? (reply: 7)
  178. siRNA transfetion in MEL (murine erythroleukemia) cell - (reply: 3)
  179. RNAi using SIN-lentivectors BSL 2 plus? - (reply: 1)
  180. Is there article about lymphoma therapy with RNAi? - (reply: 1)
  181. negative control siRNA - (reply: 13)
  182. how to convert RNA-based siRNA to DNA-based siRNA? - (reply: 1)
  183. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
  184. Designing siRNA expression vectors - (reply: 2)
  185. About RNAi based on sh-RNA plasmid - (reply: 2)
  186. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
  187. siRNA overhangs - do they matter? (reply: 1)
  188. RNAi knockdown versus DNA knock-out - (reply: 2)
  189. qPCR and siRNA - (reply: 2)
  190. siRNA finder - (reply: 3)
  191. Transfection of keratinocytes with siRNA - (reply: 2)
  192. siRNA in Drosophila S2 cells? - (reply: 4)
  193. siRNA transfection - (reply: 1)
  194. methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
  195. RNAi in CHO cells - (reply: 5)
  196. siRNA northern - (reply: 1)
  197. No change in mRNA or protein levels after RNAi in Trypanosoma brucei - (reply: 4)
  198. siRNA versus shRNA - (reply: 4)
  199. Scrambled siRNA anyone? - (reply: 2)
  200. siRNA transfection of primary lymphocytes - (reply: 1)
  201. siRNA purity - (reply: 4)
  202. siRNA to shRNA - (reply: 7)
  203. Morphologic changes of HeLa cell after RNAi - (reply: 7)
  204. extraction of endogeneous mirna and sirna - (reply: 6)
  205. siRNA detection - Protocol for siRNA detection (reply: 3)
  206. Can I target non-coding sequence by siRNA - (reply: 6)
  207. SiRNA transfection in HEK293T - (reply: 1)
  208. Does siRNA transfection dependents on cell type - (reply: 2)
  209. siRNA transfection into neurons - (reply: 2)
  210. transfecting siRNA and a plasmid - (reply: 4)
  211. siRNA database - siRNA database (reply: 2)
  212. siRNA - augmentation instead of knockdown - siRNA - augmentation instead of knockdown (reply: 2)
  213. FITC-conjugated siRNA - (reply: 4)
  214. siRNA transfection into 3T3-L1 - (reply: 3)
  215. Genomic integration of siRNA expression cassette - Comparison of generated clones (reply: 3)
  216. siRNA for Akt1 - use of commercial siRNA to down-regulate Akt (reply: 4)
  217. siRNA design: where it should be targeted? - (reply: 5)
  218. How many is the limit? - What is the numerical limit to RNAi and others? (reply: 2)
  219. siRNA in embryo - (reply: 1)
  220. What is the different between microRNA and siRNA? - (reply: 4)
  221. How to transfect siRNA into the nucleus for transcriptional silencing? - Which transfection reagent? (reply: 9)
  222. How to transfect siRNA into suspension cell lines? - (reply: 3)
  223. siRNA detection - i'm totally lost (reply: 8)
  224. RNAi in Jurkat cells - (reply: 3)
  225. Antibiotics during siRNA transfection - (reply: 4)
  226. Different siRNA transfection agents - Have enyone tested them??? (reply: 44)
  227. RNAi with macrophage - (reply: 1)
  228. chemical synthesized siRNA and rapidly dividing cells - (reply: 2)
  229. about vectors and transfection of RNAi - (reply: 1)
  230. siRNA in vivo?? - (reply: 7)
  231. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
  232. several questions for siRNA target BLAST - (reply: 4)
  233. Is mRNA spliced with very small fragment treated with siRNA? - (reply: 3)
  234. RNAi vector for stable expression - (reply: 4)
  235. siRNA transfection - siRNA transfection (reply: 2)
  236. concentrate siRNA - (reply: 1)
  237. siRNA chemical synthesis - which company is reliable? (reply: 2)
  238. stable transfection with siRNA expression vector - (reply: 6)
  239. RNA Interference - how to produce siRNA (reply: 10)
  240. siRNA target selection problem - (reply: 11)