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RNAi Related Discussions
  1. siRNA designation for different genes - (reply: 1)
  2. siRNA into RAW264.7 cells? - any ideas (reply: 4)
  3. suggested siRNA concentrations - (reply: 1)
  4. Invitrogen's Negative control siRNA - (reply: 3)
  5. Dissolve siRNA in non-RNase free buffer - (reply: 1)
  6. Optimization of siRNA experiments - (reply: 1)
  7. Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES? (reply: 3)
  8. siRNA in organotypic cultures - siRNA-mediated gene silencing in vitro and in vivo (reply: 6)
  9. siRNA vs. miRNA - (reply: 5)
  10. test the knockdown effficiency with siRNA before transfection with lentivirus? - (reply: 1)
  11. siRNA for retinal pigment epithelial cells - (reply: 1)
  12. RNAi of two different genes - (reply: 4)
  13. Can RNAi be used for silencing of non-coding RNAs? - (reply: 5)
  14. Combinatorial RNAi - double gene knockdown - (reply: 4)
  15. siRNA transfection in endothelial cells - (reply: 3)
  16. double siRNA knockdown? - procedure for simultaneous silencing (reply: 17)
  17. RNAi side effects - (reply: 6)
  18. RNAi vector construction - Has anybody done it? (reply: 2)
  19. siRNA transfection - (reply: 4)
  20. RNAi rescue by ectopic expression - (reply: 2)
  21. validated siRNA not working plz help - (reply: 4)
  22. gene duplication - affected by siRNA? (reply: 1)
  23. RNAi - possibilities and applications - (reply: 3)
  24. siRNA in primary lymphocyte B - Which reagent do you recomend? (reply: 2)
  25. Control RNAi ! - (reply: 2)
  26. siRNA "knock-out"/transfection "knock-in" - (reply: 10)
  27. Good Review of RNAi - (reply: 2)
  28. siRNA knock down did not work - protein expression knock down by siRNA (reply: 4)
  29. newbie questions on RNAi / microRNAs - (reply: 3)
  30. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  31. siRNA positive control - (reply: 3)
  32. detect siRNA derived from shRNA construct - (reply: 5)
  33. SiRNA in MIN6 cells, nucleofection - (reply: 1)
  34. siRNA stable cell lines - (reply: 6)
  35. siRNA transfection - (reply: 5)
  36. siRNA causing no protein knockdown - (reply: 1)
  37. siRNA transfection - (reply: 3)
  38. sirna half life - time of effect (reply: 2)
  39. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
  40. mRNA expression low/no effect of the siRNA - (reply: 1)
  41. Cytoplasmic granulation after siRNA transfection - (reply: 3)
  42. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
  43. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
  44. siRNA transfection in suspended cell lines MV4-11, THP-1 - (reply: 1)
  45. siRNA transfection problem - (reply: 4)
  46. siRNA transfection reagent - (reply: 9)
  47. beginners guide to stable RNAi transfection - (reply: 1)
  48. miRNA targets in comparison to siRNA targets - (reply: 2)
  49. Basic question about siRNA, shRNA..etc - What's the difference? (reply: 5)
  50. Sources for vector-based siRNA against kinases? - (reply: 1)
  51. Is RNAi patented? - (reply: 4)
  52. siRNA and mices - What is the best way to in vivo siRNA transfer (reply: 6)
  53. Time point for reduction of protein levels after RNAi - (reply: 1)
  54. Knocking down miRNA using siRNA - (reply: 5)
  55. shRNA/siRNA which loop sequence? - (reply: 2)
  56. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  57. Off-target effects of RNAi - (reply: 4)
  58. siRNA delivery in non-dividing cells - (reply: 8)
  59. How to trace siRNA in cells - (reply: 4)
  60. help with choosing model system for optimization of transfection of siRNA - and best deal for positive control? (reply: 5)
  61. shelf life of siRNA - (reply: 1)
  62. Confirming SiRNA Knockdown - (reply: 6)
  63. Increased transcripts after RNAi - (reply: 10)
  64. siRNA transfection of MIN6 cells - (reply: 2)
  65. siRNA purification - (reply: 2)
  66. Effect of scrambled siRNA on gene expression - (reply: 5)
  67. Has anyone seen target mRNA increasing after RNAi? - (reply: 7)
  68. Position of RNAi target on mRNA - How important is the location (reply: 2)
  69. RNAi in yeast? - (reply: 3)
  70. siRNA in non-adherent cell lines? - Tips please (reply: 4)
  71. How long DNA and siRNA stay in transfected cells - (reply: 2)
  72. how to start for effective RNAi screening--scale up question - (reply: 2)
  73. How to know siRNA transfection efficiency? - (reply: 4)
  74. about siRNA and shRNA - RNAi Vector (reply: 2)
  75. siRNA to shRNA problem - (reply: 7)
  76. Knockdown protein expression using rnai - (reply: 4)
  77. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
  78. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  79. siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)
  80. RNAi in a sense/antisense transcript system - (reply: 3)
  81. Help me ! I want to do RNAi - (reply: 1)
  82. RNAi negative controls - (reply: 2)
  83. nonspecific cellular responses - vector system vs synthetic siRNA - (reply: 1)
  84. Quantitative gene knock down with siRNA - (reply: 1)
  85. Use of dual promoter for siRNA - (reply: 6)
  86. How to choose the best optimum RNAi kit? - (reply: 1)
  87. Designing RNAi construct for adult Drosophila - (reply: 1)
  88. siRNA /shRNA agains EGFP - (reply: 4)
  89. siRNA transfetion in MEL (murine erythroleukemia) cell - (reply: 3)
  90. Is there article about lymphoma therapy with RNAi? - (reply: 1)
  91. how to convert RNA-based siRNA to DNA-based siRNA? - (reply: 1)
  92. Designing siRNA expression vectors - (reply: 2)
  93. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
  94. RNAi knockdown versus DNA knock-out - (reply: 2)
  95. siRNA finder - (reply: 3)
  96. siRNA in Drosophila S2 cells? - (reply: 4)
  97. methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
  98. siRNA northern - (reply: 1)
  99. siRNA versus shRNA - (reply: 4)
  100. siRNA transfection of primary lymphocytes - (reply: 1)
  101. siRNA to shRNA - (reply: 7)
  102. extraction of endogeneous mirna and sirna - (reply: 6)
  103. Can I target non-coding sequence by siRNA - (reply: 6)
  104. Does siRNA transfection dependents on cell type - (reply: 2)
  105. transfecting siRNA and a plasmid - (reply: 4)
  106. siRNA - augmentation instead of knockdown - siRNA - augmentation instead of knockdown (reply: 2)
  107. siRNA transfection into 3T3-L1 - (reply: 3)
  108. siRNA for Akt1 - use of commercial siRNA to down-regulate Akt (reply: 4)
  109. How many is the limit? - What is the numerical limit to RNAi and others? (reply: 2)
  110. What is the different between microRNA and siRNA? - (reply: 4)
  111. How to transfect siRNA into suspension cell lines? - (reply: 3)
  112. RNAi in Jurkat cells - (reply: 3)
  113. Different siRNA transfection agents - Have enyone tested them??? (reply: 44)
  114. chemical synthesized siRNA and rapidly dividing cells - (reply: 2)
  115. siRNA in vivo?? - (reply: 7)
  116. several questions for siRNA target BLAST - (reply: 4)
  117. RNAi vector for stable expression - (reply: 4)
  118. concentrate siRNA - (reply: 1)
  119. stable transfection with siRNA expression vector - (reply: 6)
  120. siRNA target selection problem - (reply: 11)