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RNAi Related Discussions
siRNA designation for different genes -
(reply: 1)
siRNA into RAW264.7 cells? - any ideas
(reply: 4)
suggested siRNA concentrations -
(reply: 1)
Invitrogen's Negative control siRNA -
(reply: 3)
Dissolve siRNA in non-RNase free buffer -
(reply: 1)
Optimization of siRNA experiments -
(reply: 1)
Can I reconstitute ds siRNA in DW, - instead of 100 mM KOAC-20 mM HEPES?
(reply: 3)
siRNA in organotypic cultures - siRNA-mediated gene silencing in vitro and in vivo
(reply: 6)
siRNA vs. miRNA -
(reply: 5)
test the knockdown effficiency with siRNA before transfection with lentivirus? -
(reply: 1)
siRNA for retinal pigment epithelial cells -
(reply: 1)
RNAi of two different genes -
(reply: 4)
Can RNAi be used for silencing of non-coding RNAs? -
(reply: 5)
Combinatorial RNAi - double gene knockdown -
(reply: 4)
siRNA transfection in endothelial cells -
(reply: 3)
double siRNA knockdown? - procedure for simultaneous silencing
(reply: 17)
RNAi side effects -
(reply: 6)
RNAi vector construction - Has anybody done it?
(reply: 2)
siRNA transfection -
(reply: 4)
RNAi rescue by ectopic expression -
(reply: 2)
validated siRNA not working plz help -
(reply: 4)
gene duplication - affected by siRNA?
(reply: 1)
RNAi - possibilities and applications -
(reply: 3)
siRNA in primary lymphocyte B - Which reagent do you recomend?
(reply: 2)
Control RNAi ! -
(reply: 2)
siRNA "knock-out"/transfection "knock-in" -
(reply: 10)
Good Review of RNAi -
(reply: 2)
siRNA knock down did not work - protein expression knock down by siRNA
(reply: 4)
newbie questions on RNAi / microRNAs -
(reply: 3)
Scramble siRNA shows increase in gene expression by Taqman RTPCR -
(reply: 1)
siRNA positive control -
(reply: 3)
detect siRNA derived from shRNA construct -
(reply: 5)
SiRNA in MIN6 cells, nucleofection -
(reply: 1)
siRNA stable cell lines -
(reply: 6)
siRNA transfection -
(reply: 5)
siRNA causing no protein knockdown -
(reply: 1)
siRNA transfection -
(reply: 3)
sirna half life - time of effect
(reply: 2)
Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S
(reply: 1)
mRNA expression low/no effect of the siRNA -
(reply: 1)
Cytoplasmic granulation after siRNA transfection -
(reply: 3)
siRNA vs miRNA - why multiple terms for literally the same process? -
(reply: 3)
RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down?
(reply: 4)
siRNA transfection in suspended cell lines MV4-11, THP-1 -
(reply: 1)
siRNA transfection problem -
(reply: 4)
siRNA transfection reagent -
(reply: 9)
beginners guide to stable RNAi transfection -
(reply: 1)
miRNA targets in comparison to siRNA targets -
(reply: 2)
Basic question about siRNA, shRNA..etc - What's the difference?
(reply: 5)
Sources for vector-based siRNA against kinases? -
(reply: 1)
Is RNAi patented? -
(reply: 4)
siRNA and mices - What is the best way to in vivo siRNA transfer
(reply: 6)
Time point for reduction of protein levels after RNAi -
(reply: 1)
Knocking down miRNA using siRNA -
(reply: 5)
shRNA/siRNA which loop sequence? -
(reply: 2)
SiRNA and GFP transfection MCF7 cells -
(reply: 4)
Off-target effects of RNAi -
(reply: 4)
siRNA delivery in non-dividing cells -
(reply: 8)
How to trace siRNA in cells -
(reply: 4)
help with choosing model system for optimization of transfection of siRNA - and best deal for positive control?
(reply: 5)
shelf life of siRNA -
(reply: 1)
Confirming SiRNA Knockdown -
(reply: 6)
Increased transcripts after RNAi -
(reply: 10)
siRNA transfection of MIN6 cells -
(reply: 2)
siRNA purification -
(reply: 2)
Effect of scrambled siRNA on gene expression -
(reply: 5)
Has anyone seen target mRNA increasing after RNAi? -
(reply: 7)
Position of RNAi target on mRNA - How important is the location
(reply: 2)
RNAi in yeast? -
(reply: 3)
siRNA in non-adherent cell lines? - Tips please
(reply: 4)
How long DNA and siRNA stay in transfected cells -
(reply: 2)
how to start for effective RNAi screening--scale up question -
(reply: 2)
How to know siRNA transfection efficiency? -
(reply: 4)
about siRNA and shRNA - RNAi Vector
(reply: 2)
siRNA to shRNA problem -
(reply: 7)
Knockdown protein expression using rnai -
(reply: 4)
How to choose siRNA when BLAST results show high similarity with other gene -
(reply: 5)
RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR
(reply: 2)
siRNA causing mRNA knockdown but no protein knockdown! -
(reply: 4)
RNAi in a sense/antisense transcript system -
(reply: 3)
Help me ! I want to do RNAi -
(reply: 1)
RNAi negative controls -
(reply: 2)
nonspecific cellular responses - vector system vs synthetic siRNA -
(reply: 1)
Quantitative gene knock down with siRNA -
(reply: 1)
Use of dual promoter for siRNA -
(reply: 6)
How to choose the best optimum RNAi kit? -
(reply: 1)
Designing RNAi construct for adult Drosophila -
(reply: 1)
siRNA /shRNA agains EGFP -
(reply: 4)
siRNA transfetion in MEL (murine erythroleukemia) cell -
(reply: 3)
Is there article about lymphoma therapy with RNAi? -
(reply: 1)
how to convert RNA-based siRNA to DNA-based siRNA? -
(reply: 1)
Designing siRNA expression vectors -
(reply: 2)
what's the best system to get siRNA into a cell? suggestions -
(reply: 1)
RNAi knockdown versus DNA knock-out -
(reply: 2)
siRNA finder -
(reply: 3)
siRNA in Drosophila S2 cells? -
(reply: 4)
methods to 'quantify' morphology change in siRNA treated cells -
(reply: 1)
siRNA northern -
(reply: 1)
siRNA versus shRNA -
(reply: 4)
siRNA transfection of primary lymphocytes -
(reply: 1)
siRNA to shRNA -
(reply: 7)
extraction of endogeneous mirna and sirna -
(reply: 6)
Can I target non-coding sequence by siRNA -
(reply: 6)
Does siRNA transfection dependents on cell type -
(reply: 2)
transfecting siRNA and a plasmid -
(reply: 4)
siRNA - augmentation instead of knockdown - siRNA - augmentation instead of knockdown
(reply: 2)
siRNA transfection into 3T3-L1 -
(reply: 3)
siRNA for Akt1 - use of commercial siRNA to down-regulate Akt
(reply: 4)
How many is the limit? - What is the numerical limit to RNAi and others?
(reply: 2)
What is the different between microRNA and siRNA? -
(reply: 4)
How to transfect siRNA into suspension cell lines? -
(reply: 3)
RNAi in Jurkat cells -
(reply: 3)
Different siRNA transfection agents - Have enyone tested them???
(reply: 44)
chemical synthesized siRNA and rapidly dividing cells -
(reply: 2)
siRNA in vivo?? -
(reply: 7)
several questions for siRNA target BLAST -
(reply: 4)
RNAi vector for stable expression -
(reply: 4)
concentrate siRNA -
(reply: 1)
stable transfection with siRNA expression vector -
(reply: 6)
siRNA target selection problem -
(reply: 11)