siRNA kit - expensive (Sep/24/2005 )
The siRNA kit from Qiagen is so expensive !!!
My boss wishes to use siRNA but he does not have the money. He was disappointed when I told him the price for the kit.
If I got a PhD scholarship, I may be able to donate the scholarship to fund the siRNA study. Unfortunately I am just a self-funded PhD student and it will be hard for me to donate my saving (for paying the parking fee and petrol so that I can come to work at 6-7am and left at 8-9pm).
Do anyone know how to perform siRNA without using the expensive kit?
I don't know what kit from Qiagen you are referring to and what are included.
There are at least 3 ways of making siRNA for transfection.
- Chemically synthetic siRNA - around $300 for each siRNA duplex, time-saving, reliable
- Hairpin siRNA which is cloned from DNA oligos - You need to buy the vector, oligos, do ligation, transformation, screening, plasmid preparation etc, error prone, time consuming
- PCR expression cassette - seems to be the most economic way of producing siRNA, but the quality of such siRNA may be not as good as synthetic siRNA.
Ambion website provides a very nice comparison table comparing many aspects of different ways of making siRNA http://www.ambion.com/techlib/tn/103/2.html
If you only have a few target genes, I would suggest that you go for synthetic siRNA. What you will need are gene specific siRNA, a control siRNA, transfection reagent such as lipofectamine 2000 from Invitrogen. The total cost may be well above $500.
If you can find someone who is willing to share his/her existing cloned hsRNA vector targeting your gene (check the literature), that will save you lot of money and time.