siRNA transfection of primary lymphocytes - (Jun/22/2005 )
Is anyone tried to transfect primary lymphocytes with siRNA. Is electroporation the only possible method. Thanks for helping
I have transfected siRNA on primary lymphocytes using liposome reagents, DEAD-dextran and electroporation. DEAE and electroporation caused huge cell loss (~40-60%) while certain liposome reagent did give me desirable efficiency with low toxicity, the best one in my hands is called RNAicarrier (Epoch biolabs). Also, primary lymphocytes are very fragile and sensitive, be careful and good luck.