siRNA and Hybond XL - any one use them? (Dec/08/2006 )
I'm trying to detect by northern blot the siRNA transcribed in my cells. For that purpose, i'm using Hybond XL membrane which was purchased by my lab already, but no success...
i was searching google for articles webpages or anything that shows a northern showing siRNA with this membrane but can't find anything.
here is the question :
does anyone in the forum do siRNA transcription detection?
does anyone use amersham XL membrane for that?
i've missed surely sthg and if someone can provide me only the ref that would maybe save me lot of time...
I recommend Hybond-N+ for siRNA, I have try Hybond-NX once, but no satisfied signal