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how to detect double strand siRNA - (Aug/16/2007 )

How to detect double strand siRNAs (product of siRNA/shRNA expression construct)


normally, a sense probe is able to detect by northern both siRNA from sh precursor and the guide strand too.
special rtpcr kits are emerging now.


Has anyone perform such work? I just wondering whether the up stand siRNA showing similar abundance as low strand siRNA