how to detect double strand siRNA - (Aug/16/2007 )
How to detect double strand siRNAs (product of siRNA/shRNA expression construct)
-rye-
normally, a sense probe is able to detect by northern both siRNA from sh precursor and the guide strand too.
special rtpcr kits are emerging now.
-fred_33-
Has anyone perform such work? I just wondering whether the up stand siRNA showing similar abundance as low strand siRNA
-rye-