U6, H1 siRNA - (Dec/11/2006 )
hi I have a question about shRNA promoter
what is the difference between H1 and U6.
is the polyT added sequence in 3' of the oligo are specific for H1 or U6?
poly T sequences after either H1 U6 or 7SK are terminal signals for the polymerase III transcription machinery.
Both promoters you've cited are efficient for siRNA transcription.