Double SiRNA trasfection - (Nov/05/2008 )
i want to determine the effect of knocking down 2 genes simultaneously by sirna(smart pool from dharmacon). i think its practically possible, but what controls should i take and how shall i monitor the transfection efficiency?
For a start, see the discussion pinned at the top of the contents page for this siRNA, microRNA and RNAi forum, titled "double siRNA knockdown?". That doesn't specifically address your question, but provides some good background discussion.