Has anybody ever used Dharmacon ON-TARGETplus SMARTpool siRNA? - (Feb/20/2008 )
I am wodering if anybody has experience in using Dharmacon ON-TARGETplus SMARTpool siRNA. I have trouble using Ambion siRNA and I may switch to Dharmacon siRNA. Thanks for sharing.
I have used the ON-TARGETplus SMARTpool siRNA against MCM5. This is an extremely stable protein and was concerned that I wouldn't be able to get an efficient knockdown but was pleasantly surprized to see that I could obtain a 90% (or better) loss of protein expression. I was very pleased at the ease and efficiency of this product and would recommend this company for siRNA needs.
Hello,
Glad to hear that. What transfection reagent have you used for transfceting the siRNA? Thanks.
dc11
Glad to hear that. What transfection reagent have you used for transfceting the siRNA? Thanks.
dc11
Yep am using from the same company and gettin a good knockdown . I also use the dharmafect reagent . I would recommend that
I have used them. Great results! 90% Knockdown between 24 and 96 hours.
They provide their own transfection reagents (i think they were called 1, 2 3 & 4 , you check out all 4 using their trial kit and use the one that works best on your particular cells. I am not sure they still sell this trial kit. But by now, they should know which one works best for which cells, just call their techrep/
How was the toxicity for dharmafect? acceptable?
acceptable.
They have good control siRNAs, fluorescent tagged siRNAs etc. tagged ones are great to determine which cells are actually transfected.
I however prefer viral siRNAs these days for their prolonged knockdown capability. Some cellular phenotypes would only emerge when gene is knocked-down for a long time--especially if you have particularly stable protein.