siRNA transfection - (Jun/21/2007 )
Hi,
I am working on primary mouse macrophages. I need to transfect siRNAs into them. Could anyone help me with regard to the protocol and suggest the ideal transfection agent I need to use?
Thanks
-sds_doc-
QUOTE (sds_doc @ Jun 21 2007, 01:16 PM)
Hi,
I am working on primary mouse macrophages. I need to transfect siRNAs into them. Could anyone help me with regard to the protocol and suggest the ideal transfection agent I need to use?
Thanks
I am working on primary mouse macrophages. I need to transfect siRNAs into them. Could anyone help me with regard to the protocol and suggest the ideal transfection agent I need to use?
Thanks
Hi !
I have never tried to transfect primary macrophages, but I have ever transfected SiRNA into raw264, which is a macrophages cell line with PolyMag from OZBiosciences and it works. PolyMag is a transfection agent based on the magnetofection technology developed by this supplier. You can take a look on their website for more information.
Hope that helps.
-loli13-
Hi Loli,
Thanks but I am working on mouse peritoneal macrophages that are more sensitive to transfection agents than that which can be used on RAW cell line....so I am still trying to figure out what can be used as an optimal transfection agent...any suggestions on that?
Thanks for your reply
-sds_doc-
Primary macrophages are very tough to transfect. You may try electroporation or lentiviral vector.
-genehunter-1-