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QPCR Related Discussions
  1. qPCR problems with chIP samples - (reply: 2)
  2. qPCR delta delta CT calculation - (reply: 1)
  3. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
  4. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  5. normalizing between qPCR runs - (reply: 2)
  6. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
  7. qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
  8. qPCR troubles - (reply: 5)
  9. qPCR 96 well plate - (reply: 2)
  10. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
  11. qRT-PCR primer design - (reply: 1)
  12. QPCR data anlaysis - (reply: 4)
  13. Problems with second use of diluted cDNA in qPCR - (reply: 4)
  14. qRT-PCR - data analysis - methods that DO NOT use a calibrator ! (reply: 3)
  15. First strand cDNA synthesis for qRT-PCR - (reply: 1)
  16. qRT-PCR control - (reply: 1)
  17. Difference between semi-q and qPCR - (reply: 2)
  18. Multichannel Pipette for qPCR plates - Effective manual pipetting of qPCR plates (reply: 1)
  19. Stratagene MX4000 qPCR plates to give away - UK only & preferably pick up in Cambridge (reply: 5)
  20. Magnetic beads in a QPCR rxn - (reply: 1)
  21. qPCR internal control - (reply: 3)
  22. unsolved qPCR problem! - (reply: 1)
  23. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
  24. qRT-PCR: how to average multiple runs? - (reply: 1)
  25. QPCR - (reply: 9)
  26. QPCR - (reply: 2)
  27. qPCR - (reply: 1)
  28. New to qPCR - (reply: 2)
  29. Low Ct value in ChIP qPCR - (reply: 4)
  30. real-time PCR problem - (reply: 1)
  31. Real-time PCR for mRNA stability - (reply: 2)
  32. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
  33. qRT-PCR reproducibility of triplicates? - (reply: 6)
  34. miRNA real-time using Sybr green - (reply: 9)
  35. How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
  36. qPCR standard curve problem - (reply: 1)
  37. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
  38. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  39. Problem with qPCR of ChIP input - (reply: 12)
  40. Using real-time to differentiate between SNPs - (reply: 5)
  41. Bad primer efficiency during multiplex qPCR - (reply: 1)
  42. What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
  43. qRT-PCR first timer, controls? - (reply: 9)
  44. ChIP and qPCR - ChIP and qPCR (reply: 4)
  45. Ki67 IHC vs Real-time PCR - studying proliferation (reply: 1)
  46. Fluorescence level in real-time - (reply: 2)
  47. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
  48. miRNA array data and QPCR data correlation - (reply: 6)
  49. need help on microRNA real-time PCR - (reply: 6)
  50. standard curve method for relative qPCR quantitation - (reply: 2)
  51. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  52. Primer concentration is quantitative Real-time PCR - (reply: 1)
  53. choosing real-time PCR machine - (reply: 13)
  54. Statistics help with real-time relative quantification (RQ) values - (reply: 2)
  55. Real-Time PCR and Housekeeping gene - (reply: 9)
  56. ChIP and qRT-PCR - (reply: 1)
  57. Controls for real-time PCR... again - (reply: 7)
  58. ChIP qPCR data analysis? - (reply: 9)
  59. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  60. absolute qPCR problem - (reply: 1)
  61. Simple QPCR question? - (reply: 1)
  62. Detection of LNA miRNA knock-down by qPCR? - (reply: 5)
  63. Taqman qpcr sensitivity - (reply: 1)
  64. Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
  65. RT-PCR Vs qRT-PCR help please! - (reply: 4)
  66. SYBR green concentration for qPCR - (reply: 2)
  67. PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
  68. qrt-pcr on bacterial transcripts - (reply: 1)
  69. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  70. qPCR on low to non-existant template - (reply: 4)
  71. elp with qPCR standard curve - (reply: 5)
  72. HELP with qPCR results - (reply: 3)
  73. QPCR primer Pairs - (reply: 1)
  74. qPCR Exp. Design - Reruning Exp. Control Samples - (reply: 3)
  75. Statistics: real-time RT-PCR - (reply: 4)
  76. QPCR versus regular PCR for Chip - (reply: 2)
  77. what is meaning of true enrichment for chip qPCR? - (reply: 5)
  78. MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
  79. Help for Real-time PCR equipment choice - (reply: 4)
  80. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
  81. qRT-PCR help! - (reply: 2)
  82. qRT-PCR and Northern blot - Is miRNA Northern blot necessary? (reply: 2)
  83. gene-specific Primer for qRT-PCR - (reply: 2)
  84. No signal in Real-time but in normal PCR?! - (reply: 2)
  85. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
  86. Real-time PCR low efficiency - (reply: 1)
  87. qPCR databases - (reply: 1)
  88. qPCR and plasmid positive controls - Why use plasmid positive controls? Are they the best? If so, are there (reply: 1)
  89. QPcr for alternatively spliced products - (reply: 4)
  90. What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
  91. qPCR primer efficiancy - (reply: 2)
  92. ChIP and qPCR calculation ? - (reply: 8)
  93. Touchdown conditions for qPCR - (reply: 1)
  94. QPCR Quantitation of copy number - (reply: 1)
  95. ELISA in a real-time PCR plate - possible? (reply: 3)
  96. genomic DNA for qPCR quantification - target sequence? - (reply: 1)
  97. looking for a website for a newbie to learn qPCR - (reply: 3)
  98. qPCR for microRNAs - minimal primer length? (reply: 6)
  99. Real-Time MSP - (reply: 1)
  100. preference for region of primer design for qRT-PCR - (reply: 1)
  101. qPCR housekeepers - primers or probes? - (reply: 1)
  102. QPCR Oligo Database of Pathogens - (reply: 1)
  103. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  104. Free sample from Takara. - All kind of polymerase for PCR and real-time PCR. (reply: 7)
  105. Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
  106. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
  107. Real-time PCR optimization - (reply: 8)
  108. Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
  109. Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
  110. Real-time PCR....Making sense of it all? - (reply: 3)
  111. Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
  112. QPCR with MSP - (reply: 3)
  113. Real-time PCR to analyze DNA methylation - (reply: 3)
  114. qPCR using random primers - (reply: 4)
  115. Verification of real-time amplification specificity - (reply: 1)
  116. Problems with RNA extraction or QPCR for AI - (reply: 2)
  117. miRNA QPCR advice - taqman (reply: 3)
  118. No-RT control in qRT-PCR - (reply: 5)
  119. Longest Amplicon Using Real-Time PCR? - (reply: 1)
  120. cDNA for real-time PCR - (reply: 3)
  121. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  122. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
  123. normalization for real-time PCR - (reply: 4)
  124. One-step versus two-step qRT-PCR - (reply: 5)
  125. primer for real-time and reverse transcriptase PCR - (reply: 1)
  126. Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
  127. qPCR vs RNA protection assay - (reply: 1)
  128. Plasmid for qPCR standard curve - (reply: 3)
  129. Can you use Picogreen in a QPCR instrument? - (reply: 1)
  130. Real-Time PCR Question - (reply: 1)
  131. Target Amplicon Length for real-time PCR - (reply: 4)
  132. Real-Time PCR/Melting Curve Question - (reply: 3)
  133. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  134. Real-Time PCR Amplicon Question - (reply: 2)
  135. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
  136. Diluting your cDNA for qRT-PCR? - (reply: 5)
  137. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
  138. Help! Opticon real-time machines thinks I only want to do 1 cycle! - (reply: 3)
  139. What about the BIO-RAD's real-time machine? - (reply: 2)
  140. How can I prepare standard DNA for QPCR - (reply: 3)
  141. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
  142. qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
  143. Standard Curve Issues with ABI 7500 qPCR - (reply: 4)
  144. Pre-Preparation of qPCR plates - Pros and Cons (reply: 4)
  145. ChIP qPCR analysis - (reply: 2)
  146. housekeeping gene in real-time PCR - (reply: 2)
  147. Trouble with real-time PCR standard curve - (reply: 4)
  148. 2 melting peaks in real-time PCR - (reply: 1)
  149. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
  150. Relative Quantification in real-time RT-PCR - (reply: 5)
  151. mirVana qRT-PCR with the Roche 1.5 LightCycler - (reply: 1)
  152. Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
  153. NTC's in RT or just qPCR? - (reply: 3)
  154. Can't repeat a perfect miRNA real-time standard curve - (reply: 2)
  155. qPCR Efficiencies over 100% - Looking for references (reply: 1)
  156. real-time PCR for quantitation of MSP - (reply: 3)
  157. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  158. Small amounts of RNA for qRT-PCR - (reply: 7)
  159. qPCR Standard control - plasmid quantify (reply: 3)
  160. Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
  161. how long should qPCR product be? - 150~250bp? (reply: 2)
  162. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
  163. Strange thing about RNA concentration and real-time melting curve - (reply: 4)
  164. random primers for qPCR - (reply: 3)
  165. QPCR, where to start? - optimizing primer and template (reply: 5)
  166. qPCR standards - (reply: 1)
  167. Taqman QPCR help - amplification of FAM right from the 0 cycle (reply: 5)
  168. QPCR Copy number - (reply: 2)
  169. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
  170. qPCR detection for single nucleotide substitution - (reply: 5)
  171. Best product size for real-time RT-PCR - (reply: 4)
  172. Which housekeeping genes for qRT-PCR control? - (reply: 17)
  173. Replication of qRT-PCR experiments - (reply: 5)
  174. generation of gDNA Std curves in qPCR - ABI 7300 qPCR (reply: 1)
  175. Minimum amount of RNA for real-time PCR - (reply: 4)
  176. is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
  177. genomic dna in real-time pcr - (reply: 3)
  178. What's the function of UNG in qPCR? - (reply: 6)
  179. What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
  180. ChIP quantification (qPCR) - (reply: 2)
  181. Not enough cDNA for qRT-PCR - how to increase cDNA yield or save cDNA? - (reply: 12)
  182. Self-made qRT-PCR enzyme-/mastermix? - (reply: 8)
  183. Home-made Sybr green mix for real-time PCR - (reply: 1)
  184. Should I dilute cDNA for real-time PCR? - (reply: 3)
  185. Real-time PCR problems - RT_PCR (reply: 7)
  186. strange contamination in real-time PCR in negative control? - (reply: 1)
  187. Problem with QPCR - control gene does not amplify - (reply: 2)
  188. qPCR for HKG and traditional PCR for GOI - (reply: 1)
  189. Smeary bands in real-time RT-PCR - (reply: 7)
  190. Touch down real-time PCR? - (reply: 1)
  191. real-time slopes - (reply: 4)
  192. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  193. 1st time real-time PCR questions - (reply: 1)
  194. real-time pcr optimization problem - (reply: 4)
  195. Optimal size for qRt-PCR - (reply: 3)
  196. Primer Dimer problems in real-time PCR - (reply: 14)
  197. Has anyone used 8E5 cells as standard for HIV qPCR? - HIV standards (reply: 1)
  198. some qPCR questions (naive ones included)... - first pitfals etc... (reply: 8)
  199. designing QRT-PCR Primers - (reply: 11)
  200. Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
  201. Another QPCR problem. Please help - (reply: 5)
  202. Trouble with standard curves for real-time PCR - (reply: 6)
  203. Real-time PCR machines for DNA quantitation - (reply: 5)
  204. qPCR after ChIP - lack of reproducibility - (reply: 9)
  205. Use of ROX in qPCR - (reply: 8)
  206. strange QPCR amplificaton plot - (reply: 3)
  207. total RNA from BSE infected WBC for microarray and qPCR - (reply: 2)
  208. Mg curve for real-time PCR - Strange result (reply: 5)
  209. Northern vs qRT-PCR for expression quantitation - (reply: 6)
  210. Real-time PCR probe degradation - when do they degrades? (reply: 4)
  211. My SYBRgreen qPCR stopped working - (reply: 3)
  212. AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
  213. real-time thresholds - purpose? - (reply: 1)
  214. Real-time PCR - contamination or not? - (reply: 6)
  215. real-time PCR - Multiple melting curve peaks (reply: 18)
  216. Real-time PCR questions (Mx3000P) - Need some help with interpretation (reply: 7)
  217. QPCR books - (reply: 1)
  218. qRT-PCR data analysis - (reply: 1)
  219. qPCR and siRNA - (reply: 2)
  220. mastermix for Real-time PCR - (reply: 2)
  221. Real-Time qRT-PCR standard curves... efficiency is too high! - (reply: 19)
  222. qPCR standard curves - how can I keep the quality good? (reply: 6)
  223. Construct DNA plasmid for QPCR - (reply: 4)
  224. real-time PCR - (reply: 5)
  225. Real-time PCR amplification curve up and down - (reply: 3)
  226. Which RT primer is better for qPCR - (reply: 2)
  227. Do I have amplication of RNA by qPCR? - (reply: 7)
  228. melt curves for SYBR Green real-time PCR driving me nuts!! - melt curves real-time PCR (reply: 6)
  229. real-time RT-PCR one cell - (reply: 2)
  230. real-time pcr plasmid standards - (reply: 4)
  231. QRT-PCR - (reply: 4)
  232. contamination in real-time PCR - (reply: 32)
  233. Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
  234. contamination in real-time pcr negative control - (reply: 12)
  235. Real-time PCR problems - (reply: 3)
  236. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
  237. Real-time PCR and high Ct value - (reply: 1)
  238. product size for real-time PCR primer - (reply: 3)
  239. Efficiency Real-Time PCR - Real-time PCR (reply: 3)
  240. Real-time quantification in Chromatin IP - (reply: 2)
  241. qPCR on genomic DNA - (reply: 2)
  242. Real-Time PCR - No plateau! (reply: 1)
  243. qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
  244. does anyone know real-time methyaltion pcr? - (reply: 1)
  245. real-time PCR - How to design primers (reply: 2)
  246. About Q-PCR(real-time PCR) - COMPARE (reply: 1)
  247. QRT-PCR result analysis - relative transcript level? (reply: 3)
  248. Familiar with MX3000P by Stratagene?? - qPCR (reply: 1)
  249. Probes for Real-Time PCR - (reply: 3)