Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Transfection Related Discussions
  1. transfection of suspended CHO cells - (reply: 2)
  2. Hek293 transfection help!!!! - (reply: 1)
  3. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  4. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  5. Help with HUVEC cell transfection - (reply: 3)
  6. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  7. GFP interference in dual luciferase assay? - (reply: 2)
  8. preparing a vector for transfection - (reply: 2)
  9. Easy to transfect primary cell line? - (reply: 1)
  10. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  11. transfection of cells with two fluorescent reporters - (reply: 1)
  12. Stably transfected cells start dying - (reply: 6)
  13. not successful with transfection - (reply: 5)
  14. Transient transfection in huvec - (reply: 1)
  15. Blasticidin dose for Transfected - (reply: 1)
  16. Transfection problem - (reply: 6)
  17. Transfection media change - (reply: 1)
  18. Transfection of SH-SY5Y cells - (reply: 4)
  19. help for chip assay and luciferase assy - (reply: 1)
  20. Transfection help - (reply: 4)
  21. Viral RNA in total RNA eluate for transfection - (reply: 1)
  22. Stability of transient shRNA transfection - (reply: 1)
  23. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  24. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  25. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  26. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  27. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  28. Transfection Enhancer Efficiency - (reply: 1)
  29. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
  30. CHO-K1 transfection problem - (reply: 2)
  31. Using pBABE plasmids for regular transfection - (reply: 1)
  32. Subsequent DNA and siRNA transfection - (reply: 9)
  33. need help for transfection to synchronized cells - (reply: 4)
  34. 293t transfected with prtTA inducible vex - (reply: 5)
  35. No expression in stable transfected cell line - (reply: 2)
  36. Dead cells after transfection - (reply: 9)
  37. preparation of cells for FACS analysis after transfection - (reply: 4)
  38. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
  39. Transient transfection with large plasmid - (reply: 3)
  40. transfection using lipofectamine 2000 - (reply: 3)
  41. Determining phosphorylation status of a protein by transfection - (reply: 5)
  42. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  43. PGL-3 Luciferase Inactivity - (reply: 2)
  44. What is the optimal time for transfection efficiency measuring? - (reply: 1)
  45. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
  46. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  47. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  48. Transfection but no expression - (reply: 4)
  49. Cells Detach After Transfection - (reply: 1)
  50. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  51. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  52. Transfection methods for human ESCS of iPSCs - (reply: 1)
  53. Simultaneous RNA and DNA transfection? - (reply: 2)
  54. death of transfected cells after first passage in G418 medium - (reply: 1)
  55. transfection with lipofectamine 2000 advice - (reply: 4)
  56. clonogenic assay for shRNA transfected cells - (reply: 3)
  57. Luciferase fusion protein? - (reply: 1)
  58. How did they do this? Microinjection? Transfection? - (reply: 2)
  59. Luciferase assay problem - (reply: 2)
  60. Transient transfection of linearized plasmids - (reply: 3)
  61. HUVEC plasmid transfection challenges - (reply: 2)
  62. Magnet assisted transfection - (reply: 1)
  63. Trouble with shRNA transfection - (reply: 2)
  64. Transient Transfection Controls (Gateway Technology) - (reply: 4)
  65. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  66. To small amount of cells used for shRNA transfection - (reply: 1)
  67. Dual Luciferase Assay - (reply: 1)
  68. Determining the amount of plasmid transfected - (reply: 1)
  69. Can I scale up at transfection, after determining the drug concentration via kil - (reply: 2)
  70. Luciferase assay - extreme variation in RLU - (reply: 1)
  71. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  72. Insect cells transfection reagent - (reply: 1)
  73. primary valve interstitial cells transfection - (reply: 1)
  74. Problem in transfection for producing lentivirus - (reply: 4)
  75. Transfection of a non human gene into human cell line - (reply: 1)
  76. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
  77. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  78. Recommendation on siRNA transfection reagents - (reply: 2)
  79. lipofectamine 2000:DNA complex stability - (reply: 2)
  80. Transfection reagent stored at -20C - (reply: 2)
  81. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  82. Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 - (reply: 1)
  83. transient transfection problem using ME180 cells - (reply: 1)
  84. Linearising plasmid before transfection? - (reply: 2)
  85. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  86. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  87. Fugene transfections - (reply: 1)
  88. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  89. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  90. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  91. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  92. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
  93. Dual Luciferase assay data analysis issues - (reply: 4)
  94. HEK 293T Transfection problem - (reply: 4)
  95. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  96. Luciferase: too much pbs - (reply: 4)
  97. Transfection Rate - (reply: 1)
  98. Calcium Phosphate transfection - storage? - (reply: 1)
  99. Calcium chloride plasmid transfection - (reply: 1)
  100. virus transduction vs plasmid transfection - (reply: 4)
  101. Luciferase Assay Weird Result: TK-Renilla Changing More than SV40 Firefly - (reply: 1)
  102. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  103. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  104. Stable transfection - (reply: 2)
  105. Luciferase assay-normalisation - (reply: 2)
  106. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  107. Transfection with T-PRO reagent - Any tips? - (reply: 1)
  108. Cotransient Transfections DNA Requirements - (reply: 3)
  109. Help with Dual luciferase assay - (reply: 1)
  110. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  111. Luciferase assay - expected range of raw reading values - (reply: 1)
  112. Calculating siRNA Transfection Efficiency - (reply: 3)
  113. LENTIVIRUS transfection efficiency - (reply: 1)
  114. Transfection problem after transformation! - (reply: 5)
  115. Transfection - (reply: 1)
  116. Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
  117. Measure transfection effiicency - referee's question - (reply: 1)
  118. cells die after transfection - (reply: 1)
  119. What is cotransfection in molecular cell biology? - (reply: 1)
  120. stable transfection - (reply: 1)
  121. Chloroquine use in transfection!!! - (reply: 2)
  122. Problems with double transfections -cells die - (reply: 7)
  123. NIH 3T3 Stable Line Transfection - (reply: 1)
  124. GFP and luciferase measurements - (reply: 3)
  125. extract plasmids from transfected eukaryotic cells - (reply: 6)
  126. identification of the transfection efficiency - (reply: 4)
  127. stable transfection suspension cultures - (reply: 1)
  128. problem with transient transfection - (reply: 6)
  129. Problem in Stable transfection - (reply: 3)
  130. Transfection & Cilia help! - (reply: 3)
  131. Storage of transfected PC12 cells - (reply: 1)
  132. Transfection - Optimising Transfection Protocol (reply: 2)
  133. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  134. problem with siRNA transfection of HEK cells - (reply: 5)
  135. Viral transfection to neurosphere in vitro - (reply: 1)
  136. What transcription factors are involved in transient transfection - (reply: 1)
  137. NMDA Transfection in 293 Cells - (reply: 1)
  138. Lipofectamine and transfection - (reply: 2)
  139. problems at normalizing luciferase assay data - (reply: 1)
  140. dual luciferase data analysis - (reply: 2)
  141. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
  142. Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
  143. Big plasmids and Transfection - (reply: 3)
  144. CaMKII alpha promoter and cell tranfection - (reply: 1)
  145. How to explain this luciferase result?! - (reply: 2)
  146. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  147. MDA-MB-231 cell transfection - (reply: 4)
  148. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  149. why these guys use T7 instead of CMV? - for transfection (reply: 2)
  150. Luciferase assay - (reply: 2)
  151. NIH3T3 Transfection - (reply: 1)
  152. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  153. siRNA transfection with lipofectamine 2000 - (reply: 1)
  154. Will transfected plasmids induce cytokine production? - (reply: 1)
  155. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  156. Transfection in insect cell lines?? - (reply: 1)
  157. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  158. ShRNA transfection - (reply: 1)
  159. Luciferase Assay Stim. Concentration? - (reply: 1)
  160. miRNA/siRNA transfection doubts - (reply: 2)
  161. Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
  162. TE buffer in PEI transfection - (reply: 1)
  163. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
  164. Virus with serum - viral transfection (reply: 1)
  165. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  166. Neuron transfection method-need the best protocol - (reply: 4)
  167. 3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
  168. transfection or infection - difference? (reply: 1)
  169. DharmaFECT transfection reagent - (reply: 1)
  170. stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
  171. luciferase GPCR assays - (reply: 1)
  172. Problems with transfection in human cells - (reply: 3)
  173. Preferences for MEF transfections - (reply: 1)
  174. CaPO4 transfection - (reply: 1)
  175. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  176. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  177. 293 Transfection Troubleshoot ! - (reply: 2)
  178. Luciferase and cell responsiveness - (reply: 3)
  179. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  180. Transfection Problem - (reply: 3)
  181. 293T transfection - (reply: 1)
  182. lenti virus transfected cell dies... - (reply: 4)
  183. how to compensate the cell death during transfection by electroporation - (reply: 2)
  184. how to minimize the variation among the replicas - transcient transfection (reply: 4)
  185. Transfection - (reply: 1)
  186. normalization of dual luciferase assay - (reply: 4)
  187. dual luciferase reporter?? - (reply: 2)
  188. Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
  189. Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
  190. Methylation and luciferase reporter query - (reply: 4)
  191. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  192. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  193. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  194. explain Transient transfection - (reply: 1)
  195. Help! low efficiency of transfection - (reply: 2)
  196. pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
  197. Stable transfection selection - (reply: 3)
  198. transfection is not working - (reply: 4)
  199. Transfection didnt work after repeating it. - (reply: 11)
  200. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  201. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  202. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  203. suspension cell transient transfection - (reply: 10)
  204. After Transfection - (reply: 1)
  205. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  206. Early transfection - (reply: 6)
  207. Plant cell transfection - (reply: 1)
  208. luciferase - (reply: 1)
  209. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  210. Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
  211. Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
  212. Basic questions about Transfection - About Lipofectamine (reply: 3)
  213. GFP and Luciferase Cell Line - (reply: 2)
  214. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  215. Luciferase signal - (reply: 5)
  216. Transfection into eukaryotic cells - (reply: 1)
  217. Lentivirus Production: Problems / Transfection Method - (reply: 4)
  218. Normal 96-well compatible for luciferase assay? - (reply: 3)
  219. Transfection - (reply: 1)
  220. Double transfection of siRNA - (reply: 2)
  221. NIH3t3 transfection optimization - (reply: 1)
  222. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  223. problem in plasmid transfection - (reply: 2)
  224. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  225. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  226. How to normalize my samples before I transfect my cells - (reply: 6)
  227. transfection efficiency - (reply: 1)
  228. transfection time - (reply: 2)
  229. problem in transfection - (reply: 4)
  230. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  231. transient transfection - (reply: 1)
  232. stable transfection problem - (reply: 1)
  233. Luciferase transfection controls - (reply: 3)
  234. checking transient transfection efficiency - (reply: 3)
  235. Immunofluorescence with transfected cells - (reply: 3)
  236. GFP constructs in Luciferase assay - (reply: 4)
  237. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  238. suspension cell transient transfection - (reply: 2)
  239. cell cryopreservation after siRNA transfection - (reply: 1)
  240. Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
  241. lipofectamine and PEI transfection of hela cells - (reply: 2)
  242. Hoe much media needed for transfection complex? - transfection (reply: 9)
  243. Neon Transfection System - (reply: 2)
  244. Transfection before plating? - (reply: 2)
  245. Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
  246. Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
  247. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  248. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  249. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  250. Dual Luciferase Assay - (reply: 3)
  251. Transfection - Plasmid Uptake Control - (reply: 2)
  252. Luciferase GL4 siRNA - (reply: 5)
  253. Two virus method for transfection - (reply: 1)
  254. luciferase assay - basic information on luciferase assay (reply: 3)
  255. plasmid purification kit for transfection - (reply: 2)
  256. best plasmid purification kit for transfection - (reply: 1)
  257. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  258. Transfection: How long until translation starts? - (reply: 2)
  259. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
  260. Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
  261. How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
  262. Tranfection cell density and overgrowth - (reply: 3)
  263. Double transfection questions - (reply: 2)
  264. U2OS transient transfection - (reply: 6)
  265. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  266. How good are CHO cells - in general and for transfection (reply: 1)
  267. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
  268. Promoter Cloning for Luciferase Assay - (reply: 3)
  269. siRNA and plasmid cotransfection - (reply: 1)
  270. Tranfection of HL 60 cells - (reply: 1)
  271. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
  272. Transfection of NIH/3T3 - (reply: 2)
  273. Transfection problems - (reply: 5)
  274. expression of SV40 in luciferase assay - (reply: 3)
  275. Minimum transfection incubation time - (reply: 2)
  276. Transfection - (reply: 7)
  277. about measuring luciferase activity in living cells - (reply: 8)
  278. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  279. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  280. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  281. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
  282. Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
  283. transient transfection - (reply: 2)
  284. Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
  285. HepG2 siRNA transfection - (reply: 1)
  286. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  287. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
  288. Max. length for 3'UTR in luciferase assay? - (reply: 1)
  289. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  290. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  291. Luciferase assay - (reply: 3)
  292. Transfection problem - milky media - (reply: 1)
  293. stable transfection problems - stable knockdown (reply: 3)
  294. Luciferase reporter induced by betagal vector?? - (reply: 1)
  295. HeLa tranfection- Dual luciferase Assay - (reply: 2)
  296. transfection in floating cells - (reply: 5)
  297. Transfection Expression Level Issues - (reply: 6)
  298. Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
  299. complicated transfection requires professional help - (reply: 3)
  300. transient transfection efficiency - (reply: 2)
  301. Filtration DNA before transfection? - (reply: 4)
  302. 293s, transfection and MTT assay - help! (reply: 3)
  303. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  304. pIRESneo for mammalian cells transfection - (reply: 4)
  305. How to transfect two plasmids in a cell - (reply: 10)
  306. SKBR3 transfection - (reply: 1)
  307. lipofectamine - (reply: 3)
  308. fibroblasts transfection - (reply: 1)
  309. Firefly Luciferase Assay - variation in FL reading (reply: 2)
  310. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  311. transfection - (reply: 1)
  312. transfection - (reply: 1)
  313. Heterogenous expresion of stably transfected gene - (reply: 4)
  314. Retroviral vector with luciferase - (reply: 1)
  315. Transfection efficiency determination - (reply: 4)
  316. Transfection contamination? - (reply: 12)
  317. Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
  318. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  319. stable transfectants - (reply: 6)
  320. Quick lipofectamine question - (reply: 7)
  321. Transfection AML-193 - (reply: 3)
  322. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
  323. Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
  324. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  325. Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
  326. Target gene regulation by transiently transfected PR - (reply: 4)
  327. Transfection of vectors - (reply: 8)
  328. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  329. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  330. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  331. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  332. Hygromycin selection - Selection 72 hours after transfection (reply: 3)
  333. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  334. stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
  335. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)