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Transfection Related Discussions
  1. Blasticidin dose for Transfected - (reply: 1)
  2. Transfection problem - (reply: 4)
  3. Transfection media change - (reply: 1)
  4. Transfection of SH-SY5Y cells - (reply: 4)
  5. Transfection help - (reply: 4)
  6. Viral RNA in total RNA eluate for transfection - (reply: 1)
  7. Stability of transient shRNA transfection - (reply: 1)
  8. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  9. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  10. Lentivirus transfection using RAW 264.7 cells - (reply: 1)
  11. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 4)
  12. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  13. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 3)
  14. CHO-K1 transfection problem - (reply: 1)
  15. Using pBABE plasmids for regular transfection - (reply: 1)
  16. Subsequent DNA and siRNA transfection - (reply: 8)
  17. need help for transfection to synchronized cells - (reply: 4)
  18. 293t transfected with prtTA inducible vex - (reply: 5)
  19. No expression in stable transfected cell line - (reply: 2)
  20. Dead cells after transfection - (reply: 8)
  21. preparation of cells for FACS analysis after transfection - (reply: 4)
  22. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
  23. Transient transfection with large plasmid - (reply: 3)
  24. transfection using lipofectamine 2000 - (reply: 3)
  25. Determining phosphorylation status of a protein by transfection - (reply: 5)
  26. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  27. PGL-3 Luciferase Inactivity - (reply: 2)
  28. What is the optimal time for transfection efficiency measuring? - (reply: 1)
  29. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
  30. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  31. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  32. Transfection but no expression - (reply: 4)
  33. Cells Detach After Transfection - (reply: 1)
  34. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  35. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  36. Transfection methods for human ESCS of iPSCs - (reply: 1)
  37. Simultaneous RNA and DNA transfection? - (reply: 2)
  38. death of transfected cells after first passage in G418 medium - (reply: 1)
  39. transfection with lipofectamine 2000 advice - (reply: 4)
  40. clonogenic assay for shRNA transfected cells - (reply: 3)
  41. Luciferase fusion protein? - (reply: 1)
  42. How did they do this? Microinjection? Transfection? - (reply: 2)
  43. Luciferase assay problem - (reply: 2)
  44. Transient transfection of linearized plasmids - (reply: 3)
  45. HUVEC plasmid transfection challenges - (reply: 2)
  46. Magnet assisted transfection - (reply: 1)
  47. Trouble with shRNA transfection - (reply: 2)
  48. Transient Transfection Controls (Gateway Technology) - (reply: 4)
  49. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  50. To small amount of cells used for shRNA transfection - (reply: 1)
  51. Dual Luciferase Assay - (reply: 1)
  52. Determining the amount of plasmid transfected - (reply: 1)
  53. Can I scale up at transfection, after determining the drug concentration via kil - (reply: 2)
  54. Luciferase assay - extreme variation in RLU - (reply: 1)
  55. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  56. Insect cells transfection reagent - (reply: 1)
  57. primary valve interstitial cells transfection - (reply: 1)
  58. Problem in transfection for producing lentivirus - (reply: 4)
  59. Transfection of a non human gene into human cell line - (reply: 1)
  60. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
  61. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  62. Recommendation on siRNA transfection reagents - (reply: 2)
  63. lipofectamine 2000:DNA complex stability - (reply: 2)
  64. Transfection reagent stored at -20C - (reply: 2)
  65. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  66. Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 - (reply: 1)
  67. transient transfection problem using ME180 cells - (reply: 1)
  68. Linearising plasmid before transfection? - (reply: 2)
  69. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  70. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 1)
  71. Fugene transfections - (reply: 1)
  72. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  73. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  74. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  75. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  76. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
  77. Dual Luciferase assay data analysis issues - (reply: 4)
  78. HEK 293T Transfection problem - (reply: 4)
  79. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  80. Luciferase: too much pbs - (reply: 4)
  81. Transfection Rate - (reply: 1)
  82. Calcium Phosphate transfection - storage? - (reply: 1)
  83. Calcium chloride plasmid transfection - (reply: 1)
  84. virus transduction vs plasmid transfection - (reply: 4)
  85. Luciferase Assay Weird Result: TK-Renilla Changing More than SV40 Firefly - (reply: 1)
  86. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  87. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  88. Stable transfection - (reply: 2)
  89. Luciferase assay-normalisation - (reply: 2)
  90. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  91. Transfection with T-PRO reagent - Any tips? - (reply: 1)
  92. Cotransient Transfections DNA Requirements - (reply: 3)
  93. Help with Dual luciferase assay - (reply: 1)
  94. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  95. Luciferase assay - expected range of raw reading values - (reply: 1)
  96. Calculating siRNA Transfection Efficiency - (reply: 3)
  97. LENTIVIRUS transfection efficiency - (reply: 1)
  98. Transfection problem after transformation! - (reply: 5)
  99. Transfection - (reply: 1)
  100. Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
  101. Measure transfection effiicency - referee's question - (reply: 1)
  102. cells die after transfection - (reply: 1)
  103. What is cotransfection in molecular cell biology? - (reply: 1)
  104. stable transfection - (reply: 1)
  105. Chloroquine use in transfection!!! - (reply: 2)
  106. Problems with double transfections -cells die - (reply: 7)
  107. NIH 3T3 Stable Line Transfection - (reply: 1)
  108. GFP and luciferase measurements - (reply: 3)
  109. extract plasmids from transfected eukaryotic cells - (reply: 6)
  110. identification of the transfection efficiency - (reply: 4)
  111. stable transfection suspension cultures - (reply: 1)
  112. problem with transient transfection - (reply: 6)
  113. Problem in Stable transfection - (reply: 3)
  114. Transfection & Cilia help! - (reply: 3)
  115. Storage of transfected PC12 cells - (reply: 1)
  116. Transfection - Optimising Transfection Protocol (reply: 2)
  117. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  118. problem with siRNA transfection of HEK cells - (reply: 5)
  119. Viral transfection to neurosphere in vitro - (reply: 1)
  120. What transcription factors are involved in transient transfection - (reply: 1)
  121. NMDA Transfection in 293 Cells - (reply: 1)
  122. Lipofectamine and transfection - (reply: 2)
  123. problems at normalizing luciferase assay data - (reply: 1)
  124. dual luciferase data analysis - (reply: 2)
  125. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
  126. Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
  127. Big plasmids and Transfection - (reply: 3)
  128. CaMKII alpha promoter and cell tranfection - (reply: 1)
  129. How to explain this luciferase result?! - (reply: 2)
  130. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  131. MDA-MB-231 cell transfection - (reply: 4)
  132. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  133. why these guys use T7 instead of CMV? - for transfection (reply: 2)
  134. Luciferase assay - (reply: 2)
  135. NIH3T3 Transfection - (reply: 1)
  136. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  137. siRNA transfection with lipofectamine 2000 - (reply: 1)
  138. Will transfected plasmids induce cytokine production? - (reply: 1)
  139. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  140. Transfection in insect cell lines?? - (reply: 1)
  141. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  142. ShRNA transfection - (reply: 1)
  143. Luciferase Assay Stim. Concentration? - (reply: 1)
  144. miRNA/siRNA transfection doubts - (reply: 2)
  145. Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
  146. TE buffer in PEI transfection - (reply: 1)
  147. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
  148. Virus with serum - viral transfection (reply: 1)
  149. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  150. Neuron transfection method-need the best protocol - (reply: 4)
  151. 3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
  152. transfection or infection - difference? (reply: 1)
  153. DharmaFECT transfection reagent - (reply: 1)
  154. stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
  155. luciferase GPCR assays - (reply: 1)
  156. Problems with transfection in human cells - (reply: 3)
  157. Preferences for MEF transfections - (reply: 1)
  158. CaPO4 transfection - (reply: 1)
  159. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  160. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  161. 293 Transfection Troubleshoot ! - (reply: 2)
  162. Luciferase and cell responsiveness - (reply: 3)
  163. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  164. Transfection Problem - (reply: 3)
  165. 293T transfection - (reply: 1)
  166. lenti virus transfected cell dies... - (reply: 4)
  167. how to compensate the cell death during transfection by electroporation - (reply: 2)
  168. how to minimize the variation among the replicas - transcient transfection (reply: 4)
  169. Transfection - (reply: 1)
  170. normalization of dual luciferase assay - (reply: 4)
  171. dual luciferase reporter?? - (reply: 2)
  172. Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
  173. Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
  174. Methylation and luciferase reporter query - (reply: 4)
  175. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  176. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  177. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  178. explain Transient transfection - (reply: 1)
  179. Help! low efficiency of transfection - (reply: 2)
  180. pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
  181. Stable transfection selection - (reply: 3)
  182. transfection is not working - (reply: 4)
  183. Transfection didnt work after repeating it. - (reply: 11)
  184. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  185. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  186. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  187. suspension cell transient transfection - (reply: 10)
  188. After Transfection - (reply: 1)
  189. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  190. Early transfection - (reply: 6)
  191. Plant cell transfection - (reply: 1)
  192. luciferase - (reply: 1)
  193. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  194. Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
  195. Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
  196. Basic questions about Transfection - About Lipofectamine (reply: 3)
  197. GFP and Luciferase Cell Line - (reply: 2)
  198. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  199. Luciferase signal - (reply: 5)
  200. Transfection into eukaryotic cells - (reply: 1)
  201. Lentivirus Production: Problems / Transfection Method - (reply: 4)
  202. Normal 96-well compatible for luciferase assay? - (reply: 3)
  203. Transfection - (reply: 1)
  204. Double transfection of siRNA - (reply: 2)
  205. NIH3t3 transfection optimization - (reply: 1)
  206. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  207. problem in plasmid transfection - (reply: 2)
  208. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  209. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  210. How to normalize my samples before I transfect my cells - (reply: 6)
  211. transfection efficiency - (reply: 1)
  212. transfection time - (reply: 2)
  213. problem in transfection - (reply: 4)
  214. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  215. transient transfection - (reply: 1)
  216. stable transfection problem - (reply: 1)
  217. Luciferase transfection controls - (reply: 3)
  218. checking transient transfection efficiency - (reply: 3)
  219. Immunofluorescence with transfected cells - (reply: 3)
  220. GFP constructs in Luciferase assay - (reply: 4)
  221. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  222. suspension cell transient transfection - (reply: 2)
  223. cell cryopreservation after siRNA transfection - (reply: 1)
  224. Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
  225. lipofectamine and PEI transfection of hela cells - (reply: 2)
  226. Hoe much media needed for transfection complex? - transfection (reply: 9)
  227. Neon Transfection System - (reply: 2)
  228. Transfection before plating? - (reply: 2)
  229. Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
  230. Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
  231. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  232. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  233. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  234. Dual Luciferase Assay - (reply: 3)
  235. Transfection - Plasmid Uptake Control - (reply: 2)
  236. Luciferase GL4 siRNA - (reply: 5)
  237. Two virus method for transfection - (reply: 1)
  238. luciferase assay - basic information on luciferase assay (reply: 3)
  239. plasmid purification kit for transfection - (reply: 2)
  240. best plasmid purification kit for transfection - (reply: 1)
  241. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  242. Transfection: How long until translation starts? - (reply: 2)
  243. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
  244. Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
  245. How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
  246. Tranfection cell density and overgrowth - (reply: 3)
  247. Double transfection questions - (reply: 2)
  248. U2OS transient transfection - (reply: 6)
  249. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  250. How good are CHO cells - in general and for transfection (reply: 1)
  251. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
  252. Promoter Cloning for Luciferase Assay - (reply: 3)
  253. siRNA and plasmid cotransfection - (reply: 1)
  254. Tranfection of HL 60 cells - (reply: 1)
  255. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
  256. Transfection of NIH/3T3 - (reply: 2)
  257. Transfection problems - (reply: 5)
  258. expression of SV40 in luciferase assay - (reply: 3)
  259. Minimum transfection incubation time - (reply: 2)
  260. Transfection - (reply: 7)
  261. about measuring luciferase activity in living cells - (reply: 8)
  262. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  263. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  264. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  265. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
  266. Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
  267. transient transfection - (reply: 2)
  268. Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
  269. HepG2 siRNA transfection - (reply: 1)
  270. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  271. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
  272. Max. length for 3'UTR in luciferase assay? - (reply: 1)
  273. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  274. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  275. Luciferase assay - (reply: 3)
  276. Transfection problem - milky media - (reply: 1)
  277. stable transfection problems - stable knockdown (reply: 3)
  278. Luciferase reporter induced by betagal vector?? - (reply: 1)
  279. HeLa tranfection- Dual luciferase Assay - (reply: 2)
  280. transfection in floating cells - (reply: 4)
  281. Transfection Expression Level Issues - (reply: 6)
  282. Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
  283. complicated transfection requires professional help - (reply: 3)
  284. transient transfection efficiency - (reply: 2)
  285. Filtration DNA before transfection? - (reply: 4)
  286. 293s, transfection and MTT assay - help! (reply: 3)
  287. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  288. pIRESneo for mammalian cells transfection - (reply: 4)
  289. How to transfect two plasmids in a cell - (reply: 10)
  290. SKBR3 transfection - (reply: 1)
  291. lipofectamine - (reply: 3)
  292. fibroblasts transfection - (reply: 1)
  293. Firefly Luciferase Assay - variation in FL reading (reply: 2)
  294. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  295. transfection - (reply: 1)
  296. transfection - (reply: 1)
  297. Heterogenous expresion of stably transfected gene - (reply: 4)
  298. Retroviral vector with luciferase - (reply: 1)
  299. Transfection efficiency determination - (reply: 4)
  300. Transfection contamination? - (reply: 12)
  301. Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
  302. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  303. stable transfectants - (reply: 6)
  304. Quick lipofectamine question - (reply: 7)
  305. Transfection AML-193 - (reply: 3)
  306. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
  307. Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
  308. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  309. Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
  310. Target gene regulation by transiently transfected PR - (reply: 4)
  311. Transfection of vectors - (reply: 8)
  312. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  313. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  314. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  315. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  316. Hygromycin selection - Selection 72 hours after transfection (reply: 3)
  317. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  318. stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
  319. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)