Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Transfection Related Discussions
  1. Plasmid distributors for Luciferase Assay? - (reply: 2)
  2. Transfection Vs Transduction - (reply: 1)
  3. Neuron transfection - (reply: 1)
  4. Viscous plasmids and transfection issues - (reply: 2)
  5. Transfection - (reply: 2)
  6. Transfection help - (reply: 1)
  7. Purification of linearized AT-rich DNA for transfection - (reply: 4)
  8. transfection - (reply: 2)
  9. Lipofectamine 3000 Protocol Written Out With Timing Specifics Regarding the Tran - (reply: 1)
  10. Luciferase assay - experimental design - (reply: 1)
  11. Transfection with Lipofectamine and PEI, differences - (reply: 2)
  12. Luciferase assay cloning - (reply: 2)
  13. What make a plasmid candidate for stable transfection - (reply: 1)
  14. Transfection with lipofectamine - (reply: 1)
  15. Why are my Huh 7s dying after transfections? - (reply: 1)
  16. Storing Ligated/Transformation Material before Transfection - (reply: 1)
  17. transfection of suspended CHO cells - (reply: 2)
  18. Hek293 transfection help!!!! - (reply: 1)
  19. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  20. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  21. Help with HUVEC cell transfection - (reply: 3)
  22. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  23. GFP interference in dual luciferase assay? - (reply: 2)
  24. preparing a vector for transfection - (reply: 2)
  25. Easy to transfect primary cell line? - (reply: 1)
  26. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  27. transfection of cells with two fluorescent reporters - (reply: 1)
  28. Stably transfected cells start dying - (reply: 6)
  29. not successful with transfection - (reply: 5)
  30. Transient transfection in huvec - (reply: 1)
  31. Blasticidin dose for Transfected - (reply: 1)
  32. Transfection problem - (reply: 6)
  33. Transfection media change - (reply: 1)
  34. Transfection of SH-SY5Y cells - (reply: 4)
  35. help for chip assay and luciferase assy - (reply: 1)
  36. Transfection help - (reply: 4)
  37. Viral RNA in total RNA eluate for transfection - (reply: 1)
  38. Stability of transient shRNA transfection - (reply: 1)
  39. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  40. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  41. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  42. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  43. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  44. Transfection Enhancer Efficiency - (reply: 1)
  45. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
  46. CHO-K1 transfection problem - (reply: 2)
  47. Using pBABE plasmids for regular transfection - (reply: 1)
  48. Subsequent DNA and siRNA transfection - (reply: 9)
  49. need help for transfection to synchronized cells - (reply: 4)
  50. 293t transfected with prtTA inducible vex - (reply: 5)
  51. No expression in stable transfected cell line - (reply: 2)
  52. Dead cells after transfection - (reply: 9)
  53. preparation of cells for FACS analysis after transfection - (reply: 4)
  54. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
  55. Transient transfection with large plasmid - (reply: 3)
  56. transfection using lipofectamine 2000 - (reply: 3)
  57. Determining phosphorylation status of a protein by transfection - (reply: 5)
  58. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  59. PGL-3 Luciferase Inactivity - (reply: 2)
  60. What is the optimal time for transfection efficiency measuring? - (reply: 1)
  61. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
  62. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  63. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  64. Transfection but no expression - (reply: 4)
  65. Cells Detach After Transfection - (reply: 1)
  66. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  67. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  68. Transfection methods for human ESCS of iPSCs - (reply: 1)
  69. Simultaneous RNA and DNA transfection? - (reply: 2)
  70. death of transfected cells after first passage in G418 medium - (reply: 1)
  71. transfection with lipofectamine 2000 advice - (reply: 4)
  72. clonogenic assay for shRNA transfected cells - (reply: 3)
  73. Luciferase fusion protein? - (reply: 1)
  74. How did they do this? Microinjection? Transfection? - (reply: 2)
  75. Luciferase assay problem - (reply: 2)
  76. Transient transfection of linearized plasmids - (reply: 3)
  77. HUVEC plasmid transfection challenges - (reply: 2)
  78. Magnet assisted transfection - (reply: 1)
  79. Trouble with shRNA transfection - (reply: 2)
  80. Transient Transfection Controls (Gateway Technology) - (reply: 4)
  81. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  82. To small amount of cells used for shRNA transfection - (reply: 1)
  83. Dual Luciferase Assay - (reply: 1)
  84. Determining the amount of plasmid transfected - (reply: 1)
  85. Can I scale up at transfection, after determining the drug concentration via kil - (reply: 2)
  86. Luciferase assay - extreme variation in RLU - (reply: 1)
  87. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  88. Insect cells transfection reagent - (reply: 1)
  89. primary valve interstitial cells transfection - (reply: 1)
  90. Problem in transfection for producing lentivirus - (reply: 4)
  91. Transfection of a non human gene into human cell line - (reply: 1)
  92. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
  93. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  94. Recommendation on siRNA transfection reagents - (reply: 2)
  95. lipofectamine 2000:DNA complex stability - (reply: 2)
  96. Transfection reagent stored at -20C - (reply: 2)
  97. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  98. Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 - (reply: 1)
  99. transient transfection problem using ME180 cells - (reply: 1)
  100. Linearising plasmid before transfection? - (reply: 2)
  101. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  102. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  103. Fugene transfections - (reply: 1)
  104. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  105. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  106. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  107. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  108. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
  109. Dual Luciferase assay data analysis issues - (reply: 4)
  110. HEK 293T Transfection problem - (reply: 4)
  111. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  112. Luciferase: too much pbs - (reply: 4)
  113. Transfection Rate - (reply: 1)
  114. Calcium Phosphate transfection - storage? - (reply: 1)
  115. Calcium chloride plasmid transfection - (reply: 1)
  116. virus transduction vs plasmid transfection - (reply: 4)
  117. Luciferase Assay Weird Result: TK-Renilla Changing More than SV40 Firefly - (reply: 1)
  118. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  119. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  120. Stable transfection - (reply: 2)
  121. Luciferase assay-normalisation - (reply: 2)
  122. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  123. Transfection with T-PRO reagent - Any tips? - (reply: 1)
  124. Cotransient Transfections DNA Requirements - (reply: 3)
  125. Help with Dual luciferase assay - (reply: 1)
  126. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  127. Luciferase assay - expected range of raw reading values - (reply: 1)
  128. Calculating siRNA Transfection Efficiency - (reply: 3)
  129. LENTIVIRUS transfection efficiency - (reply: 1)
  130. Transfection problem after transformation! - (reply: 5)
  131. Transfection - (reply: 1)
  132. Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
  133. Measure transfection effiicency - referee's question - (reply: 1)
  134. cells die after transfection - (reply: 1)
  135. What is cotransfection in molecular cell biology? - (reply: 1)
  136. stable transfection - (reply: 1)
  137. Chloroquine use in transfection!!! - (reply: 2)
  138. Problems with double transfections -cells die - (reply: 7)
  139. NIH 3T3 Stable Line Transfection - (reply: 1)
  140. GFP and luciferase measurements - (reply: 3)
  141. extract plasmids from transfected eukaryotic cells - (reply: 6)
  142. identification of the transfection efficiency - (reply: 4)
  143. stable transfection suspension cultures - (reply: 1)
  144. problem with transient transfection - (reply: 6)
  145. Problem in Stable transfection - (reply: 3)
  146. Transfection & Cilia help! - (reply: 3)
  147. Storage of transfected PC12 cells - (reply: 1)
  148. Transfection - Optimising Transfection Protocol (reply: 2)
  149. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  150. problem with siRNA transfection of HEK cells - (reply: 5)
  151. Viral transfection to neurosphere in vitro - (reply: 1)
  152. What transcription factors are involved in transient transfection - (reply: 1)
  153. NMDA Transfection in 293 Cells - (reply: 1)
  154. Lipofectamine and transfection - (reply: 2)
  155. problems at normalizing luciferase assay data - (reply: 1)
  156. dual luciferase data analysis - (reply: 2)
  157. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
  158. Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
  159. Big plasmids and Transfection - (reply: 3)
  160. CaMKII alpha promoter and cell tranfection - (reply: 1)
  161. How to explain this luciferase result?! - (reply: 2)
  162. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  163. MDA-MB-231 cell transfection - (reply: 5)
  164. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  165. why these guys use T7 instead of CMV? - for transfection (reply: 2)
  166. Luciferase assay - (reply: 2)
  167. NIH3T3 Transfection - (reply: 1)
  168. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  169. siRNA transfection with lipofectamine 2000 - (reply: 1)
  170. Will transfected plasmids induce cytokine production? - (reply: 1)
  171. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  172. Transfection in insect cell lines?? - (reply: 1)
  173. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  174. ShRNA transfection - (reply: 1)
  175. Luciferase Assay Stim. Concentration? - (reply: 1)
  176. miRNA/siRNA transfection doubts - (reply: 2)
  177. Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
  178. TE buffer in PEI transfection - (reply: 1)
  179. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
  180. Virus with serum - viral transfection (reply: 1)
  181. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  182. Neuron transfection method-need the best protocol - (reply: 4)
  183. 3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
  184. transfection or infection - difference? (reply: 1)
  185. DharmaFECT transfection reagent - (reply: 1)
  186. stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
  187. luciferase GPCR assays - (reply: 1)
  188. Problems with transfection in human cells - (reply: 3)
  189. Preferences for MEF transfections - (reply: 1)
  190. CaPO4 transfection - (reply: 1)
  191. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  192. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  193. 293 Transfection Troubleshoot ! - (reply: 2)
  194. Luciferase and cell responsiveness - (reply: 3)
  195. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  196. Transfection Problem - (reply: 3)
  197. 293T transfection - (reply: 1)
  198. lenti virus transfected cell dies... - (reply: 4)
  199. how to compensate the cell death during transfection by electroporation - (reply: 2)
  200. how to minimize the variation among the replicas - transcient transfection (reply: 4)
  201. Transfection - (reply: 1)
  202. normalization of dual luciferase assay - (reply: 4)
  203. dual luciferase reporter?? - (reply: 2)
  204. Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
  205. Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
  206. Methylation and luciferase reporter query - (reply: 4)
  207. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  208. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  209. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  210. explain Transient transfection - (reply: 1)
  211. Help! low efficiency of transfection - (reply: 2)
  212. pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
  213. Stable transfection selection - (reply: 3)
  214. transfection is not working - (reply: 4)
  215. Transfection didnt work after repeating it. - (reply: 11)
  216. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  217. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  218. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  219. suspension cell transient transfection - (reply: 10)
  220. After Transfection - (reply: 1)
  221. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  222. Early transfection - (reply: 6)
  223. Plant cell transfection - (reply: 1)
  224. luciferase - (reply: 1)
  225. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  226. Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
  227. Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
  228. Basic questions about Transfection - About Lipofectamine (reply: 3)
  229. GFP and Luciferase Cell Line - (reply: 2)
  230. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  231. Luciferase signal - (reply: 5)
  232. Transfection into eukaryotic cells - (reply: 1)
  233. Lentivirus Production: Problems / Transfection Method - (reply: 4)
  234. Normal 96-well compatible for luciferase assay? - (reply: 3)
  235. Transfection - (reply: 1)
  236. Double transfection of siRNA - (reply: 2)
  237. NIH3t3 transfection optimization - (reply: 1)
  238. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  239. problem in plasmid transfection - (reply: 2)
  240. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  241. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  242. How to normalize my samples before I transfect my cells - (reply: 6)
  243. transfection efficiency - (reply: 1)
  244. transfection time - (reply: 2)
  245. problem in transfection - (reply: 4)
  246. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  247. transient transfection - (reply: 1)
  248. stable transfection problem - (reply: 1)
  249. Luciferase transfection controls - (reply: 3)
  250. checking transient transfection efficiency - (reply: 3)
  251. Immunofluorescence with transfected cells - (reply: 3)
  252. GFP constructs in Luciferase assay - (reply: 4)
  253. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  254. suspension cell transient transfection - (reply: 2)
  255. cell cryopreservation after siRNA transfection - (reply: 1)
  256. Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
  257. lipofectamine and PEI transfection of hela cells - (reply: 2)
  258. Hoe much media needed for transfection complex? - transfection (reply: 9)
  259. Neon Transfection System - (reply: 2)
  260. Transfection before plating? - (reply: 2)
  261. Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
  262. Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
  263. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  264. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  265. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  266. Dual Luciferase Assay - (reply: 3)
  267. Transfection - Plasmid Uptake Control - (reply: 2)
  268. Luciferase GL4 siRNA - (reply: 5)
  269. Two virus method for transfection - (reply: 1)
  270. luciferase assay - basic information on luciferase assay (reply: 3)
  271. plasmid purification kit for transfection - (reply: 2)
  272. best plasmid purification kit for transfection - (reply: 1)
  273. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  274. Transfection: How long until translation starts? - (reply: 2)
  275. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
  276. Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
  277. How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
  278. Tranfection cell density and overgrowth - (reply: 3)
  279. Double transfection questions - (reply: 2)
  280. U2OS transient transfection - (reply: 6)
  281. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  282. How good are CHO cells - in general and for transfection (reply: 1)
  283. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
  284. Promoter Cloning for Luciferase Assay - (reply: 3)
  285. siRNA and plasmid cotransfection - (reply: 1)
  286. Tranfection of HL 60 cells - (reply: 1)
  287. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
  288. Transfection of NIH/3T3 - (reply: 2)
  289. Transfection problems - (reply: 5)
  290. expression of SV40 in luciferase assay - (reply: 3)
  291. Minimum transfection incubation time - (reply: 2)
  292. Transfection - (reply: 7)
  293. about measuring luciferase activity in living cells - (reply: 9)
  294. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  295. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  296. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  297. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
  298. Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
  299. transient transfection - (reply: 2)
  300. Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
  301. HepG2 siRNA transfection - (reply: 1)
  302. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  303. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
  304. Max. length for 3'UTR in luciferase assay? - (reply: 1)
  305. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  306. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  307. Luciferase assay - (reply: 3)
  308. Transfection problem - milky media - (reply: 1)
  309. stable transfection problems - stable knockdown (reply: 3)
  310. Luciferase reporter induced by betagal vector?? - (reply: 1)
  311. HeLa tranfection- Dual luciferase Assay - (reply: 2)
  312. transfection in floating cells - (reply: 5)
  313. Transfection Expression Level Issues - (reply: 6)
  314. Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
  315. complicated transfection requires professional help - (reply: 3)
  316. transient transfection efficiency - (reply: 2)
  317. Filtration DNA before transfection? - (reply: 4)
  318. 293s, transfection and MTT assay - help! (reply: 3)
  319. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  320. pIRESneo for mammalian cells transfection - (reply: 4)
  321. How to transfect two plasmids in a cell - (reply: 9)
  322. SKBR3 transfection - (reply: 1)
  323. lipofectamine - (reply: 3)
  324. fibroblasts transfection - (reply: 1)
  325. Firefly Luciferase Assay - variation in FL reading (reply: 2)
  326. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  327. transfection - (reply: 1)
  328. transfection - (reply: 1)
  329. Heterogenous expresion of stably transfected gene - (reply: 4)
  330. Retroviral vector with luciferase - (reply: 1)
  331. Transfection efficiency determination - (reply: 4)
  332. Transfection contamination? - (reply: 12)
  333. Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
  334. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  335. stable transfectants - (reply: 6)
  336. Quick lipofectamine question - (reply: 7)
  337. Transfection AML-193 - (reply: 3)
  338. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
  339. Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
  340. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  341. Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
  342. Target gene regulation by transiently transfected PR - (reply: 4)
  343. Transfection of vectors - (reply: 8)
  344. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  345. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  346. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  347. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  348. Hygromycin selection - Selection 72 hours after transfection (reply: 3)
  349. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  350. stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
  351. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)