|
Transfection Related Discussions
-
Luciferase assay-normalisation - (reply: 2)
-
Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
-
Transfection with T-PRO reagent - Any tips? - (reply: 1)
-
Cotransient Transfections DNA Requirements - (reply: 3)
-
Help with Dual luciferase assay - (reply: 1)
-
How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
-
Luciferase assay - expected range of raw reading values - (reply: 1)
-
Calculating siRNA Transfection Efficiency - (reply: 3)
-
LENTIVIRUS transfection efficiency - (reply: 1)
-
Transfection problem after transformation! - (reply: 5)
-
Transfection - (reply: 1)
-
Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
-
Measure transfection effiicency - referee's question - (reply: 1)
-
cells die after transfection - (reply: 1)
-
What is cotransfection in molecular cell biology? - (reply: 1)
-
stable transfection - (reply: 1)
-
Chloroquine use in transfection!!! - (reply: 2)
-
Problems with double transfections -cells die - (reply: 7)
-
NIH 3T3 Stable Line Transfection - (reply: 1)
-
GFP and luciferase measurements - (reply: 2)
-
extract plasmids from transfected eukaryotic cells - (reply: 6)
-
identification of the transfection efficiency - (reply: 4)
-
stable transfection suspension cultures - (reply: 1)
-
problem with transient transfection - (reply: 6)
-
Problem in Stable transfection - (reply: 2)
-
Transfection & Cilia help! - (reply: 3)
-
Storage of transfected PC12 cells - (reply: 1)
-
Transfection - Optimising Transfection Protocol (reply: 2)
-
Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
-
problem with siRNA transfection of HEK cells - (reply: 5)
-
Viral transfection to neurosphere in vitro - (reply: 1)
-
What transcription factors are involved in transient transfection - (reply: 1)
-
NMDA Transfection in 293 Cells - (reply: 1)
-
Lipofectamine and transfection - (reply: 2)
-
problems at normalizing luciferase assay data - (reply: 1)
-
dual luciferase data analysis - (reply: 2)
-
renilla values are going down with firefly values after miRNA transfection - (reply: 1)
-
Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
-
Big plasmids and Transfection - (reply: 3)
-
CaMKII alpha promoter and cell tranfection - (reply: 1)
-
How to explain this luciferase result?! - (reply: 2)
-
Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
-
MDA-MB-231 cell transfection - (reply: 4)
-
siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
-
why these guys use T7 instead of CMV? - for transfection (reply: 2)
-
Luciferase assay - (reply: 2)
-
NIH3T3 Transfection - (reply: 1)
-
Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
-
siRNA transfection with lipofectamine 2000 - (reply: 1)
-
Will transfected plasmids induce cytokine production? - (reply: 1)
-
Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
-
Transfection in insect cell lines?? - (reply: 1)
-
is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
-
ShRNA transfection - (reply: 1)
-
Luciferase Assay Stim. Concentration? - (reply: 1)
-
miRNA/siRNA transfection doubts - (reply: 2)
-
Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
-
TE buffer in PEI transfection - (reply: 1)
-
problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
-
Virus with serum - viral transfection (reply: 1)
-
Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
-
Neuron transfection method-need the best protocol - (reply: 4)
-
3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
-
transfection or infection - difference? (reply: 1)
-
DharmaFECT transfection reagent - (reply: 1)
-
stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
-
luciferase GPCR assays - (reply: 1)
-
Problems with transfection in human cells - (reply: 3)
-
Preferences for MEF transfections - (reply: 1)
-
CaPO4 transfection - (reply: 1)
-
Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
-
microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
-
293 Transfection Troubleshoot ! - (reply: 2)
-
Luciferase and cell responsiveness - (reply: 3)
-
Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
-
Transfection Problem - (reply: 3)
-
293T transfection - (reply: 1)
-
lenti virus transfected cell dies... - (reply: 4)
-
how to compensate the cell death during transfection by electroporation - (reply: 2)
-
how to minimize the variation among the replicas - transcient transfection (reply: 4)
-
Transfection - (reply: 1)
-
normalization of dual luciferase assay - (reply: 4)
-
dual luciferase reporter?? - (reply: 2)
-
Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
-
Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
-
Methylation and luciferase reporter query - (reply: 4)
-
why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
-
tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
-
transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
-
explain Transient transfection - (reply: 1)
-
Help! low efficiency of transfection - (reply: 2)
-
pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
-
Stable transfection selection - (reply: 3)
-
transfection is not working - (reply: 4)
-
Transfection didnt work after repeating it. - (reply: 11)
-
Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
-
Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
-
Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
-
suspension cell transient transfection - (reply: 9)
-
After Transfection - (reply: 1)
-
Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
-
Early transfection - (reply: 6)
-
Plant cell transfection - (reply: 1)
-
luciferase - (reply: 1)
-
cloned plasmid expression less than original plasmid - after transfection (reply: 4)
-
Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
-
Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
-
Basic questions about Transfection - About Lipofectamine (reply: 3)
-
GFP and Luciferase Cell Line - (reply: 2)
-
Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
-
Luciferase signal - (reply: 5)
-
Transfection into eukaryotic cells - (reply: 1)
-
Lentivirus Production: Problems / Transfection Method - (reply: 4)
-
Normal 96-well compatible for luciferase assay? - (reply: 3)
-
Transfection - (reply: 1)
-
Double transfection of siRNA - (reply: 2)
-
NIH3t3 transfection optimization - (reply: 1)
-
Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
-
problem in plasmid transfection - (reply: 2)
-
Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
-
Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
-
How to normalize my samples before I transfect my cells - (reply: 6)
-
transfection efficiency - (reply: 1)
-
transfection time - (reply: 2)
-
problem in transfection - (reply: 4)
-
No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
-
transient transfection - (reply: 1)
-
stable transfection problem - (reply: 1)
-
Luciferase transfection controls - (reply: 2)
-
checking transient transfection efficiency - (reply: 3)
-
Immunofluorescence with transfected cells - (reply: 3)
-
GFP constructs in Luciferase assay - (reply: 4)
-
Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
-
suspension cell transient transfection - (reply: 2)
-
cell cryopreservation after siRNA transfection - (reply: 1)
-
Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
-
lipofectamine and PEI transfection of hela cells - (reply: 2)
-
Hoe much media needed for transfection complex? - transfection (reply: 9)
-
Neon Transfection System - (reply: 2)
-
Transfection before plating? - (reply: 2)
-
Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
-
Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
-
Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
-
Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
-
nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
-
Dual Luciferase Assay - (reply: 3)
-
Transfection - Plasmid Uptake Control - (reply: 2)
-
Luciferase GL4 siRNA - (reply: 5)
-
Two virus method for transfection - (reply: 1)
-
luciferase assay - basic information on luciferase assay (reply: 3)
-
plasmid purification kit for transfection - (reply: 2)
-
best plasmid purification kit for transfection - (reply: 1)
-
Difficulties with the transfection of cells CHO DG44 - (reply: 9)
-
Transfection: How long until translation starts? - (reply: 2)
-
Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
-
Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
-
How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
-
Tranfection cell density and overgrowth - (reply: 3)
-
Double transfection questions - (reply: 2)
-
U2OS transient transfection - (reply: 6)
-
Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
-
How good are CHO cells - in general and for transfection (reply: 1)
-
Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
-
Promoter Cloning for Luciferase Assay - (reply: 3)
-
siRNA and plasmid cotransfection - (reply: 1)
-
Tranfection of HL 60 cells - (reply: 1)
-
Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
-
Transfection of NIH/3T3 - (reply: 2)
-
Transfection problems - (reply: 5)
-
expression of SV40 in luciferase assay - (reply: 3)
-
Minimum transfection incubation time - (reply: 2)
-
Transfection - (reply: 7)
-
about measuring luciferase activity in living cells - (reply: 8)
-
MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
-
Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
-
Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
-
Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
-
Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
-
transient transfection - (reply: 2)
-
Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
-
HepG2 siRNA transfection - (reply: 1)
-
Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
-
Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
-
Max. length for 3'UTR in luciferase assay? - (reply: 1)
-
Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
-
Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
-
Luciferase assay - (reply: 3)
-
Transfection problem - milky media - (reply: 1)
-
stable transfection problems - stable knockdown (reply: 3)
-
Luciferase reporter induced by betagal vector?? - (reply: 1)
-
HeLa tranfection- Dual luciferase Assay - (reply: 2)
-
transfection in floating cells - (reply: 4)
-
Transfection Expression Level Issues - (reply: 6)
-
Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
-
complicated transfection requires professional help - (reply: 3)
-
transient transfection efficiency - (reply: 2)
-
Filtration DNA before transfection? - (reply: 4)
-
293s, transfection and MTT assay - help! (reply: 3)
-
Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
-
pIRESneo for mammalian cells transfection - (reply: 4)
-
How to transfect two plasmids in a cell - (reply: 10)
-
SKBR3 transfection - (reply: 1)
-
lipofectamine - (reply: 3)
-
fibroblasts transfection - (reply: 1)
-
Firefly Luciferase Assay - variation in FL reading (reply: 2)
-
cell line for luciferase assay - How to choose the best cell line? (reply: 2)
-
transfection - (reply: 1)
-
transfection - (reply: 1)
-
Heterogenous expresion of stably transfected gene - (reply: 4)
-
Retroviral vector with luciferase - (reply: 1)
-
Transfection efficiency determination - (reply: 4)
-
Transfection contamination? - (reply: 12)
-
Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
-
siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
-
stable transfectants - (reply: 6)
-
Quick lipofectamine question - (reply: 7)
-
Transfection AML-193 - (reply: 3)
-
stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
-
Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
-
Make construct so it is only thing that can PCR after transfection - (reply: 2)
-
Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
-
Target gene regulation by transiently transfected PR - (reply: 4)
-
Transfection of vectors - (reply: 8)
-
Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
-
Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
-
Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
-
SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
-
Hygromycin selection - Selection 72 hours after transfection (reply: 3)
-
293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
-
stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
-
In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)