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Transfection Related Discussions
  1. Luciferase assay - experimental design - (reply: 1)
  2. Transfection with Lipofectamine and PEI, differences - (reply: 2)
  3. Luciferase assay cloning - (reply: 2)
  4. What make a plasmid candidate for stable transfection - (reply: 1)
  5. Transfection with lipofectamine - (reply: 1)
  6. Why are my Huh 7s dying after transfections? - (reply: 1)
  7. Storing Ligated/Transformation Material before Transfection - (reply: 1)
  8. transfection of suspended CHO cells - (reply: 2)
  9. Hek293 transfection help!!!! - (reply: 1)
  10. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  11. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  12. Help with HUVEC cell transfection - (reply: 3)
  13. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  14. GFP interference in dual luciferase assay? - (reply: 2)
  15. preparing a vector for transfection - (reply: 2)
  16. Easy to transfect primary cell line? - (reply: 1)
  17. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  18. transfection of cells with two fluorescent reporters - (reply: 1)
  19. Stably transfected cells start dying - (reply: 6)
  20. not successful with transfection - (reply: 5)
  21. Transient transfection in huvec - (reply: 1)
  22. Blasticidin dose for Transfected - (reply: 1)
  23. Transfection problem - (reply: 6)
  24. Transfection media change - (reply: 1)
  25. Transfection of SH-SY5Y cells - (reply: 4)
  26. help for chip assay and luciferase assy - (reply: 1)
  27. Transfection help - (reply: 4)
  28. Viral RNA in total RNA eluate for transfection - (reply: 1)
  29. Stability of transient shRNA transfection - (reply: 1)
  30. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  31. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  32. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  33. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  34. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  35. Transfection Enhancer Efficiency - (reply: 1)
  36. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
  37. CHO-K1 transfection problem - (reply: 2)
  38. Using pBABE plasmids for regular transfection - (reply: 1)
  39. Subsequent DNA and siRNA transfection - (reply: 9)
  40. need help for transfection to synchronized cells - (reply: 4)
  41. 293t transfected with prtTA inducible vex - (reply: 5)
  42. No expression in stable transfected cell line - (reply: 2)
  43. Dead cells after transfection - (reply: 9)
  44. preparation of cells for FACS analysis after transfection - (reply: 4)
  45. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
  46. Transient transfection with large plasmid - (reply: 3)
  47. transfection using lipofectamine 2000 - (reply: 3)
  48. Determining phosphorylation status of a protein by transfection - (reply: 5)
  49. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  50. PGL-3 Luciferase Inactivity - (reply: 2)
  51. What is the optimal time for transfection efficiency measuring? - (reply: 1)
  52. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
  53. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  54. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  55. Transfection but no expression - (reply: 4)
  56. Cells Detach After Transfection - (reply: 1)
  57. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  58. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  59. Transfection methods for human ESCS of iPSCs - (reply: 1)
  60. Simultaneous RNA and DNA transfection? - (reply: 2)
  61. death of transfected cells after first passage in G418 medium - (reply: 1)
  62. transfection with lipofectamine 2000 advice - (reply: 4)
  63. clonogenic assay for shRNA transfected cells - (reply: 3)
  64. Luciferase fusion protein? - (reply: 1)
  65. How did they do this? Microinjection? Transfection? - (reply: 2)
  66. Luciferase assay problem - (reply: 2)
  67. Transient transfection of linearized plasmids - (reply: 3)
  68. HUVEC plasmid transfection challenges - (reply: 2)
  69. Magnet assisted transfection - (reply: 1)
  70. Trouble with shRNA transfection - (reply: 2)
  71. Transient Transfection Controls (Gateway Technology) - (reply: 4)
  72. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  73. To small amount of cells used for shRNA transfection - (reply: 1)
  74. Dual Luciferase Assay - (reply: 1)
  75. Determining the amount of plasmid transfected - (reply: 1)
  76. Can I scale up at transfection, after determining the drug concentration via kil - (reply: 2)
  77. Luciferase assay - extreme variation in RLU - (reply: 1)
  78. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  79. Insect cells transfection reagent - (reply: 1)
  80. primary valve interstitial cells transfection - (reply: 1)
  81. Problem in transfection for producing lentivirus - (reply: 4)
  82. Transfection of a non human gene into human cell line - (reply: 1)
  83. Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
  84. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  85. Recommendation on siRNA transfection reagents - (reply: 2)
  86. lipofectamine 2000:DNA complex stability - (reply: 2)
  87. Transfection reagent stored at -20C - (reply: 2)
  88. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  89. Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 - (reply: 1)
  90. transient transfection problem using ME180 cells - (reply: 1)
  91. Linearising plasmid before transfection? - (reply: 2)
  92. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  93. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  94. Fugene transfections - (reply: 1)
  95. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  96. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  97. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  98. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  99. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
  100. Dual Luciferase assay data analysis issues - (reply: 4)
  101. HEK 293T Transfection problem - (reply: 4)
  102. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  103. Luciferase: too much pbs - (reply: 4)
  104. Transfection Rate - (reply: 1)
  105. Calcium Phosphate transfection - storage? - (reply: 1)
  106. Calcium chloride plasmid transfection - (reply: 1)
  107. virus transduction vs plasmid transfection - (reply: 4)
  108. Luciferase Assay Weird Result: TK-Renilla Changing More than SV40 Firefly - (reply: 1)
  109. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  110. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  111. Stable transfection - (reply: 2)
  112. Luciferase assay-normalisation - (reply: 2)
  113. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  114. Transfection with T-PRO reagent - Any tips? - (reply: 1)
  115. Cotransient Transfections DNA Requirements - (reply: 3)
  116. Help with Dual luciferase assay - (reply: 1)
  117. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  118. Luciferase assay - expected range of raw reading values - (reply: 1)
  119. Calculating siRNA Transfection Efficiency - (reply: 3)
  120. LENTIVIRUS transfection efficiency - (reply: 1)
  121. Transfection problem after transformation! - (reply: 5)
  122. Transfection - (reply: 1)
  123. Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
  124. Measure transfection effiicency - referee's question - (reply: 1)
  125. cells die after transfection - (reply: 1)
  126. What is cotransfection in molecular cell biology? - (reply: 1)
  127. stable transfection - (reply: 1)
  128. Chloroquine use in transfection!!! - (reply: 2)
  129. Problems with double transfections -cells die - (reply: 7)
  130. NIH 3T3 Stable Line Transfection - (reply: 1)
  131. GFP and luciferase measurements - (reply: 3)
  132. extract plasmids from transfected eukaryotic cells - (reply: 6)
  133. identification of the transfection efficiency - (reply: 4)
  134. stable transfection suspension cultures - (reply: 1)
  135. problem with transient transfection - (reply: 6)
  136. Problem in Stable transfection - (reply: 3)
  137. Transfection & Cilia help! - (reply: 3)
  138. Storage of transfected PC12 cells - (reply: 1)
  139. Transfection - Optimising Transfection Protocol (reply: 2)
  140. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
  141. problem with siRNA transfection of HEK cells - (reply: 5)
  142. Viral transfection to neurosphere in vitro - (reply: 1)
  143. What transcription factors are involved in transient transfection - (reply: 1)
  144. NMDA Transfection in 293 Cells - (reply: 1)
  145. Lipofectamine and transfection - (reply: 2)
  146. problems at normalizing luciferase assay data - (reply: 1)
  147. dual luciferase data analysis - (reply: 2)
  148. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
  149. Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
  150. Big plasmids and Transfection - (reply: 3)
  151. CaMKII alpha promoter and cell tranfection - (reply: 1)
  152. How to explain this luciferase result?! - (reply: 2)
  153. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  154. MDA-MB-231 cell transfection - (reply: 5)
  155. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  156. why these guys use T7 instead of CMV? - for transfection (reply: 2)
  157. Luciferase assay - (reply: 2)
  158. NIH3T3 Transfection - (reply: 1)
  159. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  160. siRNA transfection with lipofectamine 2000 - (reply: 1)
  161. Will transfected plasmids induce cytokine production? - (reply: 1)
  162. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  163. Transfection in insect cell lines?? - (reply: 1)
  164. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  165. ShRNA transfection - (reply: 1)
  166. Luciferase Assay Stim. Concentration? - (reply: 1)
  167. miRNA/siRNA transfection doubts - (reply: 2)
  168. Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
  169. TE buffer in PEI transfection - (reply: 1)
  170. problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
  171. Virus with serum - viral transfection (reply: 1)
  172. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  173. Neuron transfection method-need the best protocol - (reply: 4)
  174. 3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
  175. transfection or infection - difference? (reply: 1)
  176. DharmaFECT transfection reagent - (reply: 1)
  177. stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
  178. luciferase GPCR assays - (reply: 1)
  179. Problems with transfection in human cells - (reply: 3)
  180. Preferences for MEF transfections - (reply: 1)
  181. CaPO4 transfection - (reply: 1)
  182. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  183. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  184. 293 Transfection Troubleshoot ! - (reply: 2)
  185. Luciferase and cell responsiveness - (reply: 3)
  186. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  187. Transfection Problem - (reply: 3)
  188. 293T transfection - (reply: 1)
  189. lenti virus transfected cell dies... - (reply: 4)
  190. how to compensate the cell death during transfection by electroporation - (reply: 2)
  191. how to minimize the variation among the replicas - transcient transfection (reply: 4)
  192. Transfection - (reply: 1)
  193. normalization of dual luciferase assay - (reply: 4)
  194. dual luciferase reporter?? - (reply: 2)
  195. Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
  196. Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
  197. Methylation and luciferase reporter query - (reply: 4)
  198. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  199. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  200. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  201. explain Transient transfection - (reply: 1)
  202. Help! low efficiency of transfection - (reply: 2)
  203. pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
  204. Stable transfection selection - (reply: 3)
  205. transfection is not working - (reply: 4)
  206. Transfection didnt work after repeating it. - (reply: 11)
  207. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  208. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  209. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  210. suspension cell transient transfection - (reply: 10)
  211. After Transfection - (reply: 1)
  212. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
  213. Early transfection - (reply: 6)
  214. Plant cell transfection - (reply: 1)
  215. luciferase - (reply: 1)
  216. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  217. Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
  218. Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
  219. Basic questions about Transfection - About Lipofectamine (reply: 3)
  220. GFP and Luciferase Cell Line - (reply: 2)
  221. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  222. Luciferase signal - (reply: 5)
  223. Transfection into eukaryotic cells - (reply: 1)
  224. Lentivirus Production: Problems / Transfection Method - (reply: 4)
  225. Normal 96-well compatible for luciferase assay? - (reply: 3)
  226. Transfection - (reply: 1)
  227. Double transfection of siRNA - (reply: 2)
  228. NIH3t3 transfection optimization - (reply: 1)
  229. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  230. problem in plasmid transfection - (reply: 2)
  231. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  232. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  233. How to normalize my samples before I transfect my cells - (reply: 6)
  234. transfection efficiency - (reply: 1)
  235. transfection time - (reply: 2)
  236. problem in transfection - (reply: 4)
  237. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  238. transient transfection - (reply: 1)
  239. stable transfection problem - (reply: 1)
  240. Luciferase transfection controls - (reply: 3)
  241. checking transient transfection efficiency - (reply: 3)
  242. Immunofluorescence with transfected cells - (reply: 3)
  243. GFP constructs in Luciferase assay - (reply: 4)
  244. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  245. suspension cell transient transfection - (reply: 2)
  246. cell cryopreservation after siRNA transfection - (reply: 1)
  247. Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
  248. lipofectamine and PEI transfection of hela cells - (reply: 2)
  249. Hoe much media needed for transfection complex? - transfection (reply: 9)
  250. Neon Transfection System - (reply: 2)
  251. Transfection before plating? - (reply: 2)
  252. Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
  253. Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
  254. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  255. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  256. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  257. Dual Luciferase Assay - (reply: 3)
  258. Transfection - Plasmid Uptake Control - (reply: 2)
  259. Luciferase GL4 siRNA - (reply: 5)
  260. Two virus method for transfection - (reply: 1)
  261. luciferase assay - basic information on luciferase assay (reply: 3)
  262. plasmid purification kit for transfection - (reply: 2)
  263. best plasmid purification kit for transfection - (reply: 1)
  264. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  265. Transfection: How long until translation starts? - (reply: 2)
  266. Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
  267. Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
  268. How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
  269. Tranfection cell density and overgrowth - (reply: 3)
  270. Double transfection questions - (reply: 2)
  271. U2OS transient transfection - (reply: 6)
  272. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  273. How good are CHO cells - in general and for transfection (reply: 1)
  274. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
  275. Promoter Cloning for Luciferase Assay - (reply: 3)
  276. siRNA and plasmid cotransfection - (reply: 1)
  277. Tranfection of HL 60 cells - (reply: 1)
  278. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
  279. Transfection of NIH/3T3 - (reply: 2)
  280. Transfection problems - (reply: 5)
  281. expression of SV40 in luciferase assay - (reply: 3)
  282. Minimum transfection incubation time - (reply: 2)
  283. Transfection - (reply: 7)
  284. about measuring luciferase activity in living cells - (reply: 8)
  285. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  286. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  287. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  288. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
  289. Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
  290. transient transfection - (reply: 2)
  291. Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
  292. HepG2 siRNA transfection - (reply: 1)
  293. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  294. Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
  295. Max. length for 3'UTR in luciferase assay? - (reply: 1)
  296. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  297. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  298. Luciferase assay - (reply: 3)
  299. Transfection problem - milky media - (reply: 1)
  300. stable transfection problems - stable knockdown (reply: 3)
  301. Luciferase reporter induced by betagal vector?? - (reply: 1)
  302. HeLa tranfection- Dual luciferase Assay - (reply: 2)
  303. transfection in floating cells - (reply: 5)
  304. Transfection Expression Level Issues - (reply: 6)
  305. Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
  306. complicated transfection requires professional help - (reply: 3)
  307. transient transfection efficiency - (reply: 2)
  308. Filtration DNA before transfection? - (reply: 4)
  309. 293s, transfection and MTT assay - help! (reply: 3)
  310. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  311. pIRESneo for mammalian cells transfection - (reply: 4)
  312. How to transfect two plasmids in a cell - (reply: 10)
  313. SKBR3 transfection - (reply: 1)
  314. lipofectamine - (reply: 3)
  315. fibroblasts transfection - (reply: 1)
  316. Firefly Luciferase Assay - variation in FL reading (reply: 2)
  317. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  318. transfection - (reply: 1)
  319. transfection - (reply: 1)
  320. Heterogenous expresion of stably transfected gene - (reply: 4)
  321. Retroviral vector with luciferase - (reply: 1)
  322. Transfection efficiency determination - (reply: 4)
  323. Transfection contamination? - (reply: 12)
  324. Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
  325. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  326. stable transfectants - (reply: 6)
  327. Quick lipofectamine question - (reply: 7)
  328. Transfection AML-193 - (reply: 3)
  329. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
  330. Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
  331. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  332. Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
  333. Target gene regulation by transiently transfected PR - (reply: 4)
  334. Transfection of vectors - (reply: 8)
  335. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  336. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  337. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  338. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  339. Hygromycin selection - Selection 72 hours after transfection (reply: 3)
  340. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  341. stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
  342. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)