Transfection - (Jul/24/2017 )

Hi guys,

Can anyone let me know why transfection decrease after increasing time of incubation with formulation? One reason can be toxicity and is there any other reason too?

Depends on the method used - For lipofection (e.g. Lipofectamine) the lipospheres are unstable and break down over time. Electroporation reagents tend to be very toxic and the method creates big holes in the membranes. 

DNA also gets broken down by external DNases.

I'm using liposomes and doing betagal transfection