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Half-life of Transfected pre-miRNA and miRNA inhibitors - (Jan/22/2010 )

Hi,

Would anybody have any comments on the half-life of transfected (synthetic) pre-miRNAs and miRNA inhibitors in cells? This information is quite hard to find.
The cells I personally use are NT2/D1 and 2102Ep cells. I use the Pre-miRNAs from Ambion and miRIDIAN inhibitors from Dharmacon.

Thanks

-Baars01-

Baars01 on Jan 22 2010, 11:13 AM said:

Hi,

Would anybody have any comments on the half-life of transfected (synthetic) pre-miRNAs and miRNA inhibitors in cells? This information is quite hard to find.
The cells I personally use are NT2/D1 and 2102Ep cells. I use the Pre-miRNAs from Ambion and miRIDIAN inhibitors from Dharmacon.

Thanks


Really good question...to which I'd also like to know the answer!

-miRNA man-

me too.

perhaps you can tell us from your study.

thanks

-stylothecancer-