Half-life of Transfected pre-miRNA and miRNA inhibitors - (Jan/22/2010 )
Hi,
Would anybody have any comments on the half-life of transfected (synthetic) pre-miRNAs and miRNA inhibitors in cells? This information is quite hard to find.
The cells I personally use are NT2/D1 and 2102Ep cells. I use the Pre-miRNAs from Ambion and miRIDIAN inhibitors from Dharmacon.
Thanks
-Baars01-
Baars01 on Jan 22 2010, 11:13 AM said:
Hi,
Would anybody have any comments on the half-life of transfected (synthetic) pre-miRNAs and miRNA inhibitors in cells? This information is quite hard to find.
The cells I personally use are NT2/D1 and 2102Ep cells. I use the Pre-miRNAs from Ambion and miRIDIAN inhibitors from Dharmacon.
Thanks
Would anybody have any comments on the half-life of transfected (synthetic) pre-miRNAs and miRNA inhibitors in cells? This information is quite hard to find.
The cells I personally use are NT2/D1 and 2102Ep cells. I use the Pre-miRNAs from Ambion and miRIDIAN inhibitors from Dharmacon.
Thanks
Really good question...to which I'd also like to know the answer!
-miRNA man-
me too.
perhaps you can tell us from your study.
thanks
-stylothecancer-