Transfection: How long until translation starts? - (Dec/08/2009 )
does anybody of you know how long the duration from cell transfection (HeLa, 6kb plasmid) until translation start is? its because we got very different results in luciferase activity between o/n incubation and 4h incubation, respectively, before adding antibiotics. we use Roche FuGene for transfection.
I don't know the full answer, but 4 hours seems rather short for this sort of thing, at that point there will still be lots of transfection reagent/DNA lipospheres floating around waiting to hit a cell, so there is likely to be less plasmid in the cell.
In our lab we check luciferase activity usually the next day after transfection or in some cases even a day later.