luciferase - (Jul/02/2010 )
i want to ask about luciferase assay. can you share some complete site for luciferase assay starting with constructing and the appropriate controls?
Can i transfect luciferase contruct with my promoter of interest and co-transfect sirna to the cell too to knockdown a transcription factor that i suspect controls the promoter of my gene of interest? thank you
Try Promega's excellent guide on luciferase reporter systems: