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Double transfection of siRNA - (May/21/2010 )

Hi!

Is it possible to transfect cells simultaneously with two or more than two siRNAs? If yes then how do i optimize the concentrations required? How do i proceed? Plz help!

-srm mrs-

It is possible, but be cautious that when you throw in two siRNAs into a cell, they may not function equally as expected because Ago2 protein has preference over certain thermodynamically structured siRNAs. So you need proper controls,eg, combination with a control siRNA. You should also check knockdown efficiency of each siRNA used alone or in combination.

-pcrman-

pcrman on May 22 2010, 11:14 PM said:

It is possible, but be cautious that when you throw in two siRNAs into a cell, they may not function equally as expected because Ago2 protein has preference over certain thermodynamically structured siRNAs. So you need proper controls,eg, combination with a control siRNA. You should also check knockdown efficiency of each siRNA used alone or in combination.

Hi,

Thax for the reply.
So, hw do i optimize the simultaneous transfection?

-Born free-