transfection - (Mar/31/2009 )

I need to do transfection experiment to prove that a zebrafish gene is regulated by PPAR(transcription factor). I have the promoter of that gene. But i am not able to get the expression vector for the zebrafish PPAR. Can I use the human or mouse PPAR expression vector .
will it be publishable?
The cell line I am using is CHO.
is there any other way to do transfection like si RNA?

thanks
Vani

If the zebrafish PPAR gene is in high homology with human or mouse PPAR genes I think that it will give you some data.
If it works, I don't see a reason why it will not be publishable.
If it doesn’t work, you can't know if it's real or if it's a false-negative.

It's a starting point. Later try to clone the zebrafish PPAR gene.