Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
QPCR Related Discussions
  1. help with qPCR - (reply: 1)
  2. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  3. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  4. Troubleshooting qPCR standards - (reply: 1)
  5. qPCR , H&E, IHC for Influenza is not correlated - (reply: 3)
  6. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  7. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  8. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  9. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  10. qPCR and miRNA isolation - (reply: 4)
  11. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
  12. Reference gene(s) selection and validaton for qPCR - (reply: 2)
  13. Is it possible quantify dsDNA pre-sequencing with a kit in stepone plus qpcr - (reply: 2)
  14. qPCR cannot detect reaction. - (reply: 1)
  15. qRT-PCR works one day, and not the next - (reply: 1)
  16. Inter run calibration qRT-PCR - (reply: 2)
  17. qPCR amplification - (reply: 4)
  18. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
  19. how to hasten real-time PCR amplifications - (reply: 2)
  20. Different primer concentration in qPCR - (reply: 1)
  21. Problem with Real-time PCR results analysis - (reply: 1)
  22. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  23. Defining standards in qPCR softwares - (reply: 3)
  24. Having problem with primers for qPCR - (reply: 4)
  25. timecourse experiment for Western and qPCR - (reply: 2)
  26. suitable single-copy gene target for fungal qPCR - (reply: 4)
  27. qPCR of Intronless gene - (reply: 4)
  28. qPCR reference gene testing help - (reply: 4)
  29. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  30. excess rna sample affect qpcr? - (reply: 2)
  31. qpcr and sample dilution - (reply: 11)
  32. qPCR result of pooled samples - (reply: 1)
  33. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
  34. qPCR - (reply: 1)
  35. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  36. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  37. real-time pcr non reproducible - (reply: 4)
  38. Inexplicable qPCR failure in single wells - (reply: 8)
  39. analysing qpcr data and plotting standard curve - (reply: 1)
  40. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  41. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  42. CT of qPCR - (reply: 3)
  43. cDNA amount in qPCR - (reply: 2)
  44. mRNA integrity for qPCR - (reply: 6)
  45. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 6)
  46. Real-time efficiency question - (reply: 1)
  47. Selective removal of standards post qPCR Run - (reply: 1)
  48. qPCR question (no results?) - (reply: 1)
  49. >100% input in histone ChIP qPCR - (reply: 2)
  50. Sensitivity of RT-PCR and qPCR - (reply: 4)
  51. RT-qPCR primer problem - (reply: 4)
  52. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  53. Are these primer products good enough for qPCR? - (reply: 3)
  54. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  55. McrBC + qPCR : mock has higher Cts than digested? - (reply: 1)
  56. urgent: need help with qPCR statistics - (reply: 2)
  57. Dye for qPCR - (reply: 3)
  58. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  59. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
  60. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  61. reference gene for qPCR in E.coli - (reply: 1)
  62. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  63. qPCR detection - (reply: 2)
  64. qPCR with Genomic DNA - (reply: 1)
  65. How useful are Service Contracts for qPCR machines? - (reply: 2)
  66. Normalizing qPCR data - (reply: 1)
  67. High GC content RT-qPCR - please help - (reply: 3)
  68. good 16S univ. primer for qPCR? - (reply: 3)
  69. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
  70. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  71. thermocycler - real-time versus conventional? - (reply: 2)
  72. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
  73. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  74. Multiple Bands in qPCR - (reply: 4)
  75. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
  76. Reference RNA for qPCR - (reply: 3)
  77. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  78. qRT-PCR data analysis - (reply: 2)
  79. Strange RT to qPCR results with new kit - (reply: 5)
  80. qPCR: band in gel, but super high Cq? - (reply: 10)
  81. RT-qPCR normalization - (reply: 3)
  82. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  83. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
  84. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  85. qPCR: CT value of reference gene - (reply: 3)
  86. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  87. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  88. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  89. qPCR contamination problem - (reply: 5)
  90. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  91. RT-qPCR plate set up NoRT controls - (reply: 1)
  92. same primers, different product in conventional PCR and qPCR - (reply: 6)
  93. Plateau height qPCR - (reply: 3)
  94. UDG in qPCR - (reply: 1)
  95. qPCR : why substracting CT values instead of dividing ? - (reply: 3)
  96. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
  97. Experiment plan for RT-qPCR internal reference search - (reply: 3)
  98. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  99. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  100. optical qPCR plates - (reply: 1)
  101. PCR optimization: PCR vs qPCR - (reply: 4)
  102. TaqMan qPCR low-template issues - (reply: 1)
  103. qPCR standard curve slope (m) - (reply: 2)
  104. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  105. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  106. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  107. Message Amount in qPCR - (reply: 1)
  108. cDNA concentration of qPCR - (reply: 1)
  109. qPCR normalization of reference gene - (reply: 1)
  110. Desperate! qPCR standard curve problems - (reply: 2)
  111. Home made real-time PCR problem - (reply: 4)
  112. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  113. Which Brand of real-time PCR machine - (reply: 6)
  114. Weird qPCR curves - (reply: 11)
  115. Problem with New qPCR primers - help! - (reply: 6)
  116. Validation of microarray data by qPCR - (reply: 1)
  117. Help with high Ct values for Real-Time PCR - (reply: 1)
  118. Very late CP for control in rt-qPCR from RNAlater samples - (reply: 3)
  119. qPCR analysis- can I use RQ? - (reply: 1)
  120. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  121. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  122. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  123. Real-Time efficiencies and the pfaffl method - (reply: 1)
  124. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  125. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  126. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  127. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  128. qPCR Calc Help - (reply: 6)
  129. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  130. real time qRT-PCR and low expression gene - (reply: 2)
  131. Validating MeDIP by qPCR - (reply: 3)
  132. Bands seen with qPCR missing in regular PCR - (reply: 5)
  133. How to explain the difference between microarray results and qPCR data - (reply: 1)
  134. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  135. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  136. Weird qPCR curves - (reply: 4)
  137. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  138. real-time PCR after restriction enzyme digestion - (reply: 6)
  139. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  140. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  141. Help! Smear in Real-Time PCR Product - (reply: 1)
  142. Sybr green RT-qPCR primer - (reply: 1)
  143. Sybr green RT-qPCR primer - (reply: 1)
  144. running multiple qPCR with common housekeeping control - (reply: 3)
  145. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  146. Consistancy in qPCR data - (reply: 5)
  147. qPCR with low expressing genes - (reply: 1)
  148. Calculating geometric mean for real-time PCR - (reply: 5)
  149. >100% qPCR efficiency - (reply: 2)
  150. Improving qRT-PCR detection limits - (reply: 2)
  151. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  152. excel and propagation of error, qpcr - (reply: 1)
  153. Triage of miRNA targets by qPCR - (reply: 1)
  154. qPCR primer design - possible off-target (reply: 1)
  155. Primer dimer formation and real-time PCR - (reply: 7)
  156. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  157. Sequencing miRNA qRT-PCR Products - (reply: 5)
  158. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  159. Using DNA as a reference in qRT-PCR - (reply: 1)
  160. minimum replicate for qpcr? - (reply: 15)
  161. qPCR amplification interference fixed with freezing - (reply: 6)
  162. qPCR volume - Problem with volume difference and results (reply: 1)
  163. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  164. Effect of collagenase on qpcr results - (reply: 1)
  165. qPCR inhibition - (reply: 1)
  166. Primers for qPCR - (reply: 3)
  167. can RNA amplify in two-step qPCR? - (reply: 2)
  168. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  169. Using Sybr green in realtime pcr - (reply: 1)
  170. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  171. how to determine the amount of cDNA for qPCR - (reply: 5)
  172. standard curve for qPCR - (reply: 3)
  173. Standard curve for qPCR - (reply: 1)
  174. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  175. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  176. weird QPCR curves - (reply: 2)
  177. Denaturing gel electrophoresis for qPCR - (reply: 3)
  178. Long-amplicon QPCR of mtDNA - (reply: 5)
  179. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  180. Help with real-time PCR quantification of miRNA - (reply: 8)
  181. negative control in qRT-PCR - (reply: 6)
  182. Question about RealTime PCR Primer Design - (reply: 1)
  183. How to present and analyze these real-time PCR data? - (reply: 2)
  184. qPCR for transformed cells - (reply: 1)
  185. Clinical Real-time PCR assay - (reply: 1)
  186. Standard curve for real-time PCR - (reply: 2)
  187. RT-qPCR Experimental Design - How to arrange experimental replicates for treatment and control (reply: 1)
  188. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  189. Real-time qPCR data analysis - (reply: 1)
  190. Is it possible to compare data between real-time PCR plates - (reply: 1)
  191. Questions of Standards making in relative qRT-PCR - (reply: 2)
  192. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  193. Reference genes and dilutions in qPCR - (reply: 1)
  194. Issue with standard curve for qPCR for CHiP - (reply: 1)
  195. qPCR data analysis - (reply: 1)
  196. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  197. error message on qPCR mashine - (reply: 1)
  198. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  199. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  200. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  201. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  202. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
  203. qPCR Data Analysis - Software (reply: 3)
  204. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  205. Primer design for qPCR - (reply: 2)
  206. Primer design for qPCR - (reply: 1)
  207. reporting results of qPCR - (reply: 1)
  208. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
  209. question about qRT-PCR preparation - (reply: 1)
  210. qPCR efficiency calculation - (reply: 1)
  211. qPCR on chIP product - (reply: 3)
  212. QPCR/DDCT Method/ Statistics - (reply: 2)
  213. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  214. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  215. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  216. How much template DNA do you use for qPCR - (reply: 1)
  217. paranormal qpcr amplification activity - melting curves (reply: 3)
  218. General qRT-PCR question - (reply: 3)
  219. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  220. qPCR absolute/relative quantification method question - (reply: 4)
  221. Compare different qPCR runs - really need help (reply: 8)
  222. qPCR of BS DNA - (reply: 6)
  223. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  224. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  225. standards for absolute quantification qPCR - (reply: 1)
  226. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  227. qPCR, RT- Control - (reply: 2)
  228. Pre-amplification of template for qPCR - (reply: 1)
  229. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  230. conventional v real-time PCR applications - (reply: 1)
  231. different PCR primers for real-time and classic PCR - (reply: 3)
  232. qRT-PCR Inconsistency - (reply: 1)
  233. question qPCR data presentation? - (reply: 1)
  234. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  235. detect miRNAs expression by qPCR - (reply: 2)
  236. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  237. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  238. Reference for qPCR Chip? - (reply: 1)
  239. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  240. Taqman real-time PCR - Taqman and contamination (reply: 4)
  241. Data Analysis for Real-time PCR - (reply: 2)
  242. Replicate inconsistencies in qPCR - (reply: 6)
  243. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  244. Real-Time PCR - Gene expression (reply: 2)
  245. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  246. Analyzing qPCR results - (reply: 1)
  247. QPCR - Primer and Probe question - QPCR (reply: 2)
  248. qRT-PCR Product Size - (reply: 5)
  249. 18s control in qPCR - (reply: 2)
  250. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  251. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  252. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  253. qPCR analysis - (reply: 6)
  254. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  255. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  256. calibrator - what is this in qPCR (reply: 1)
  257. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  258. Realtime PCR machine - (reply: 2)
  259. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  260. home made sybr green mix for qpcr - (reply: 2)
  261. reference genes for qPCR - (reply: 3)
  262. qPCR using standard curve method - how to do correct setup? (reply: 3)
  263. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  264. Best time to isolate RNA for qPCR? - (reply: 1)
  265. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  266. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  267. Long real-time Amplicon - (reply: 2)
  268. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  269. Amplify cDNA for qRT-PCR? - (reply: 6)
  270. QPCR problem! - (reply: 9)
  271. Reference gene qPCR - (reply: 4)
  272. qRT-PCR no of cycles?? - (reply: 6)
  273. Is my primer going to work for qRT-PCR? - (reply: 6)
  274. Sybr Green I in real-time PCR reaction - (reply: 3)
  275. Measuring global methylation by QPCR - (reply: 1)
  276. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  277. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  278. Ncode qRT-PCR - (reply: 6)
  279. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  280. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  281. differently behavioring replicates in real-time PCR - (reply: 1)
  282. Endogenous control in bacterial RT-qPCR - (reply: 4)
  283. RT qPCR Newbie, Weird Results - (reply: 1)
  284. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  285. qPCR and varying cDNA concentrations - (reply: 1)
  286. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  287. qPCR and quantity of RNA - (reply: 2)
  288. How & where to prepare qPCR - (reply: 9)
  289. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  290. Normalization of qpcr data - (reply: 1)
  291. Making standard dilution for qPCR - (reply: 1)
  292. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  293. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  294. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  295. Interpreting QPCR DATA - (reply: 3)
  296. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  297. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  298. Sequencing of scarce real-time amplicons - (reply: 1)
  299. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  300. Real-Time PCR as a Microplate Reader - (reply: 1)
  301. re-amplification in real-time PCR - (reply: 1)
  302. Ct fluctuations in qPCR - (reply: 3)
  303. qPCR efficiency - how to improve (reply: 4)
  304. qPCR primer design - (reply: 5)
  305. template concentration qPCR - (reply: 1)
  306. qRT-PCR Problems - (reply: 1)
  307. qRT-PCR with DMSO - (reply: 2)
  308. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  309. Problems with qRT-PCR - (reply: 3)
  310. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  311. Understanding qPCR and gene expression changes - (reply: 10)
  312. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  313. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  314. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  315. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  316. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  317. about real time qPCR - (reply: 1)
  318. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
  319. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  320. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  321. Need helps with my QPCR result - (reply: 1)
  322. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  323. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  324. qPCR quantification of ChIP DNA - (reply: 2)
  325. Optimizing multiplex RT-qPCR - (reply: 3)