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QPCR Related Discussions
  1. Are these primer products good enough for qPCR? - (reply: 3)
  2. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  3. McrBC + qPCR : mock has higher Cts than digested? - (reply: 1)
  4. urgent: need help with qPCR statistics - (reply: 2)
  5. Dye for qPCR - (reply: 3)
  6. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  7. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
  8. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  9. reference gene for qPCR in E.coli - (reply: 1)
  10. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  11. qPCR detection - (reply: 2)
  12. qPCR with Genomic DNA - (reply: 1)
  13. How useful are Service Contracts for qPCR machines? - (reply: 2)
  14. Normalizing qPCR data - (reply: 1)
  15. High GC content RT-qPCR - please help - (reply: 3)
  16. good 16S univ. primer for qPCR? - (reply: 3)
  17. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
  18. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  19. thermocycler - real-time versus conventional? - (reply: 2)
  20. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
  21. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  22. Multiple Bands in qPCR - (reply: 4)
  23. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
  24. Reference RNA for qPCR - (reply: 2)
  25. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  26. qRT-PCR data analysis - (reply: 2)
  27. Strange RT to qPCR results with new kit - (reply: 5)
  28. qPCR: band in gel, but super high Cq? - (reply: 10)
  29. RT-qPCR normalization - (reply: 3)
  30. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  31. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
  32. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  33. qPCR: CT value of reference gene - (reply: 3)
  34. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  35. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  36. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  37. qPCR contamination problem - (reply: 5)
  38. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  39. RT-qPCR plate set up NoRT controls - (reply: 1)
  40. same primers, different product in conventional PCR and qPCR - (reply: 6)
  41. Plateau height qPCR - (reply: 3)
  42. UDG in qPCR - (reply: 1)
  43. qPCR : why substracting CT values instead of dividing ? - (reply: 3)
  44. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
  45. Experiment plan for RT-qPCR internal reference search - (reply: 3)
  46. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  47. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  48. optical qPCR plates - (reply: 1)
  49. PCR optimization: PCR vs qPCR - (reply: 4)
  50. TaqMan qPCR low-template issues - (reply: 1)
  51. qPCR standard curve slope (m) - (reply: 2)
  52. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  53. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  54. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  55. Message Amount in qPCR - (reply: 1)
  56. cDNA concentration of qPCR - (reply: 1)
  57. qPCR normalization of reference gene - (reply: 1)
  58. Desperate! qPCR standard curve problems - (reply: 2)
  59. Home made real-time PCR problem - (reply: 4)
  60. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  61. Which Brand of real-time PCR machine - (reply: 6)
  62. Weird qPCR curves - (reply: 11)
  63. Problem with New qPCR primers - help! - (reply: 6)
  64. Validation of microarray data by qPCR - (reply: 1)
  65. Help with high Ct values for Real-Time PCR - (reply: 1)
  66. Very late CP for control in rt-qPCR from RNAlater samples - (reply: 3)
  67. qPCR analysis- can I use RQ? - (reply: 1)
  68. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  69. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  70. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  71. Real-Time efficiencies and the pfaffl method - (reply: 1)
  72. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  73. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  74. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  75. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  76. qPCR Calc Help - (reply: 6)
  77. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  78. real time qRT-PCR and low expression gene - (reply: 2)
  79. Validating MeDIP by qPCR - (reply: 3)
  80. Bands seen with qPCR missing in regular PCR - (reply: 5)
  81. How to explain the difference between microarray results and qPCR data - (reply: 1)
  82. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  83. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  84. Weird qPCR curves - (reply: 4)
  85. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  86. real-time PCR after restriction enzyme digestion - (reply: 6)
  87. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  88. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  89. Help! Smear in Real-Time PCR Product - (reply: 1)
  90. Sybr green RT-qPCR primer - (reply: 1)
  91. Sybr green RT-qPCR primer - (reply: 1)
  92. running multiple qPCR with common housekeeping control - (reply: 3)
  93. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  94. Consistancy in qPCR data - (reply: 5)
  95. qPCR with low expressing genes - (reply: 1)
  96. Calculating geometric mean for real-time PCR - (reply: 5)
  97. >100% qPCR efficiency - (reply: 2)
  98. Improving qRT-PCR detection limits - (reply: 2)
  99. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  100. excel and propagation of error, qpcr - (reply: 1)
  101. Triage of miRNA targets by qPCR - (reply: 1)
  102. qPCR primer design - possible off-target (reply: 1)
  103. Primer dimer formation and real-time PCR - (reply: 7)
  104. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  105. Sequencing miRNA qRT-PCR Products - (reply: 5)
  106. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  107. Using DNA as a reference in qRT-PCR - (reply: 1)
  108. minimum replicate for qpcr? - (reply: 15)
  109. qPCR amplification interference fixed with freezing - (reply: 6)
  110. qPCR volume - Problem with volume difference and results (reply: 1)
  111. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  112. Effect of collagenase on qpcr results - (reply: 1)
  113. qPCR inhibition - (reply: 1)
  114. Primers for qPCR - (reply: 3)
  115. can RNA amplify in two-step qPCR? - (reply: 2)
  116. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  117. Using Sybr green in realtime pcr - (reply: 1)
  118. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  119. how to determine the amount of cDNA for qPCR - (reply: 5)
  120. standard curve for qPCR - (reply: 3)
  121. Standard curve for qPCR - (reply: 1)
  122. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  123. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  124. weird QPCR curves - (reply: 2)
  125. Denaturing gel electrophoresis for qPCR - (reply: 3)
  126. Long-amplicon QPCR of mtDNA - (reply: 5)
  127. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  128. Help with real-time PCR quantification of miRNA - (reply: 8)
  129. negative control in qRT-PCR - (reply: 6)
  130. Question about RealTime PCR Primer Design - (reply: 1)
  131. How to present and analyze these real-time PCR data? - (reply: 2)
  132. qPCR for transformed cells - (reply: 1)
  133. Clinical Real-time PCR assay - (reply: 1)
  134. Standard curve for real-time PCR - (reply: 2)
  135. RT-qPCR Experimental Design - How to arrange experimental replicates for treatment and control (reply: 1)
  136. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  137. Real-time qPCR data analysis - (reply: 1)
  138. Is it possible to compare data between real-time PCR plates - (reply: 1)
  139. Questions of Standards making in relative qRT-PCR - (reply: 2)
  140. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  141. Reference genes and dilutions in qPCR - (reply: 1)
  142. Issue with standard curve for qPCR for CHiP - (reply: 1)
  143. qPCR data analysis - (reply: 1)
  144. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  145. error message on qPCR mashine - (reply: 1)
  146. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  147. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 4)
  148. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  149. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  150. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
  151. qPCR Data Analysis - Software (reply: 3)
  152. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  153. Primer design for qPCR - (reply: 2)
  154. Primer design for qPCR - (reply: 1)
  155. reporting results of qPCR - (reply: 1)
  156. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
  157. question about qRT-PCR preparation - (reply: 1)
  158. qPCR efficiency calculation - (reply: 1)
  159. qPCR on chIP product - (reply: 3)
  160. QPCR/DDCT Method/ Statistics - (reply: 2)
  161. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  162. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  163. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  164. How much template DNA do you use for qPCR - (reply: 1)
  165. paranormal qpcr amplification activity - melting curves (reply: 3)
  166. General qRT-PCR question - (reply: 3)
  167. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  168. qPCR absolute/relative quantification method question - (reply: 4)
  169. Compare different qPCR runs - really need help (reply: 7)
  170. qPCR of BS DNA - (reply: 6)
  171. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  172. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  173. standards for absolute quantification qPCR - (reply: 1)
  174. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  175. qPCR, RT- Control - (reply: 2)
  176. Pre-amplification of template for qPCR - (reply: 1)
  177. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  178. conventional v real-time PCR applications - (reply: 1)
  179. different PCR primers for real-time and classic PCR - (reply: 3)
  180. qRT-PCR Inconsistency - (reply: 1)
  181. question qPCR data presentation? - (reply: 1)
  182. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  183. detect miRNAs expression by qPCR - (reply: 2)
  184. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  185. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  186. Reference for qPCR Chip? - (reply: 1)
  187. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  188. Taqman real-time PCR - Taqman and contamination (reply: 4)
  189. Data Analysis for Real-time PCR - (reply: 2)
  190. Replicate inconsistencies in qPCR - (reply: 6)
  191. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  192. Real-Time PCR - Gene expression (reply: 2)
  193. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  194. Analyzing qPCR results - (reply: 1)
  195. QPCR - Primer and Probe question - QPCR (reply: 2)
  196. qRT-PCR Product Size - (reply: 5)
  197. 18s control in qPCR - (reply: 2)
  198. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  199. microrna quantitation ---use northern blot or qrt-pcr？ - (reply: 1)
  200. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  201. qPCR analysis - (reply: 6)
  202. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  203. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  204. calibrator - what is this in qPCR (reply: 1)
  205. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  206. Realtime PCR machine - (reply: 2)
  207. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  208. home made sybr green mix for qpcr - (reply: 2)
  209. reference genes for qPCR - (reply: 3)
  210. qPCR using standard curve method - how to do correct setup? (reply: 3)
  211. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  212. Best time to isolate RNA for qPCR? - (reply: 1)
  213. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  214. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  215. Long real-time Amplicon - (reply: 2)
  216. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  217. Amplify cDNA for qRT-PCR? - (reply: 6)
  218. QPCR problem! - (reply: 9)
  219. Reference gene qPCR - (reply: 4)
  220. qRT-PCR no of cycles?? - (reply: 6)
  221. Is my primer going to work for qRT-PCR? - (reply: 5)
  222. Sybr Green I in real-time PCR reaction - (reply: 3)
  223. Measuring global methylation by QPCR - (reply: 1)
  224. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  225. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  226. Ncode qRT-PCR - (reply: 6)
  227. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  228. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  229. differently behavioring replicates in real-time PCR - (reply: 1)
  230. Endogenous control in bacterial RT-qPCR - (reply: 4)
  231. RT qPCR Newbie, Weird Results - (reply: 1)
  232. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  233. qPCR and varying cDNA concentrations - (reply: 1)
  234. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  235. qPCR and quantity of RNA - (reply: 2)
  236. How & where to prepare qPCR - (reply: 9)
  237. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  238. Normalization of qpcr data - (reply: 1)
  239. Making standard dilution for qPCR - (reply: 1)
  240. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  241. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  242. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  243. Interpreting QPCR DATA - (reply: 3)
  244. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  245. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  246. Sequencing of scarce real-time amplicons - (reply: 1)
  247. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  248. Real-Time PCR as a Microplate Reader - (reply: 1)
  249. re-amplification in real-time PCR - (reply: 1)
  250. Ct fluctuations in qPCR - (reply: 3)
  251. qPCR efficiency - how to improve (reply: 4)
  252. qPCR primer design - (reply: 5)
  253. template concentration qPCR - (reply: 1)
  254. qRT-PCR Problems - (reply: 1)
  255. qRT-PCR with DMSO - (reply: 2)
  256. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  257. Problems with qRT-PCR - (reply: 3)
  258. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  259. Understanding qPCR and gene expression changes - (reply: 10)
  260. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  261. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  262. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  263. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  264. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  265. about real time qPCR - (reply: 1)
  266. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
  267. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  268. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  269. Need helps with my QPCR result - (reply: 1)
  270. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  271. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  272. qPCR quantification of ChIP DNA - (reply: 2)
  273. Optimizing multiplex RT-qPCR - (reply: 3)