Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
QPCR Related Discussions
  1. need help setting up reverse transcriptase qPCR - (reply: 3)
  2. PCR working, qPCR is *not*. - (reply: 6)
  3. When to dilute samples for qPCR - (reply: 2)
  4. help with qPCR - (reply: 1)
  5. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  6. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  7. Troubleshooting qPCR standards - (reply: 1)
  8. qPCR , H&E, IHC for Influenza is not correlated - (reply: 3)
  9. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  10. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  11. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  12. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  13. qPCR and miRNA isolation - (reply: 4)
  14. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
  15. Reference gene(s) selection and validaton for qPCR - (reply: 2)
  16. Is it possible quantify dsDNA pre-sequencing with a kit in stepone plus qpcr - (reply: 2)
  17. qPCR cannot detect reaction. - (reply: 1)
  18. qRT-PCR works one day, and not the next - (reply: 1)
  19. Inter run calibration qRT-PCR - (reply: 2)
  20. qPCR amplification - (reply: 4)
  21. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
  22. how to hasten real-time PCR amplifications - (reply: 2)
  23. Different primer concentration in qPCR - (reply: 1)
  24. Problem with Real-time PCR results analysis - (reply: 1)
  25. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  26. Defining standards in qPCR softwares - (reply: 3)
  27. Having problem with primers for qPCR - (reply: 4)
  28. timecourse experiment for Western and qPCR - (reply: 2)
  29. suitable single-copy gene target for fungal qPCR - (reply: 4)
  30. qPCR of Intronless gene - (reply: 4)
  31. qPCR reference gene testing help - (reply: 4)
  32. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  33. excess rna sample affect qpcr? - (reply: 2)
  34. qpcr and sample dilution - (reply: 11)
  35. qPCR result of pooled samples - (reply: 1)
  36. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
  37. qPCR - (reply: 1)
  38. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  39. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  40. real-time pcr non reproducible - (reply: 4)
  41. Inexplicable qPCR failure in single wells - (reply: 10)
  42. analysing qpcr data and plotting standard curve - (reply: 1)
  43. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  44. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  45. CT of qPCR - (reply: 3)
  46. cDNA amount in qPCR - (reply: 2)
  47. mRNA integrity for qPCR - (reply: 6)
  48. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
  49. Real-time efficiency question - (reply: 1)
  50. Selective removal of standards post qPCR Run - (reply: 1)
  51. qPCR question (no results?) - (reply: 1)
  52. >100% input in histone ChIP qPCR - (reply: 2)
  53. Sensitivity of RT-PCR and qPCR - (reply: 4)
  54. RT-qPCR primer problem - (reply: 4)
  55. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  56. Are these primer products good enough for qPCR? - (reply: 3)
  57. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  58. McrBC + qPCR : mock has higher Cts than digested? - (reply: 1)
  59. urgent: need help with qPCR statistics - (reply: 2)
  60. Dye for qPCR - (reply: 3)
  61. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  62. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
  63. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  64. reference gene for qPCR in E.coli - (reply: 1)
  65. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  66. qPCR detection - (reply: 2)
  67. qPCR with Genomic DNA - (reply: 1)
  68. How useful are Service Contracts for qPCR machines? - (reply: 2)
  69. Normalizing qPCR data - (reply: 1)
  70. High GC content RT-qPCR - please help - (reply: 3)
  71. good 16S univ. primer for qPCR? - (reply: 3)
  72. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
  73. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  74. thermocycler - real-time versus conventional? - (reply: 2)
  75. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
  76. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  77. Multiple Bands in qPCR - (reply: 4)
  78. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
  79. Reference RNA for qPCR - (reply: 3)
  80. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  81. qRT-PCR data analysis - (reply: 2)
  82. Strange RT to qPCR results with new kit - (reply: 5)
  83. qPCR: band in gel, but super high Cq? - (reply: 10)
  84. RT-qPCR normalization - (reply: 3)
  85. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  86. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
  87. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  88. qPCR: CT value of reference gene - (reply: 3)
  89. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  90. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  91. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  92. qPCR contamination problem - (reply: 5)
  93. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  94. RT-qPCR plate set up NoRT controls - (reply: 1)
  95. same primers, different product in conventional PCR and qPCR - (reply: 6)
  96. Plateau height qPCR - (reply: 3)
  97. UDG in qPCR - (reply: 1)
  98. qPCR : why substracting CT values instead of dividing ? - (reply: 3)
  99. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
  100. Experiment plan for RT-qPCR internal reference search - (reply: 3)
  101. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  102. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  103. optical qPCR plates - (reply: 1)
  104. PCR optimization: PCR vs qPCR - (reply: 4)
  105. TaqMan qPCR low-template issues - (reply: 1)
  106. qPCR standard curve slope (m) - (reply: 2)
  107. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  108. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  109. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  110. Message Amount in qPCR - (reply: 1)
  111. cDNA concentration of qPCR - (reply: 1)
  112. qPCR normalization of reference gene - (reply: 1)
  113. Desperate! qPCR standard curve problems - (reply: 2)
  114. Home made real-time PCR problem - (reply: 4)
  115. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  116. Which Brand of real-time PCR machine - (reply: 6)
  117. Weird qPCR curves - (reply: 11)
  118. Problem with New qPCR primers - help! - (reply: 6)
  119. Validation of microarray data by qPCR - (reply: 1)
  120. Help with high Ct values for Real-Time PCR - (reply: 1)
  121. Very late CP for control in rt-qPCR from RNAlater samples - (reply: 3)
  122. qPCR analysis- can I use RQ? - (reply: 1)
  123. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  124. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  125. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  126. Real-Time efficiencies and the pfaffl method - (reply: 1)
  127. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  128. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  129. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  130. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  131. qPCR Calc Help - (reply: 6)
  132. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  133. real time qRT-PCR and low expression gene - (reply: 2)
  134. Validating MeDIP by qPCR - (reply: 3)
  135. Bands seen with qPCR missing in regular PCR - (reply: 5)
  136. How to explain the difference between microarray results and qPCR data - (reply: 1)
  137. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  138. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  139. Weird qPCR curves - (reply: 4)
  140. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  141. real-time PCR after restriction enzyme digestion - (reply: 6)
  142. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  143. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  144. Help! Smear in Real-Time PCR Product - (reply: 1)
  145. Sybr green RT-qPCR primer - (reply: 1)
  146. Sybr green RT-qPCR primer - (reply: 1)
  147. running multiple qPCR with common housekeeping control - (reply: 3)
  148. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  149. Consistancy in qPCR data - (reply: 5)
  150. qPCR with low expressing genes - (reply: 1)
  151. Calculating geometric mean for real-time PCR - (reply: 5)
  152. >100% qPCR efficiency - (reply: 2)
  153. Improving qRT-PCR detection limits - (reply: 2)
  154. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  155. excel and propagation of error, qpcr - (reply: 1)
  156. Triage of miRNA targets by qPCR - (reply: 1)
  157. qPCR primer design - possible off-target (reply: 1)
  158. Primer dimer formation and real-time PCR - (reply: 7)
  159. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  160. Sequencing miRNA qRT-PCR Products - (reply: 5)
  161. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  162. Using DNA as a reference in qRT-PCR - (reply: 1)
  163. minimum replicate for qpcr? - (reply: 15)
  164. qPCR amplification interference fixed with freezing - (reply: 6)
  165. qPCR volume - Problem with volume difference and results (reply: 1)
  166. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  167. Effect of collagenase on qpcr results - (reply: 1)
  168. qPCR inhibition - (reply: 1)
  169. Primers for qPCR - (reply: 3)
  170. can RNA amplify in two-step qPCR? - (reply: 2)
  171. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  172. Using Sybr green in realtime pcr - (reply: 1)
  173. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  174. how to determine the amount of cDNA for qPCR - (reply: 5)
  175. standard curve for qPCR - (reply: 3)
  176. Standard curve for qPCR - (reply: 1)
  177. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  178. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  179. weird QPCR curves - (reply: 2)
  180. Denaturing gel electrophoresis for qPCR - (reply: 3)
  181. Long-amplicon QPCR of mtDNA - (reply: 5)
  182. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  183. Help with real-time PCR quantification of miRNA - (reply: 8)
  184. negative control in qRT-PCR - (reply: 6)
  185. Question about RealTime PCR Primer Design - (reply: 1)
  186. How to present and analyze these real-time PCR data? - (reply: 2)
  187. qPCR for transformed cells - (reply: 1)
  188. Clinical Real-time PCR assay - (reply: 1)
  189. Standard curve for real-time PCR - (reply: 2)
  190. RT-qPCR Experimental Design - How to arrange experimental replicates for treatment and control (reply: 1)
  191. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  192. Real-time qPCR data analysis - (reply: 1)
  193. Is it possible to compare data between real-time PCR plates - (reply: 1)
  194. Questions of Standards making in relative qRT-PCR - (reply: 2)
  195. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  196. Reference genes and dilutions in qPCR - (reply: 1)
  197. Issue with standard curve for qPCR for CHiP - (reply: 1)
  198. qPCR data analysis - (reply: 1)
  199. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  200. error message on qPCR mashine - (reply: 1)
  201. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  202. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  203. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  204. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  205. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
  206. qPCR Data Analysis - Software (reply: 3)
  207. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  208. Primer design for qPCR - (reply: 2)
  209. Primer design for qPCR - (reply: 1)
  210. reporting results of qPCR - (reply: 1)
  211. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
  212. question about qRT-PCR preparation - (reply: 1)
  213. qPCR efficiency calculation - (reply: 1)
  214. qPCR on chIP product - (reply: 3)
  215. QPCR/DDCT Method/ Statistics - (reply: 2)
  216. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  217. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  218. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  219. How much template DNA do you use for qPCR - (reply: 1)
  220. paranormal qpcr amplification activity - melting curves (reply: 3)
  221. General qRT-PCR question - (reply: 3)
  222. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  223. qPCR absolute/relative quantification method question - (reply: 4)
  224. Compare different qPCR runs - really need help (reply: 8)
  225. qPCR of BS DNA - (reply: 6)
  226. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  227. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  228. standards for absolute quantification qPCR - (reply: 1)
  229. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  230. qPCR, RT- Control - (reply: 2)
  231. Pre-amplification of template for qPCR - (reply: 1)
  232. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  233. conventional v real-time PCR applications - (reply: 1)
  234. different PCR primers for real-time and classic PCR - (reply: 3)
  235. qRT-PCR Inconsistency - (reply: 1)
  236. question qPCR data presentation? - (reply: 1)
  237. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  238. detect miRNAs expression by qPCR - (reply: 2)
  239. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  240. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  241. Reference for qPCR Chip? - (reply: 1)
  242. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  243. Taqman real-time PCR - Taqman and contamination (reply: 4)
  244. Data Analysis for Real-time PCR - (reply: 2)
  245. Replicate inconsistencies in qPCR - (reply: 6)
  246. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  247. Real-Time PCR - Gene expression (reply: 2)
  248. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  249. Analyzing qPCR results - (reply: 1)
  250. QPCR - Primer and Probe question - QPCR (reply: 2)
  251. qRT-PCR Product Size - (reply: 5)
  252. 18s control in qPCR - (reply: 2)
  253. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  254. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  255. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  256. qPCR analysis - (reply: 6)
  257. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  258. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  259. calibrator - what is this in qPCR (reply: 1)
  260. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  261. Realtime PCR machine - (reply: 2)
  262. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  263. home made sybr green mix for qpcr - (reply: 2)
  264. reference genes for qPCR - (reply: 3)
  265. qPCR using standard curve method - how to do correct setup? (reply: 3)
  266. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  267. Best time to isolate RNA for qPCR? - (reply: 1)
  268. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  269. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  270. Long real-time Amplicon - (reply: 2)
  271. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  272. Amplify cDNA for qRT-PCR? - (reply: 6)
  273. QPCR problem! - (reply: 9)
  274. Reference gene qPCR - (reply: 4)
  275. qRT-PCR no of cycles?? - (reply: 6)
  276. Is my primer going to work for qRT-PCR? - (reply: 6)
  277. Sybr Green I in real-time PCR reaction - (reply: 3)
  278. Measuring global methylation by QPCR - (reply: 1)
  279. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  280. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  281. Ncode qRT-PCR - (reply: 6)
  282. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  283. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  284. differently behavioring replicates in real-time PCR - (reply: 1)
  285. Endogenous control in bacterial RT-qPCR - (reply: 4)
  286. RT qPCR Newbie, Weird Results - (reply: 1)
  287. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  288. qPCR and varying cDNA concentrations - (reply: 1)
  289. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  290. qPCR and quantity of RNA - (reply: 2)
  291. How & where to prepare qPCR - (reply: 9)
  292. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  293. Normalization of qpcr data - (reply: 1)
  294. Making standard dilution for qPCR - (reply: 1)
  295. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  296. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  297. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  298. Interpreting QPCR DATA - (reply: 3)
  299. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  300. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  301. Sequencing of scarce real-time amplicons - (reply: 1)
  302. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  303. Real-Time PCR as a Microplate Reader - (reply: 1)
  304. re-amplification in real-time PCR - (reply: 1)
  305. Ct fluctuations in qPCR - (reply: 3)
  306. qPCR efficiency - how to improve (reply: 4)
  307. qPCR primer design - (reply: 5)
  308. template concentration qPCR - (reply: 1)
  309. qRT-PCR Problems - (reply: 1)
  310. qRT-PCR with DMSO - (reply: 2)
  311. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  312. Problems with qRT-PCR - (reply: 3)
  313. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  314. Understanding qPCR and gene expression changes - (reply: 10)
  315. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  316. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  317. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  318. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  319. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  320. about real time qPCR - (reply: 1)
  321. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
  322. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  323. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  324. Need helps with my QPCR result - (reply: 1)
  325. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  326. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  327. qPCR quantification of ChIP DNA - (reply: 2)
  328. Optimizing multiplex RT-qPCR - (reply: 3)