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Expression Related Discussions
  1. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
  2. study protein expression in bacteria - (reply: 1)
  3. Problem with possible leaky expression in cloning - (reply: 4)
  4. Large-scale cell growth and protein expression - (reply: 1)
  5. Expression of amino acid content - (reply: 3)
  6. Mature miRNA in expression vector? - (reply: 1)
  7. consistent expression of overlarge proteins - (reply: 1)
  8. Protein expression - (reply: 1)
  9. Gene expression heat map ? - (reply: 1)
  10. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
  11. Any DNA/RNA elements I can include into transgene to delay expression? - (reply: 2)
  12. Can siRNA be used to prevent upregulation in gene expression? - (reply: 3)
  13. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  14. Loss of protein expression by transformed bacteria - (reply: 4)
  15. Protein expression database - (reply: 3)
  16. replicating a mammalian expression vector in bacteria - (reply: 3)
  17. What is best to measure protein expression: western blot or ELISA? - (reply: 4)
  18. protein expression site - (reply: 3)
  19. Expression of plant protein in Ecoli - (reply: 1)
  20. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  21. protein expression - (reply: 2)
  22. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  23. Gene expression and transcription factors - (reply: 1)
  24. Forced Expression vs. Enforced Expression - (reply: 1)
  25. Collagen binding protein expression E. coli His-tag - (reply: 1)
  26. TaqMan gene expression assays - (reply: 1)
  27. IRES in expression vector - (reply: 1)
  28. EGFP expression - (reply: 1)
  29. Determine DNA sequence expression in cell line - (reply: 1)
  30. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  31. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  32. How to increase protein expression from in vitro transcribed mRNA - (reply: 1)
  33. promoter for minimal expression of selection marker - (reply: 1)
  34. protein expression with and without induction - (reply: 1)
  35. direction of the expression units in multi-gene vectors - (reply: 1)
  36. Puromycin resistance protein expression and stability - (reply: 5)
  37. Please help me :Protein expression and purification - (reply: 1)
  38. genotype of e coli strain for protein expression - (reply: 2)
  39. Cloning worked, but the expression vector is weird - (reply: 6)
  40. No expression in stable transfected cell line - (reply: 2)
  41. Expression in different E.coli strains - (reply: 3)
  42. membrane protein expression - (reply: 2)
  43. Kozak sequence and mammalian expression vector - (reply: 3)
  44. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  45. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  46. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  47. Determining CpG methylation and effect on gene expression - (reply: 1)
  48. no protein expression - (reply: 3)
  49. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  50. Transfection but no expression - (reply: 4)
  51. Control protein for eukaryotic expression - (reply: 5)
  52. Calibrator sample has zero expression of target gene. - (reply: 1)
  53. Cloning and expression problem - (reply: 5)
  54. Protein expression - which is more sensitive/accurate? - (reply: 1)
  55. Tissue-Specific Protein Expression Database - (reply: 4)
  56. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  57. GPCR expression in HEK293 - (reply: 2)
  58. screening Protein Expression - (reply: 1)
  59. How do you compare expression in Microarray - (reply: 4)
  60. which one is better (1) Affymetrix microarrays (2) Illumina Expression BeadChips - (reply: 6)
  61. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  62. Problems in expression of target protein in E. coli - (reply: 3)
  63. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  64. Gene expression profiling with small cell number - (reply: 1)
  65. Gene expression assay with small cell number - (reply: 3)
  66. Protein expression and purification - Please help - (reply: 2)
  67. expression problem with pBAD33...similar experience? - (reply: 2)
  68. MBP-Tagged protein degrading after expression - (reply: 1)
  69. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
  70. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  71. protein expression by changing start codon - (reply: 1)
  72. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  73. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  74. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  75. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  76. Reference protein give different expression - (reply: 3)
  77. Very small peptide expression in E. coli - (reply: 2)
  78. Protein expression opposite of mRNA expression. Ideas? - (reply: 6)
  79. Gene expression database - (reply: 1)
  80. Protein expression in e.coli - (reply: 5)
  81. Gene knockdown expression levels shRNAmirs - (reply: 2)
  82. Protein Expression vector with small tag - (reply: 1)
  83. High Yield Protein Expression 10g/L - (reply: 5)
  84. E-book on recombinant protein expression - (reply: 1)
  85. Question about RNA and expression - (reply: 5)
  86. expression plasmids for tRNA - (reply: 6)
  87. a promoter question, two promoter cassetts-related expression - (reply: 2)
  88. microRNA promoter based expression vector? - (reply: 1)
  89. miRNA expression profiling - (reply: 3)
  90. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  91. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  92. CCRK (CDK20) protein expression and western blot - (reply: 7)
  93. Directions of genes expression illustrations - (reply: 5)
  94. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  95. RAW264.7 Differentiation & TRAP Expression - (reply: 5)
  96. question about protein expression normalization. - (reply: 6)
  97. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  98. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
  99. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  100. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  101. Why cmv promoter is only for transient expression? - (reply: 1)
  102. Stable protein expression in Vero cells - (reply: 2)
  103. GST-tagged protein expression problem! :-( - (reply: 4)
  104. How OD600 influence on IPTG induction and protein expression - (reply: 5)
  105. How to find all Microarray expression probes for Whole genome of Bacteria ( e.g. - (reply: 2)
  106. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  107. Multiple protein bands after expression - (reply: 6)
  108. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  109. How to measure expression the mRNA (quantitative) - (reply: 1)
  110. Expression and Lysozyme - (reply: 3)
  111. Relative fold expression and Normalization fold expression - (reply: 2)
  112. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
  113. Gene expression - (reply: 5)
  114. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  115. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  116. Protein degradation during expression - (reply: 3)
  117. Expression of insoluble protein - (reply: 4)
  118. Cloning into inducible vector but no expression - (reply: 1)
  119. ddCT issue: no endogenous expression in control. - (reply: 4)
  120. Expression level analysis at mRNA vs protein level - (reply: 6)
  121. Gene expression - (reply: 1)
  122. membrane protein expression - (reply: 1)
  123. Design primers for expression cloning - (reply: 11)
  124. Extra bands after protein expression/purification - (reply: 4)
  125. 96 well protein expression - (reply: 1)
  126. Better protein Expression? - (reply: 2)
  127. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  128. Expression of protein with pI 8.2 - (reply: 1)
  129. Methionine for protein expression - (reply: 3)
  130. Protein expression in yeast - Yeast Genotype - (reply: 1)
  131. Protein Expression problems - (reply: 4)
  132. RBS site removed in construct. Will there be expression? - (reply: 2)
  133. cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
  134. Protein tagging with GFP/effect of expression construct - (reply: 2)
  135. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  136. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
  137. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
  138. Expression in trans - (reply: 1)
  139. Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
  140. T7 Expression System - (reply: 5)
  141. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  142. GFP tagged protein expression detection - (reply: 1)
  143. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  144. Problem with expression of recombinant protein - (reply: 3)
  145. gene expression in E coli with PET21a vector - (reply: 1)
  146. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  147. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  148. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  149. mRNA increase and protein expression - (reply: 10)
  150. pcDNA 3.1 His Expression - (reply: 2)
  151. Identifying which promoters are regulating gene expression - (reply: 2)
  152. ligation of pcr product into expression vector - (reply: 4)
  153. amp plates containing xgal/iptg with expression vector - (reply: 6)
  154. protein expression level and OD600 in E.coli - (reply: 1)
  155. real time qRT-PCR and low expression gene - (reply: 2)
  156. Improving expression of recomb proteins in baculovirus - (reply: 1)
  157. P-Akt expression - (reply: 7)
  158. Increase toxic protein expression? - (reply: 1)
  159. protein expression vector.. - (reply: 4)
  160. Periplasmic Expression - Your Experience... - (reply: 2)
  161. expression problem - (reply: 2)
  162. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  163. Gene Expression Issues - (reply: 3)
  164. Caspase 3 expression - (reply: 3)
  165. Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
  166. Low or even None expression - (reply: 1)
  167. how to determine fold change of gene expression - (reply: 2)
  168. Gene expression change after mutagenesis-- please help! - (reply: 4)
  169. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  170. Upscaling protein expression in E.coli - (reply: 2)
  171. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  172. expression of promoter T7 - (reply: 6)
  173. Sigma esiRNA induces target gene expression - (reply: 1)
  174. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
  175. Protein expression vectors Please help - (reply: 2)
  176. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  177. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  178. protein level expression - (reply: 1)
  179. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  180. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  181. Low expression of pET21a construct - (reply: 6)
  182. LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
  183. Unexpected expression of my protein in HepG2 - (reply: 3)
  184. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  185. Help on mIcroRNA expression levels - concentration needed (reply: 1)
  186. Heterologous expression ABC transporters in yeast - (reply: 1)
  187. Isn't "exogenous expression" a misnomer? - (reply: 2)
  188. Protein Expression in yeast - protein not expressed (reply: 1)
  189. expression of protein - (reply: 1)
  190. insoluble protein expression help - (reply: 3)
  191. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
  192. Unstable expression in BL21 DE3 strain - (reply: 4)
  193. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
  194. FZR1 PROTEIN EXPRESSION - (reply: 1)
  195. Why shouldn't you clone using expression bacteria - (reply: 6)
  196. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  197. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  198. Antiobiotics for protein expression - (reply: 3)
  199. Lyophilization and GFP/RFP expression in bacteria - Effect of lyophilization on fluorophore expression (reply: 2)
  200. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  201. Comparison of microarray data for similarities in expression profiles - (reply: 2)
  202. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  203. Protein expression declines after overnight incubation - (reply: 4)
  204. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  205. Low miRNA expression? - (reply: 1)
  206. subcloning into bacterial expression vector - (reply: 1)
  207. cloning into F1 ori of expression vector??? - (reply: 1)
  208. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
  209. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  210. High mRNA but no protein expression - (reply: 10)
  211. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  212. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
  213. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  214. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
  215. pGEX vectors and GST expression - (reply: 3)
  216. expression vector with built in promoter - (reply: 1)
  217. expression at 30 C - (reply: 1)
  218. How Introns work in Genetric Expression - (reply: 1)
  219. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  220. problem in reporducing my result!!! - miRNA expression (reply: 1)
  221. Using CDS DNA for expression - (reply: 3)
  222. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  223. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  224. trouble with protein expression of high AT rich gene - (reply: 1)
  225. Promega IVT Problems - Low expression (reply: 1)
  226. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  227. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  228. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  229. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  230. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
  231. Protein expression - (reply: 8)
  232. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  233. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  234. Expression by ICC but not by Western-why? - (reply: 3)
  235. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  236. mammalian expression vector - (reply: 2)
  237. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  238. expression of MBP fusion protein - leaky and very low yield (reply: 2)
  239. E.coli expression - (reply: 5)
  240. Gene expression confusion - (reply: 5)
  241. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  242. miRNA 3´UTR target expression vectors - (reply: 6)
  243. very low expression of a sample vs standard curve efficiency - (reply: 1)
  244. His-tag protein expression - (reply: 1)
  245. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  246. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
  247. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
  248. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  249. e coli strain selection for protein expression - (reply: 2)
  250. Can miRNAs regulate miRNA expression - (reply: 3)
  251. Problems in upscaling protein expression - (reply: 2)
  252. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  253. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  254. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
  255. how to expression mirna in a specific tissue ? - (reply: 1)
  256. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  257. Expression of fusion proteins - (reply: 1)
  258. Problems with validating primers and low expression genes - (reply: 3)
  259. Which cell lines are best for transient transgene expression? - (reply: 3)
  260. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  261. microRNA - gene expression mechanism (reply: 1)
  262. Gene expression of methylated gene - (reply: 4)
  263. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  264. Illumina microRNA expression arrays - Problems with clustering different experiments...HELP! (reply: 5)
  265. miRNA expression - (reply: 2)
  266. shRNA expression levels - (reply: 4)
  267. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  268. detect miRNAs expression by qPCR - (reply: 2)
  269. Protein Expression in stationary phase - (reply: 11)
  270. Measuring gene expression across cell lines - (reply: 2)
  271. quantification of the expression of pre-miRNA and miRNA in plants - (reply: 1)
  272. What could be the cause for this GFP expression pattern...? - (reply: 5)
  273. Little to no expression in one group, how to analyze this? - (reply: 1)
  274. cys rich protein expression - (reply: 1)
  275. insect protein expression - (reply: 1)
  276. Stable Mammalian Expression with Retrovirus - (reply: 2)
  277. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  278. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
  279. complete gene for expression? - (reply: 2)
  280. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  281. expression of His-tagged protein in insect cells - (reply: 2)
  282. Protein expression failing - (reply: 1)
  283. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
  284. Real-Time PCR - Gene expression (reply: 2)
  285. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  286. lentiviral expression - transefection, low titier, protein expression (reply: 1)
  287. how to find out the level of expression among different transcripts of the same - (reply: 1)
  288. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  289. Validation of housekeeping gene expression - (reply: 3)
  290. problem with lentiviral expression of protein - (reply: 2)
  291. cloning of non-coding region for protein expression - protein expression (reply: 1)
  292. DE3 expression cassette - (reply: 1)
  293. Protein expression - (reply: 4)
  294. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  295. Protein expression with T7 promoter - (reply: 1)
  296. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  297. Expression problem using invitrogen pRSET C vector - (reply: 5)
  298. Protein expression using invitrogen pSecTag2 - (reply: 2)
  299. His protein expression problem - (reply: 1)
  300. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
  301. Looking for endogenus expression by using custom antibody - (reply: 5)
  302. LR reaction - Cloning into a expression vector (reply: 1)
  303. Checking Plasmid Expression - (reply: 1)
  304. how do I know if there is a miRNA modulating expression? - identification of miRNA in my system (reply: 3)
  305. Expression of a possible toxic gene? - (reply: 5)
  306. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
  307. How to compare different expression levels between genes - (reply: 1)
  308. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
  309. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  310. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  311. Surface expression by tansfection of CHO - (reply: 3)
  312. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  313. design parameters for multiple siRNA expression in vector - (reply: 4)
  314. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
  315. relative gene expression with multiple reference genes - data analysis (reply: 1)
  316. b-cell specific promoter for transgenic expression - (reply: 1)
  317. Expression of human protein in mice in-vivo - (reply: 3)
  318. Help with T7RNAP purification - Cell-free protein expression (reply: 3)
  319. Contruction of Expression Construct - (reply: 1)
  320. HeLa cells and BCL 2 expression - (reply: 1)
  321. Software for expression data relationship - (reply: 2)
  322. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
  323. What about the Baculovirus expression system? - (reply: 2)
  324. How to evaluate SV40 expression level - (reply: 4)
  325. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  326. Plasmids for protein expression in mammalian cells - (reply: 2)
  327. expression of SV40 in luciferase assay - (reply: 3)
  328. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
  329. Problem in protein expression - (reply: 2)
  330. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  331. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  332. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
  333. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  334. Improving transient CHO protein expression - (reply: 2)
  335. Expression of a GFP-tagged protein - (reply: 2)
  336. Checking For Protein Expression - (reply: 3)
  337. Transient expression vector - (reply: 11)
  338. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  339. Trypsin Expression and Purification - (reply: 3)
  340. How to check the translation? - His-tagged protein expression (reply: 8)
  341. A gateway cloning expression vector question - (reply: 1)
  342. Gene Expression analysis - (reply: 4)
  343. vivo expression technology - ivet (reply: 25)
  344. cell lines vs tissue expression - (reply: 1)
  345. Choice of expression vector - how to choose an expression vector (reply: 1)
  346. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  347. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  348. Best yeast expression system - (reply: 1)
  349. Lentivirus expression system - get no or low titer of virons (reply: 10)
  350. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  351. Transfection Expression Level Issues - (reply: 6)
  352. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
  353. distinguish protein expression from high homology - (reply: 1)
  354. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
  355. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  356. What is stringent vector? - Is it related to level of expression? (reply: 2)
  357. CMV promoter driven transgene expression - (reply: 4)
  358. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  359. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  360. Lentivirus - specific promoter expression during prodution (reply: 6)
  361. Cell culture conditions and antigen expression - (reply: 1)
  362. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  363. Gene expression in cell line - (reply: 1)
  364. in vivo expression technology - IVET (reply: 2)
  365. No protein expression - (reply: 3)
  366. truncation of protein expression - (reply: 1)
  367. Bacteria gene expression - (reply: 2)
  368. unstable protein expression - (reply: 1)
  369. primer design for pBAD topo expression kit - (reply: 2)
  370. transgenics and controls show same levels of expression!! - (reply: 5)
  371. expression in minimal media - (reply: 14)
  372. pQE vector protein expression - (reply: 3)
  373. Understanding qPCR and gene expression changes - (reply: 10)
  374. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  375. How adjacent or remote CpG affects expression? - (reply: 5)
  376. Promoters and gene expression - (reply: 3)
  377. problems with detecting protein expression - protein expression in cell (reply: 4)
  378. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
  379. Kozak consensus sequence for insect expression - (reply: 1)
  380. Expression comparision using Cp values - (reply: 1)
  381. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  382. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
  383. How to control cloned cDNA expression level in cells? - (reply: 8)
  384. Mammalian protein expression in E. coli - (reply: 1)
  385. no expression of my protein - (reply: 1)
  386. cDNA expression - (reply: 3)
  387. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
  388. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)