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Expression Related Discussions
  1. Gene expression question - (reply: 1)
  2. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
  3. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
  4. gene expression in cultured cell lines - (reply: 1)
  5. pKD1 based vector for K. lactis expression - (reply: 2)
  6. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
  7. study protein expression in bacteria - (reply: 1)
  8. Problem with possible leaky expression in cloning - (reply: 4)
  9. Large-scale cell growth and protein expression - (reply: 1)
  10. Expression of amino acid content - (reply: 3)
  11. Mature miRNA in expression vector? - (reply: 1)
  12. consistent expression of overlarge proteins - (reply: 1)
  13. Protein expression - (reply: 1)
  14. Gene expression heat map ? - (reply: 1)
  15. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
  16. Any DNA/RNA elements I can include into transgene to delay expression? - (reply: 2)
  17. Can siRNA be used to prevent upregulation in gene expression? - (reply: 3)
  18. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  19. Loss of protein expression by transformed bacteria - (reply: 4)
  20. Protein expression database - (reply: 3)
  21. replicating a mammalian expression vector in bacteria - (reply: 3)
  22. What is best to measure protein expression: western blot or ELISA? - (reply: 4)
  23. protein expression site - (reply: 3)
  24. Expression of plant protein in Ecoli - (reply: 1)
  25. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  26. protein expression - (reply: 2)
  27. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  28. Gene expression and transcription factors - (reply: 1)
  29. Forced Expression vs. Enforced Expression - (reply: 1)
  30. Collagen binding protein expression E. coli His-tag - (reply: 1)
  31. TaqMan gene expression assays - (reply: 1)
  32. IRES in expression vector - (reply: 1)
  33. EGFP expression - (reply: 1)
  34. Determine DNA sequence expression in cell line - (reply: 1)
  35. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  36. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  37. How to increase protein expression from in vitro transcribed mRNA - (reply: 1)
  38. promoter for minimal expression of selection marker - (reply: 1)
  39. protein expression with and without induction - (reply: 1)
  40. direction of the expression units in multi-gene vectors - (reply: 1)
  41. Puromycin resistance protein expression and stability - (reply: 5)
  42. Please help me :Protein expression and purification - (reply: 1)
  43. genotype of e coli strain for protein expression - (reply: 2)
  44. Cloning worked, but the expression vector is weird - (reply: 6)
  45. No expression in stable transfected cell line - (reply: 2)
  46. Expression in different E.coli strains - (reply: 3)
  47. membrane protein expression - (reply: 2)
  48. Kozak sequence and mammalian expression vector - (reply: 3)
  49. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  50. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  51. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  52. Determining CpG methylation and effect on gene expression - (reply: 1)
  53. no protein expression - (reply: 3)
  54. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  55. Transfection but no expression - (reply: 4)
  56. Control protein for eukaryotic expression - (reply: 5)
  57. Calibrator sample has zero expression of target gene. - (reply: 1)
  58. Cloning and expression problem - (reply: 5)
  59. Protein expression - which is more sensitive/accurate? - (reply: 1)
  60. Tissue-Specific Protein Expression Database - (reply: 4)
  61. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  62. GPCR expression in HEK293 - (reply: 2)
  63. screening Protein Expression - (reply: 1)
  64. How do you compare expression in Microarray - (reply: 4)
  65. which one is better (1) Affymetrix microarrays (2) Illumina Expression BeadChips - (reply: 6)
  66. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  67. Problems in expression of target protein in E. coli - (reply: 3)
  68. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  69. Gene expression profiling with small cell number - (reply: 1)
  70. Gene expression assay with small cell number - (reply: 3)
  71. Protein expression and purification - Please help - (reply: 2)
  72. expression problem with pBAD33...similar experience? - (reply: 2)
  73. MBP-Tagged protein degrading after expression - (reply: 1)
  74. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
  75. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  76. protein expression by changing start codon - (reply: 1)
  77. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  78. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  79. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  80. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  81. Reference protein give different expression - (reply: 3)
  82. Very small peptide expression in E. coli - (reply: 2)
  83. Protein expression opposite of mRNA expression. Ideas? - (reply: 6)
  84. Gene expression database - (reply: 1)
  85. Protein expression in e.coli - (reply: 5)
  86. Gene knockdown expression levels shRNAmirs - (reply: 2)
  87. Protein Expression vector with small tag - (reply: 1)
  88. High Yield Protein Expression 10g/L - (reply: 5)
  89. E-book on recombinant protein expression - (reply: 1)
  90. Question about RNA and expression - (reply: 5)
  91. expression plasmids for tRNA - (reply: 6)
  92. a promoter question, two promoter cassetts-related expression - (reply: 2)
  93. microRNA promoter based expression vector? - (reply: 1)
  94. miRNA expression profiling - (reply: 3)
  95. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  96. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  97. CCRK (CDK20) protein expression and western blot - (reply: 7)
  98. Directions of genes expression illustrations - (reply: 5)
  99. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  100. RAW264.7 Differentiation & TRAP Expression - (reply: 5)
  101. question about protein expression normalization. - (reply: 6)
  102. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  103. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
  104. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  105. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  106. Why cmv promoter is only for transient expression? - (reply: 1)
  107. Stable protein expression in Vero cells - (reply: 2)
  108. GST-tagged protein expression problem! :-( - (reply: 4)
  109. How OD600 influence on IPTG induction and protein expression - (reply: 5)
  110. How to find all Microarray expression probes for Whole genome of Bacteria ( e.g. - (reply: 2)
  111. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  112. Multiple protein bands after expression - (reply: 6)
  113. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  114. How to measure expression the mRNA (quantitative) - (reply: 1)
  115. Expression and Lysozyme - (reply: 3)
  116. Relative fold expression and Normalization fold expression - (reply: 2)
  117. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
  118. Gene expression - (reply: 5)
  119. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  120. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  121. Protein degradation during expression - (reply: 3)
  122. Expression of insoluble protein - (reply: 4)
  123. Cloning into inducible vector but no expression - (reply: 1)
  124. ddCT issue: no endogenous expression in control. - (reply: 4)
  125. Expression level analysis at mRNA vs protein level - (reply: 6)
  126. Gene expression - (reply: 1)
  127. membrane protein expression - (reply: 1)
  128. Design primers for expression cloning - (reply: 11)
  129. Extra bands after protein expression/purification - (reply: 4)
  130. 96 well protein expression - (reply: 1)
  131. Better protein Expression? - (reply: 2)
  132. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  133. Expression of protein with pI 8.2 - (reply: 1)
  134. Methionine for protein expression - (reply: 3)
  135. Protein expression in yeast - Yeast Genotype - (reply: 1)
  136. Protein Expression problems - (reply: 4)
  137. RBS site removed in construct. Will there be expression? - (reply: 2)
  138. cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
  139. Protein tagging with GFP/effect of expression construct - (reply: 2)
  140. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  141. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
  142. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
  143. Expression in trans - (reply: 1)
  144. Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
  145. T7 Expression System - (reply: 5)
  146. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  147. GFP tagged protein expression detection - (reply: 1)
  148. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  149. Problem with expression of recombinant protein - (reply: 3)
  150. gene expression in E coli with PET21a vector - (reply: 1)
  151. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  152. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  153. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  154. mRNA increase and protein expression - (reply: 10)
  155. pcDNA 3.1 His Expression - (reply: 2)
  156. Identifying which promoters are regulating gene expression - (reply: 2)
  157. ligation of pcr product into expression vector - (reply: 4)
  158. amp plates containing xgal/iptg with expression vector - (reply: 6)
  159. protein expression level and OD600 in E.coli - (reply: 1)
  160. real time qRT-PCR and low expression gene - (reply: 2)
  161. Improving expression of recomb proteins in baculovirus - (reply: 1)
  162. P-Akt expression - (reply: 7)
  163. Increase toxic protein expression? - (reply: 1)
  164. protein expression vector.. - (reply: 4)
  165. Periplasmic Expression - Your Experience... - (reply: 2)
  166. expression problem - (reply: 2)
  167. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  168. Gene Expression Issues - (reply: 3)
  169. Caspase 3 expression - (reply: 3)
  170. Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
  171. Low or even None expression - (reply: 1)
  172. how to determine fold change of gene expression - (reply: 2)
  173. Gene expression change after mutagenesis-- please help! - (reply: 4)
  174. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  175. Upscaling protein expression in E.coli - (reply: 2)
  176. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  177. expression of promoter T7 - (reply: 6)
  178. Sigma esiRNA induces target gene expression - (reply: 1)
  179. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
  180. Protein expression vectors Please help - (reply: 2)
  181. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  182. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  183. protein level expression - (reply: 1)
  184. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  185. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  186. Low expression of pET21a construct - (reply: 6)
  187. LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
  188. Unexpected expression of my protein in HepG2 - (reply: 3)
  189. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  190. Help on mIcroRNA expression levels - concentration needed (reply: 1)
  191. Heterologous expression ABC transporters in yeast - (reply: 1)
  192. Isn't "exogenous expression" a misnomer? - (reply: 2)
  193. Protein Expression in yeast - protein not expressed (reply: 1)
  194. expression of protein - (reply: 1)
  195. insoluble protein expression help - (reply: 3)
  196. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
  197. Unstable expression in BL21 DE3 strain - (reply: 4)
  198. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
  199. FZR1 PROTEIN EXPRESSION - (reply: 1)
  200. Why shouldn't you clone using expression bacteria - (reply: 6)
  201. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  202. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  203. Antiobiotics for protein expression - (reply: 3)
  204. Lyophilization and GFP/RFP expression in bacteria - Effect of lyophilization on fluorophore expression (reply: 2)
  205. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  206. Comparison of microarray data for similarities in expression profiles - (reply: 2)
  207. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  208. Protein expression declines after overnight incubation - (reply: 4)
  209. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  210. Low miRNA expression? - (reply: 1)
  211. subcloning into bacterial expression vector - (reply: 1)
  212. cloning into F1 ori of expression vector??? - (reply: 1)
  213. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
  214. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  215. High mRNA but no protein expression - (reply: 10)
  216. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  217. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
  218. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  219. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
  220. pGEX vectors and GST expression - (reply: 3)
  221. expression vector with built in promoter - (reply: 1)
  222. expression at 30 C - (reply: 1)
  223. How Introns work in Genetric Expression - (reply: 1)
  224. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  225. problem in reporducing my result!!! - miRNA expression (reply: 1)
  226. Using CDS DNA for expression - (reply: 3)
  227. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  228. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  229. trouble with protein expression of high AT rich gene - (reply: 1)
  230. Promega IVT Problems - Low expression (reply: 1)
  231. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  232. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  233. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  234. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  235. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
  236. Protein expression - (reply: 8)
  237. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  238. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  239. Expression by ICC but not by Western-why? - (reply: 3)
  240. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  241. mammalian expression vector - (reply: 2)
  242. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  243. expression of MBP fusion protein - leaky and very low yield (reply: 2)
  244. E.coli expression - (reply: 5)
  245. Gene expression confusion - (reply: 5)
  246. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  247. miRNA 3´UTR target expression vectors - (reply: 6)
  248. very low expression of a sample vs standard curve efficiency - (reply: 1)
  249. His-tag protein expression - (reply: 1)
  250. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  251. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
  252. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
  253. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  254. e coli strain selection for protein expression - (reply: 2)
  255. Can miRNAs regulate miRNA expression - (reply: 3)
  256. Problems in upscaling protein expression - (reply: 2)
  257. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  258. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  259. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
  260. how to expression mirna in a specific tissue ? - (reply: 1)
  261. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  262. Expression of fusion proteins - (reply: 1)
  263. Problems with validating primers and low expression genes - (reply: 3)
  264. Which cell lines are best for transient transgene expression? - (reply: 3)
  265. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  266. microRNA - gene expression mechanism (reply: 1)
  267. Gene expression of methylated gene - (reply: 4)
  268. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  269. Illumina microRNA expression arrays - Problems with clustering different experiments...HELP! (reply: 5)
  270. miRNA expression - (reply: 2)
  271. shRNA expression levels - (reply: 4)
  272. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  273. detect miRNAs expression by qPCR - (reply: 2)
  274. Protein Expression in stationary phase - (reply: 11)
  275. Measuring gene expression across cell lines - (reply: 2)
  276. quantification of the expression of pre-miRNA and miRNA in plants - (reply: 1)
  277. What could be the cause for this GFP expression pattern...? - (reply: 5)
  278. Little to no expression in one group, how to analyze this? - (reply: 1)
  279. cys rich protein expression - (reply: 1)
  280. insect protein expression - (reply: 1)
  281. Stable Mammalian Expression with Retrovirus - (reply: 2)
  282. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  283. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
  284. complete gene for expression? - (reply: 2)
  285. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  286. expression of His-tagged protein in insect cells - (reply: 2)
  287. Protein expression failing - (reply: 1)
  288. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
  289. Real-Time PCR - Gene expression (reply: 2)
  290. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  291. lentiviral expression - transefection, low titier, protein expression (reply: 1)
  292. how to find out the level of expression among different transcripts of the same - (reply: 1)
  293. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  294. Validation of housekeeping gene expression - (reply: 3)
  295. problem with lentiviral expression of protein - (reply: 2)
  296. cloning of non-coding region for protein expression - protein expression (reply: 1)
  297. DE3 expression cassette - (reply: 1)
  298. Protein expression - (reply: 4)
  299. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  300. Protein expression with T7 promoter - (reply: 1)
  301. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  302. Expression problem using invitrogen pRSET C vector - (reply: 5)
  303. Protein expression using invitrogen pSecTag2 - (reply: 2)
  304. His protein expression problem - (reply: 1)
  305. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
  306. Looking for endogenus expression by using custom antibody - (reply: 5)
  307. LR reaction - Cloning into a expression vector (reply: 1)
  308. Checking Plasmid Expression - (reply: 1)
  309. how do I know if there is a miRNA modulating expression? - identification of miRNA in my system (reply: 3)
  310. Expression of a possible toxic gene? - (reply: 5)
  311. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
  312. How to compare different expression levels between genes - (reply: 1)
  313. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
  314. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  315. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  316. Surface expression by tansfection of CHO - (reply: 3)
  317. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  318. design parameters for multiple siRNA expression in vector - (reply: 4)
  319. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
  320. relative gene expression with multiple reference genes - data analysis (reply: 1)
  321. b-cell specific promoter for transgenic expression - (reply: 1)
  322. Expression of human protein in mice in-vivo - (reply: 3)
  323. Help with T7RNAP purification - Cell-free protein expression (reply: 3)
  324. Contruction of Expression Construct - (reply: 1)
  325. HeLa cells and BCL 2 expression - (reply: 1)
  326. Software for expression data relationship - (reply: 2)
  327. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
  328. What about the Baculovirus expression system? - (reply: 2)
  329. How to evaluate SV40 expression level - (reply: 4)
  330. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  331. Plasmids for protein expression in mammalian cells - (reply: 2)
  332. expression of SV40 in luciferase assay - (reply: 3)
  333. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
  334. Problem in protein expression - (reply: 2)
  335. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  336. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  337. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
  338. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  339. Improving transient CHO protein expression - (reply: 2)
  340. Expression of a GFP-tagged protein - (reply: 2)
  341. Checking For Protein Expression - (reply: 3)
  342. Transient expression vector - (reply: 11)
  343. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  344. Trypsin Expression and Purification - (reply: 3)
  345. How to check the translation? - His-tagged protein expression (reply: 8)
  346. A gateway cloning expression vector question - (reply: 1)
  347. Gene Expression analysis - (reply: 4)
  348. vivo expression technology - ivet (reply: 25)
  349. cell lines vs tissue expression - (reply: 1)
  350. Choice of expression vector - how to choose an expression vector (reply: 1)
  351. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  352. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  353. Best yeast expression system - (reply: 1)
  354. Lentivirus expression system - get no or low titer of virons (reply: 10)
  355. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  356. Transfection Expression Level Issues - (reply: 6)
  357. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
  358. distinguish protein expression from high homology - (reply: 1)
  359. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
  360. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  361. What is stringent vector? - Is it related to level of expression? (reply: 2)
  362. CMV promoter driven transgene expression - (reply: 4)
  363. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  364. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  365. Lentivirus - specific promoter expression during prodution (reply: 6)
  366. Cell culture conditions and antigen expression - (reply: 1)
  367. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  368. Gene expression in cell line - (reply: 1)
  369. in vivo expression technology - IVET (reply: 2)
  370. No protein expression - (reply: 3)
  371. truncation of protein expression - (reply: 1)
  372. Bacteria gene expression - (reply: 2)
  373. unstable protein expression - (reply: 1)
  374. primer design for pBAD topo expression kit - (reply: 2)
  375. transgenics and controls show same levels of expression!! - (reply: 5)
  376. expression in minimal media - (reply: 14)
  377. pQE vector protein expression - (reply: 3)
  378. Understanding qPCR and gene expression changes - (reply: 10)
  379. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  380. How adjacent or remote CpG affects expression? - (reply: 5)
  381. Promoters and gene expression - (reply: 3)
  382. problems with detecting protein expression - protein expression in cell (reply: 4)
  383. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
  384. Kozak consensus sequence for insect expression - (reply: 1)
  385. Expression comparision using Cp values - (reply: 1)
  386. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  387. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
  388. How to control cloned cDNA expression level in cells? - (reply: 8)
  389. Mammalian protein expression in E. coli - (reply: 1)
  390. no expression of my protein - (reply: 1)
  391. cDNA expression - (reply: 3)
  392. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
  393. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)