Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Expression Related Discussions
  1. MDA-kb2 cells - problem with luciferase expression - (reply: 4)
  2. Loss of protein expression by transformed bacteria - (reply: 3)
  3. Protein expression database - (reply: 3)
  4. replicating a mammalian expression vector in bacteria - (reply: 3)
  5. What is best to measure protein expression: western blot or ELISA? - (reply: 2)
  6. protein expression site - (reply: 3)
  7. Expression of plant protein in Ecoli - (reply: 1)
  8. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  9. protein expression - (reply: 2)
  10. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  11. Gene expression and transcription factors - (reply: 1)
  12. Forced Expression vs. Enforced Expression - (reply: 1)
  13. Collagen binding protein expression E. coli His-tag - (reply: 1)
  14. TaqMan gene expression assays - (reply: 1)
  15. IRES in expression vector - (reply: 1)
  16. EGFP expression - (reply: 1)
  17. Determine DNA sequence expression in cell line - (reply: 1)
  18. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  19. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  20. How to increase protein expression from in vitro transcribed mRNA - (reply: 1)
  21. promoter for minimal expression of selection marker - (reply: 1)
  22. protein expression with and without induction - (reply: 1)
  23. direction of the expression units in multi-gene vectors - (reply: 1)
  24. Puromycin resistance protein expression and stability - (reply: 5)
  25. Please help me :Protein expression and purification - (reply: 1)
  26. genotype of e coli strain for protein expression - (reply: 2)
  27. Cloning worked, but the expression vector is weird - (reply: 6)
  28. No expression in stable transfected cell line - (reply: 2)
  29. Expression in different E.coli strains - (reply: 3)
  30. membrane protein expression - (reply: 2)
  31. Kozak sequence and mammalian expression vector - (reply: 3)
  32. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  33. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  34. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  35. Determining CpG methylation and effect on gene expression - (reply: 1)
  36. no protein expression - (reply: 3)
  37. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  38. Transfection but no expression - (reply: 4)
  39. Control protein for eukaryotic expression - (reply: 5)
  40. Calibrator sample has zero expression of target gene. - (reply: 1)
  41. Cloning and expression problem - (reply: 5)
  42. Protein expression - which is more sensitive/accurate? - (reply: 1)
  43. Tissue-Specific Protein Expression Database - (reply: 4)
  44. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  45. GPCR expression in HEK293 - (reply: 2)
  46. screening Protein Expression - (reply: 1)
  47. How do you compare expression in Microarray - (reply: 4)
  48. which one is better (1) Affymetrix microarrays (2) Illumina Expression BeadChips - (reply: 6)
  49. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  50. Problems in expression of target protein in E. coli - (reply: 3)
  51. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  52. Gene expression profiling with small cell number - (reply: 1)
  53. Gene expression assay with small cell number - (reply: 3)
  54. Protein expression and purification - Please help - (reply: 2)
  55. expression problem with pBAD33...similar experience? - (reply: 2)
  56. MBP-Tagged protein degrading after expression - (reply: 1)
  57. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
  58. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  59. protein expression by changing start codon - (reply: 1)
  60. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  61. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  62. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  63. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  64. Reference protein give different expression - (reply: 3)
  65. Very small peptide expression in E. coli - (reply: 2)
  66. Protein expression opposite of mRNA expression. Ideas? - (reply: 6)
  67. Gene expression database - (reply: 1)
  68. Protein expression in e.coli - (reply: 5)
  69. Gene knockdown expression levels shRNAmirs - (reply: 2)
  70. Protein Expression vector with small tag - (reply: 1)
  71. High Yield Protein Expression 10g/L - (reply: 5)
  72. E-book on recombinant protein expression - (reply: 1)
  73. Question about RNA and expression - (reply: 5)
  74. expression plasmids for tRNA - (reply: 6)
  75. a promoter question, two promoter cassetts-related expression - (reply: 2)
  76. microRNA promoter based expression vector? - (reply: 1)
  77. miRNA expression profiling - (reply: 3)
  78. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  79. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
  80. CCRK (CDK20) protein expression and western blot - (reply: 7)
  81. Directions of genes expression illustrations - (reply: 5)
  82. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  83. RAW264.7 Differentiation & TRAP Expression - (reply: 4)
  84. question about protein expression normalization. - (reply: 6)
  85. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  86. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
  87. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  88. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  89. Why cmv promoter is only for transient expression? - (reply: 1)
  90. Stable protein expression in Vero cells - (reply: 2)
  91. GST-tagged protein expression problem! :-( - (reply: 4)
  92. How OD600 influence on IPTG induction and protein expression - (reply: 5)
  93. How to find all Microarray expression probes for Whole genome of Bacteria ( e.g. - (reply: 2)
  94. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  95. Multiple protein bands after expression - (reply: 6)
  96. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  97. How to measure expression the mRNA (quantitative) - (reply: 1)
  98. Expression and Lysozyme - (reply: 3)
  99. Relative fold expression and Normalization fold expression - (reply: 2)
  100. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
  101. Gene expression - (reply: 5)
  102. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  103. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  104. Protein degradation during expression - (reply: 3)
  105. Expression of insoluble protein - (reply: 4)
  106. Cloning into inducible vector but no expression - (reply: 1)
  107. ddCT issue: no endogenous expression in control. - (reply: 4)
  108. Expression level analysis at mRNA vs protein level - (reply: 6)
  109. Gene expression - (reply: 1)
  110. membrane protein expression - (reply: 1)
  111. Design primers for expression cloning - (reply: 11)
  112. Extra bands after protein expression/purification - (reply: 4)
  113. 96 well protein expression - (reply: 1)
  114. Better protein Expression? - (reply: 2)
  115. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  116. Expression of protein with pI 8.2 - (reply: 1)
  117. Methionine for protein expression - (reply: 3)
  118. Protein expression in yeast - Yeast Genotype - (reply: 1)
  119. Protein Expression problems - (reply: 4)
  120. RBS site removed in construct. Will there be expression? - (reply: 2)
  121. cDNA or genomic DNA for over expression in Arabidopsis - (reply: 1)
  122. Protein tagging with GFP/effect of expression construct - (reply: 2)
  123. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  124. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
  125. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
  126. Expression in trans - (reply: 1)
  127. Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
  128. T7 Expression System - (reply: 5)
  129. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  130. GFP tagged protein expression detection - (reply: 1)
  131. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  132. Problem with expression of recombinant protein - (reply: 3)
  133. gene expression in E coli with PET21a vector - (reply: 1)
  134. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  135. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  136. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  137. mRNA increase and protein expression - (reply: 10)
  138. pcDNA 3.1 His Expression - (reply: 2)
  139. Identifying which promoters are regulating gene expression - (reply: 2)
  140. ligation of pcr product into expression vector - (reply: 4)
  141. amp plates containing xgal/iptg with expression vector - (reply: 6)
  142. protein expression level and OD600 in E.coli - (reply: 1)
  143. real time qRT-PCR and low expression gene - (reply: 2)
  144. Improving expression of recomb proteins in baculovirus - (reply: 1)
  145. P-Akt expression - (reply: 7)
  146. Increase toxic protein expression? - (reply: 1)
  147. protein expression vector.. - (reply: 4)
  148. Periplasmic Expression - Your Experience... - (reply: 2)
  149. expression problem - (reply: 2)
  150. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  151. Gene Expression Issues - (reply: 3)
  152. Caspase 3 expression - (reply: 3)
  153. Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
  154. Low or even None expression - (reply: 1)
  155. how to determine fold change of gene expression - (reply: 2)
  156. Gene expression change after mutagenesis-- please help! - (reply: 4)
  157. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  158. Upscaling protein expression in E.coli - (reply: 2)
  159. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  160. expression of promoter T7 - (reply: 6)
  161. Sigma esiRNA induces target gene expression - (reply: 1)
  162. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
  163. Protein expression vectors Please help - (reply: 2)
  164. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  165. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  166. protein level expression - (reply: 1)
  167. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  168. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  169. Low expression of pET21a construct - (reply: 6)
  170. LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
  171. Unexpected expression of my protein in HepG2 - (reply: 3)
  172. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  173. Help on mIcroRNA expression levels - concentration needed (reply: 1)
  174. Heterologous expression ABC transporters in yeast - (reply: 1)
  175. Isn't "exogenous expression" a misnomer? - (reply: 2)
  176. Protein Expression in yeast - protein not expressed (reply: 1)
  177. expression of protein - (reply: 1)
  178. insoluble protein expression help - (reply: 3)
  179. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
  180. Unstable expression in BL21 DE3 strain - (reply: 4)
  181. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
  182. FZR1 PROTEIN EXPRESSION - (reply: 1)
  183. Why shouldn't you clone using expression bacteria - (reply: 6)
  184. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  185. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  186. Antiobiotics for protein expression - (reply: 3)
  187. Lyophilization and GFP/RFP expression in bacteria - Effect of lyophilization on fluorophore expression (reply: 2)
  188. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  189. Comparison of microarray data for similarities in expression profiles - (reply: 2)
  190. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  191. Protein expression declines after overnight incubation - (reply: 4)
  192. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
  193. Low miRNA expression? - (reply: 1)
  194. subcloning into bacterial expression vector - (reply: 1)
  195. cloning into F1 ori of expression vector??? - (reply: 1)
  196. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
  197. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  198. High mRNA but no protein expression - (reply: 10)
  199. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  200. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
  201. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  202. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
  203. pGEX vectors and GST expression - (reply: 3)
  204. expression vector with built in promoter - (reply: 1)
  205. expression at 30 C - (reply: 1)
  206. How Introns work in Genetric Expression - (reply: 1)
  207. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  208. problem in reporducing my result!!! - miRNA expression (reply: 1)
  209. Using CDS DNA for expression - (reply: 3)
  210. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  211. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  212. trouble with protein expression of high AT rich gene - (reply: 1)
  213. Promega IVT Problems - Low expression (reply: 1)
  214. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  215. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  216. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  217. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  218. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
  219. Protein expression - (reply: 8)
  220. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  221. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  222. Expression by ICC but not by Western-why? - (reply: 3)
  223. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  224. mammalian expression vector - (reply: 2)
  225. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  226. expression of MBP fusion protein - leaky and very low yield (reply: 2)
  227. E.coli expression - (reply: 5)
  228. Gene expression confusion - (reply: 5)
  229. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  230. miRNA 3´UTR target expression vectors - (reply: 6)
  231. very low expression of a sample vs standard curve efficiency - (reply: 1)
  232. His-tag protein expression - (reply: 1)
  233. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  234. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
  235. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
  236. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  237. e coli strain selection for protein expression - (reply: 2)
  238. Can miRNAs regulate miRNA expression - (reply: 3)
  239. Problems in upscaling protein expression - (reply: 2)
  240. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  241. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  242. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
  243. how to expression mirna in a specific tissue ? - (reply: 1)
  244. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
  245. Expression of fusion proteins - (reply: 1)
  246. Problems with validating primers and low expression genes - (reply: 3)
  247. Which cell lines are best for transient transgene expression? - (reply: 3)
  248. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  249. microRNA - gene expression mechanism (reply: 1)
  250. Gene expression of methylated gene - (reply: 4)
  251. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  252. Illumina microRNA expression arrays - Problems with clustering different experiments...HELP! (reply: 5)
  253. miRNA expression - (reply: 2)
  254. shRNA expression levels - (reply: 4)
  255. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  256. detect miRNAs expression by qPCR - (reply: 2)
  257. Protein Expression in stationary phase - (reply: 11)
  258. Measuring gene expression across cell lines - (reply: 2)
  259. quantification of the expression of pre-miRNA and miRNA in plants - (reply: 1)
  260. What could be the cause for this GFP expression pattern...? - (reply: 5)
  261. Little to no expression in one group, how to analyze this? - (reply: 1)
  262. cys rich protein expression - (reply: 1)
  263. insect protein expression - (reply: 1)
  264. Stable Mammalian Expression with Retrovirus - (reply: 2)
  265. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  266. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
  267. complete gene for expression? - (reply: 2)
  268. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  269. expression of His-tagged protein in insect cells - (reply: 2)
  270. Protein expression failing - (reply: 1)
  271. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
  272. Real-Time PCR - Gene expression (reply: 2)
  273. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  274. lentiviral expression - transefection, low titier, protein expression (reply: 1)
  275. how to find out the level of expression among different transcripts of the same - (reply: 1)
  276. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  277. Validation of housekeeping gene expression - (reply: 3)
  278. problem with lentiviral expression of protein - (reply: 2)
  279. cloning of non-coding region for protein expression - protein expression (reply: 1)
  280. DE3 expression cassette - (reply: 1)
  281. Protein expression - (reply: 4)
  282. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  283. Protein expression with T7 promoter - (reply: 1)
  284. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  285. Expression problem using invitrogen pRSET C vector - (reply: 5)
  286. Protein expression using invitrogen pSecTag2 - (reply: 2)
  287. His protein expression problem - (reply: 1)
  288. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
  289. Looking for endogenus expression by using custom antibody - (reply: 5)
  290. LR reaction - Cloning into a expression vector (reply: 1)
  291. Checking Plasmid Expression - (reply: 1)
  292. how do I know if there is a miRNA modulating expression? - identification of miRNA in my system (reply: 3)
  293. Expression of a possible toxic gene? - (reply: 5)
  294. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
  295. How to compare different expression levels between genes - (reply: 1)
  296. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
  297. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  298. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  299. Surface expression by tansfection of CHO - (reply: 3)
  300. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  301. design parameters for multiple siRNA expression in vector - (reply: 4)
  302. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
  303. relative gene expression with multiple reference genes - data analysis (reply: 1)
  304. b-cell specific promoter for transgenic expression - (reply: 1)
  305. Expression of human protein in mice in-vivo - (reply: 3)
  306. Help with T7RNAP purification - Cell-free protein expression (reply: 3)
  307. Contruction of Expression Construct - (reply: 1)
  308. HeLa cells and BCL 2 expression - (reply: 1)
  309. Software for expression data relationship - (reply: 2)
  310. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
  311. What about the Baculovirus expression system? - (reply: 2)
  312. How to evaluate SV40 expression level - (reply: 4)
  313. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  314. Plasmids for protein expression in mammalian cells - (reply: 2)
  315. expression of SV40 in luciferase assay - (reply: 3)
  316. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
  317. Problem in protein expression - (reply: 2)
  318. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  319. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  320. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
  321. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  322. Improving transient CHO protein expression - (reply: 2)
  323. Expression of a GFP-tagged protein - (reply: 2)
  324. Checking For Protein Expression - (reply: 3)
  325. Transient expression vector - (reply: 11)
  326. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  327. Trypsin Expression and Purification - (reply: 3)
  328. How to check the translation? - His-tagged protein expression (reply: 8)
  329. A gateway cloning expression vector question - (reply: 1)
  330. Gene Expression analysis - (reply: 4)
  331. vivo expression technology - ivet (reply: 25)
  332. cell lines vs tissue expression - (reply: 1)
  333. Choice of expression vector - how to choose an expression vector (reply: 1)
  334. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  335. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  336. Best yeast expression system - (reply: 1)
  337. Lentivirus expression system - get no or low titer of virons (reply: 10)
  338. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  339. Transfection Expression Level Issues - (reply: 6)
  340. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
  341. distinguish protein expression from high homology - (reply: 1)
  342. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
  343. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  344. What is stringent vector? - Is it related to level of expression? (reply: 2)
  345. CMV promoter driven transgene expression - (reply: 4)
  346. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  347. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  348. Lentivirus - specific promoter expression during prodution (reply: 6)
  349. Cell culture conditions and antigen expression - (reply: 1)
  350. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  351. Gene expression in cell line - (reply: 1)
  352. in vivo expression technology - IVET (reply: 2)
  353. No protein expression - (reply: 3)
  354. truncation of protein expression - (reply: 1)
  355. Bacteria gene expression - (reply: 2)
  356. unstable protein expression - (reply: 1)
  357. primer design for pBAD topo expression kit - (reply: 2)
  358. transgenics and controls show same levels of expression!! - (reply: 5)
  359. expression in minimal media - (reply: 14)
  360. pQE vector protein expression - (reply: 3)
  361. Understanding qPCR and gene expression changes - (reply: 10)
  362. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  363. How adjacent or remote CpG affects expression? - (reply: 5)
  364. Promoters and gene expression - (reply: 3)
  365. problems with detecting protein expression - protein expression in cell (reply: 4)
  366. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
  367. Kozak consensus sequence for insect expression - (reply: 1)
  368. Expression comparision using Cp values - (reply: 1)
  369. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  370. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
  371. How to control cloned cDNA expression level in cells? - (reply: 8)
  372. Mammalian protein expression in E. coli - (reply: 1)
  373. no expression of my protein - (reply: 1)
  374. cDNA expression - (reply: 3)
  375. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
  376. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)