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how to expression mirna in a specific tissue ? - (May/21/2010 )

I want to express a mirna in the liver of zebrafish ?
How to build this kind of vector?
thank you


You will need to clone the precursor miRNA sequence (the pre-miRNA sequence plus 100-200 bp flanking sequences on both sides) into a expression vector and the transcription of the miRNA in the vector should be driven by a tissue (liver) specific promoter.