 Isolated nuclei and trypan blue, Silly question .......... |
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 Jul 2 2009, 12:43 AM
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#1
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member  Group: Members Posts: 2 Joined: 2-July 09 Member No.: 10676  |
Hi all,
Long time listener, first time caller! I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells? Many thanks! |
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Jul 2 2009, 04:14 PM
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#2
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 Hmmm, I think it's working Group: Active Members Posts: 818 Joined: 21-July 04 Member No.: 3573 |
They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.
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Jul 4 2009, 09:42 AM
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#3
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member Group: Members Posts: 2 Joined: 2-July 09 Member No.: 10676 |
QUOTE (bob1 @ Jul 3 2009, 01:14 AM)  They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes. Thanks for that! I'll take a look .......... |
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