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1. pQE30 in E.coli - (reply: 1)
2. no expression of my target protein - (reply: 3)
3. His-tag purified protein is yellow! - (reply: 5)
4. has anyone used PopCulture from Novagen? - (reply: 1)
5. AKTA purifier Prgramming - (reply: 2)
6. unexpected 40kDa band -E.coli protein expression - (reply: 3)
7. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
8. Storage of samples for phosphorilated proteins - (reply: 1)
9. Aromatic Aminoacids - (reply: 1)
10. Troubles with expression proteins - (reply: 5)
11. protein on membrane - (reply: 1)
12. E.Coli ceased to express my target protein - (reply: 5)
13. protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
14. problem with solubilizing protein in formic acid - (reply: 1)
15. storing dialysis tube(bag) - (reply: 2)
16. treating dialysis bag with EDTA - (reply: 1)
17. HIS TAG Protein Estimation - (reply: 4)
18. DEAE PREPARATION - (reply: 1)
19. His-TAG purification - (reply: 1)
20. Problem with GST tagged baculovirus protein - (reply: 1)
21. sandwich Ni column - (reply: 1)
22. protein solubility - (reply: 2)
23. native purification of His-tagged proteins from E. coli? - (reply: 1)
24. GST tagged protein purification - (reply: 8)
25. bacterial growth arrest after IPTG induction - (reply: 2)
26. Protein insolubility problem after bacterial induction - (reply: 2)
27. what cells to use for expression of pGEX3? - (reply: 1)
28. Analysis protein expression - (reply: 1)
29. Protein elution problems - (reply: 2)
30. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
2. no expression of my target protein - (reply: 3)
3. His-tag purified protein is yellow! - (reply: 5)
4. has anyone used PopCulture from Novagen? - (reply: 1)
5. AKTA purifier Prgramming - (reply: 2)
6. unexpected 40kDa band -E.coli protein expression - (reply: 3)
7. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
8. Storage of samples for phosphorilated proteins - (reply: 1)
9. Aromatic Aminoacids - (reply: 1)
10. Troubles with expression proteins - (reply: 5)
11. protein on membrane - (reply: 1)
12. E.Coli ceased to express my target protein - (reply: 5)
13. protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
14. problem with solubilizing protein in formic acid - (reply: 1)
15. storing dialysis tube(bag) - (reply: 2)
16. treating dialysis bag with EDTA - (reply: 1)
17. HIS TAG Protein Estimation - (reply: 4)
18. DEAE PREPARATION - (reply: 1)
19. His-TAG purification - (reply: 1)
20. Problem with GST tagged baculovirus protein - (reply: 1)
21. sandwich Ni column - (reply: 1)
22. protein solubility - (reply: 2)
23. native purification of His-tagged proteins from E. coli? - (reply: 1)
24. GST tagged protein purification - (reply: 8)
25. bacterial growth arrest after IPTG induction - (reply: 2)
26. Protein insolubility problem after bacterial induction - (reply: 2)
27. what cells to use for expression of pGEX3? - (reply: 1)
28. Analysis protein expression - (reply: 1)
29. Protein elution problems - (reply: 2)
30. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)