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Protease Related Discussions
  1. Store protease K, glycogen, DTT, RNaseOut at -80C? - Can I store these reagents at -80C instead of -20C ? (reply: 2)
  2. PMSF precipitate? - (reply: 5)
  3. protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
  4. Protease activity assay using pNA-peptide as substrates - Can anyone help me? (reply: 2)
  5. protease inhibitor, TRI reagent and western - (reply: 2)
  6. When to use PMSF in a cell lysis buffer? - (reply: 9)
  7. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
  8. PMSF? - (reply: 9)
  9. protease inhibitors screening methods - (reply: 1)
  10. what protease is best for primary cell culture - (reply: 1)
  11. Protease Inhibitors? - I want to know what are the best protease inhibitors to yeast. (reply: 3)
  12. Flag-IP elution with TEV protease - (reply: 1)
  13. PROTEASE ENHANCER - (reply: 1)
  14. Use of protease inhibitors when the target protein could be a protease that inte - (reply: 2)
  15. protease inhibitors in HPLC - trouble with PMSF at 215 nm (reply: 1)
  16. what would happen if i dilute protease inhibitor too much in cell lysate? - (reply: 3)
  17. HIV Protease Assay - inhibitor selection (reply: 1)
  18. ChIP Fixation & Protease inhibitors - (reply: 1)
  19. pmsf not dissolving in buffer - (reply: 3)
  20. Proteases - Tissue Preparation w/o Protease inhibitors (reply: 2)
  21. a compatible protease and phophatase inhibitors mix - (reply: 2)
  22. Protease / Phosphatase Inbibitor necessary ? - (reply: 4)
  23. Proteases and thier site cleavage - Enzyme digestion (reply: 2)
  24. Storage of B glycerophosphate NaF , Na3VO4, Na4P2O7 stock solution for lysis buf - How to store the protease kinase inhibitor soluble stock for lysis buf (reply: 3)
  25. His-tag: C-terminal cleavable through protease? - (reply: 1)
  26. how to dissolve PMSF - problem in dissolving PMSF (reply: 4)
  27. lysis buffer- protease inhibitors - (reply: 1)
  28. bacterial proteases? - (reply: 4)
  29. Protease Cocktail Inhibitor Complete from Roche? - (reply: 4)
  30. PMSF? - (reply: 5)
  31. serine protease - (reply: 1)
  32. Protease inhibitors - (reply: 8)
  33. Protease inhibitors used in CHIP - (reply: 2)
  34. Protease cutting at KK (lysine-lysine) points - know of any..?? (reply: 1)
  35. bacterial protease expression - expression (reply: 5)
  36. SUMO fusion proteins - problems with SUMO Protease (reply: 1)
  37. which protease is good - (reply: 9)
  38. substitution for PMSF? - (reply: 6)
  39. protease bands on the gel.... - should they be visible? (reply: 11)
  40. PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
  41. PMSF in AChE purification :unsure: - (reply: 1)
  42. can any one suggest list of protease inhibitors list & if possible reference - (reply: 2)
  43. matrix metallo proteases - (reply: 3)
  44. PMSF or liquid nitrogen - (reply: 1)
  45. Protease Inhibitors - (reply: 8)
  46. Protease purification - plant leaf protease (reply: 1)
  47. Protease: Post Electrophoretic Reactivation - (reply: 3)
  48. Is EDTA a protease inhibitor - a potentially stupid question (reply: 2)
  49. KB8301 MMP protease inhibitor - (reply: 8)
  50. Protease K preparation without Ca2+, Whether the Protease K acitivity is affecte - help me! (reply: 2)
  51. Phenol/Chloroform Extraction and Protease - (reply: 1)
  52. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
  53. shelf life of PMSF/ leupeptin in buffer - (reply: 1)
  54. sodium azide as protease inhibitor - protein overexpression (reply: 4)
  55. lysis buffer----SDS or PMSF? - (reply: 1)
  56. Protease in expression vector - (reply: 3)
  57. Expression cloning system for protease expression - (reply: 2)
  58. Protease inhibitor--Aprotinin activity - Ask for help! (reply: 1)
  59. Protease inhibitors - (reply: 1)
  60. protease inhibition - how to inhibit proteases? (reply: 1)
  61. PMSF - (reply: 8)
  62. Getting rid of PMSF - (reply: 3)