ChIP Related Discussions
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Crazy ChIP! Background probs and All the sudden no signal! - (reply: 2)
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monoclonal antibody or polyclonal antibody? - which is better for ChIP? (reply: 1)
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Question about sonication step for ChIP assay - (reply: 5)
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CHIP : Use frozen tissue - (reply: 1)
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Problem with qPCR of ChIP input - (reply: 11)
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ChIP sonication:different smear in cross-linked and non-corsslinked samples - (reply: 8)
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ChIP: SDS precipitates during sonication...any ideas? - (reply: 7)
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i was confused about ChIP result - i need help (reply: 1)
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Labelling ChIP samples - ARG! Hope someone can help! (reply: 1)
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ChIP and qPCR - ChIP and qPCR (reply: 4)
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Input problem with fast chip protocol - (reply: 6)
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ChIP densitometry - (reply: 1)
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control for ChIP - (reply: 2)
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input problem from ChIP prep - (reply: 5)
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Worries about ChIP results.. - (reply: 1)
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some questions about fast ChIP - (reply: 2)
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High quality formaldehyde for ChIP? - (reply: 2)
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Nuclear extraction of brain tissue for ChIP - (reply: 3)
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No DNA after sonication for ChIP - (reply: 2)
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ChIP sample storage - (reply: 4)
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ChIP Fixation & Protease inhibitors - (reply: 1)
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ChIp on mouse liver - (reply: 9)
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ChIP and qRT-PCR - (reply: 1)
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Fast ChIP Method and Chelex - (reply: 5)
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ChIP Troubleshoot - (reply: 1)
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Chip Assay - PCR step (reply: 3)
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ChIP qPCR data analysis? - (reply: 9)
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Magna ChIP kit from Upstate - Insufficient volume or reagents? (reply: 1)
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chip assay primers - chip assay primers (reply: 2)
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CHIP enrichment varies? anyone can help? - (reply: 3)
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ChIP on chip - can anyone help me? - (reply: 4)
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Magnetic ChIP - Magnetic Rack - (reply: 4)
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ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
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ChIP - Is it possible to spin beads too hard? - (reply: 8)
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How do you determine the linear range of ChIP and input samples? - (reply: 2)
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ChIP sonication problem - (reply: 7)
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Affymetrix chip - unexpressed genes, which signal cuttof to use - (reply: 1)
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no-antibody control is stronger than my sample! - in ChIP.. (reply: 5)
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Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
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ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
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RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
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ChIP on peripheral blood samples.... - anyone done it?? (reply: 2)
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ChIP: bright 300bp band after RNAse A - (reply: 1)
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Help: RNaseA or Proteinase K or both for DNA extraction in CHIP - (reply: 3)
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ChIP PCR problems - I can't get rid of the smears! - (reply: 6)
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CHIP calculation. confused! - (reply: 2)
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QPCR versus regular PCR for Chip - (reply: 2)
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ChIP has eaten all my DNA! - (reply: 1)
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good positive controls for Chip - (reply: 3)
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why do I see enrichment in ChIP inner control? - (reply: 2)
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How to obtain pure DNA from ChIP - (reply: 3)
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ChIP assay using Matrigel-3D cell culture - (reply: 2)
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positive IgG in ChIP - (reply: 2)
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ChIP - reproducibility problem - (reply: 9)
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what is meaning of true enrichment for chip qPCR? - (reply: 1)
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What in ChIP samples inhibit PCR? - (reply: 2)
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chip assay dna pptn - (reply: 3)
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ChIP'ing for Big, Indirect Coactivators - Looking for tips on how to get this difficult ChIP to work.. (reply: 6)
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Designation of PCR primers in ChIP experiment - (reply: 3)
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CHIP western - Western after CHIP (reply: 4)
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ChIP chip stripping - (reply: 1)
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PCR smear with CHIP - (reply: 2)
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Smear in PCR after ChIP - (reply: 2)
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how to purify ChIP DNA by using kit - (reply: 4)
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How many cycles in PCR would you recommend for ChIP - (reply: 3)
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ChIP with unknown DNA target - (reply: 2)
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ChIP and qPCR calculation ? - (reply: 8)
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Yeast ChIP Help - No DNA after sonication - - (reply: 2)
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Is this sonication result good for ChIP? - (reply: 6)
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ChIP PCR help, maybe is the problem of SDS. - (reply: 4)
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chromatin yield (ChIP) - chromatin yield from primary cells (reply: 3)
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Working antibody for human NFkB (p65) ChIP assay ? - (reply: 2)
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Input Control for ChIP - (reply: 3)
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what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
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Filamentous pellet when you sonicate in ChIP assay - (reply: 2)
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solid support for ChIP washes - (reply: 7)
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ChIP tag, Flag or HA sequence - (reply: 3)
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Companies for ChIP Ab - (reply: 2)
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ChIP IgG has the same signal than the antibody tested - (reply: 9)
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ChIP on chip Affymetrix - Ampification protocol - Quality of amplified DNA (reply: 5)
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Controls in ChIP experiments? - (reply: 5)
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ChIP DNA concentration - (reply: 7)
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CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
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Negative control primer for ChIP - (reply: 3)
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Using Westerns to check crosslinking in ChIP - (reply: 2)
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ChIP _ genomic DNA isolation - (reply: 1)
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Post ChIP DNA amount - (reply: 1)
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My primers don't work with Chip - (reply: 1)
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how to validated CHIP after H3k27 3Me - (reply: 5)
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ChIP in mouse tissues, How to crosslink ? - (reply: 1)
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CHIP: No enrichment in IP DNA Relative to Input - (reply: 4)
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ChIP assay! - (reply: 1)
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Can restriction digestion be replaced by CHIP? - (reply: 5)
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Special Question to KPDE - Fast chIP (reply: 2)
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Protease inhibitors used in CHIP - (reply: 2)
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what is the perfect volume for immunoprecipitation step of ChIP? - (reply: 4)
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real time PCR with ChIP - (reply: 4)
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How do people shear their chromatin for ChIP? - Tips and Hints with Fragmenting Chromatin (reply: 1)
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CHIP sonication chromatin size - (reply: 1)
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ChIP: I get smear after PCR? - (reply: 2)
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ChIP on Affy's Human Promoter 1.0R array - (reply: 20)
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Why my ChIP PCR results are always unrepeatable? even from the same sample. - (reply: 7)
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Titrate chromatin for chIP - (reply: 2)
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Help with ChIP analysis using Taqman - (reply: 1)
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ChIP DNA yield problem - (reply: 2)
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No product after chIP? - (reply: 1)
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Selection of Antibodies for CHip - (reply: 7)
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Please Help me, about Chromatin IP(ChIP)! - CHIP (reply: 1)
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problem of DNA sonication in CHIP assay - (reply: 2)
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Please check my ChIP sonication gel - (reply: 4)
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Does anyone know any kind of array or chip for methylation scan? - (reply: 3)
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About two-step Chip - (reply: 1)
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testing CHIP samples on Western - how to de-crosslink (reply: 2)
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ChIP assay results interpretations - (reply: 3)
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Is ChIP is a good choice to find the DNA sequence for a binding protein? - (reply: 2)
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ChIP and CpG island microarrays - (reply: 1)
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Dynal (Magnetic) beads for ChIP? - (reply: 3)
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ChIP - (reply: 1)
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ChIP sonication problem - (reply: 4)
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How to purify the ChIP DNA - (reply: 4)
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ChIP assay - (reply: 2)
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problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
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My ChIP product Western is dirty - (reply: 7)
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Has anyone read about doing ChIP on tumor specimans? - (reply: 1)
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why couldn't I see my protein on western blot after ChIP? - (reply: 3)
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The Histone H3.1 and Histone H3.3 variant quandry - How can you tell the difference in a ChIP?? (reply: 2)
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ChIP Assay and no clear answer from professor - (reply: 3)
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ChIP on frozen tissue samples - (reply: 7)
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ChIP: Equal DNA Pull-Down in Positive and Negative Controls - HELP - (reply: 5)
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ChIP from low amount of cells - (reply: 1)
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Problem in first step in ChIP - (reply: 9)
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ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
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ChIP control for transcriptional repressor? - (reply: 1)
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How long can the whole cell extract for ChIP stay in -80C? - (reply: 1)
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EMSA done...what would ChIP assay add - (reply: 2)
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how to evaluate chIP enrichment efficency? - (reply: 1)
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ChIP crosslinking - (reply: 2)
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ChIP qPCR analysis - (reply: 2)
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Got different size PCR products from chIP - (reply: 7)
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How can I extract Chromatin-protein complex without using Chip? - The cheapest method to prove a protein to bind DNA (reply: 3)
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ChIP sonication problems...can I use 20 sec pulse? - (reply: 2)
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ChIP control problems - (reply: 1)
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how everyone obtain skills for chip assay? - Is it necessary to learn it from another lab? (reply: 5)
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positive controls for CHIP - (reply: 2)
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pre-immune serum in CHIP IP - (reply: 1)
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ChIP sonication problems - shearing dna before actually sonicating (reply: 3)
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How to design PCR primers in ChIP assay - (reply: 2)
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strange CHIP input result - (reply: 3)
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How to test ChIP cross-linking and reversal? - (reply: 18)
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ChIP sonication problem: too wide range of smear - too wide range of smear (reply: 9)
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how many antibody eveyone use for CHIP assay - (reply: 2)
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CHIP and CHIP with tagged proteins - (reply: 1)
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Advantages of ChIP over EMSA - (reply: 3)
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internal assay controls for histone ChIP - (reply: 6)
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ChIP protocol and tips - (reply: 8)
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function of LiCl in ChIP wash buffer - (reply: 1)
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CHIP - Harvesting cells by trypsin - (reply: 1)
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starting amount of DNA for ChIPs - using the ChIP for ChIP-on-chip analysis (reply: 1)
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HELP! Background in ChIP assay - (reply: 1)
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ChIP Normalization - (reply: 2)
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DNA shearing optimization for ChIP assay - (reply: 2)
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CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
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Cross-linking and cross-link reversal for ChIP - (reply: 3)
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ChIP quantification (qPCR) - (reply: 2)
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ChIP DNA purification - URGENT please (reply: 8)
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Nonspecific precipitation in ChIP - (reply: 4)
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ChIP assay sonication problem - (reply: 33)
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6 Hour ChIP method - (reply: 3)
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ChIP analysis using tissue and controls - (reply: 3)
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ChIP - no immunoprecipitated DNA - (reply: 12)
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ChIP: Sonication - (reply: 7)
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ChIP protokol question - (reply: 2)
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DNA amount in ChIP - (reply: 2)
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Reliable ChIP primers for Sp1? - (reply: 2)
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CHIP on Chip array - (reply: 11)
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Has anyone tried to sonicate Jurkat cells for ChIP assay? - (reply: 7)
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ChIP sonication Input Agarose gel - (reply: 1)
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CHIP with Tagged Proteins - (reply: 2)
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help with ChIP control--urgent! - (reply: 6)
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Nuclear extracts for ChIP? - (reply: 1)
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Avoiding non-specific DNA binding in ChIP assays - (reply: 3)
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ChIP DNA sonication conditions - (reply: 3)
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Problematic results with ChIP - need help troubleshooting - (reply: 3)
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ChIp using Drossophila imaginal discs - (reply: 1)
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qPCR after ChIP - lack of reproducibility - (reply: 9)
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CHIP FOR CBP - (reply: 1)
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Blocking in ChIP - (reply: 2)
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MNase vs sonication for ChIP - (reply: 6)
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On antibody storage and ChIP - (reply: 3)
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affymetrix gene chip compering to agilent gene chip - (reply: 2)
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ChIP sonication using a cup horn - (reply: 1)
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Variability in ChIP assay - (reply: 4)
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Trascruz reagents for ChIP assays - (reply: 3)
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DNA length to be amplified in ChIP. - What should be DNA size range? (reply: 1)
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ChIP analysis - how to analyze CHIP data accuretly? - (reply: 2)
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Discussion: ChIP PCR control - (reply: 12)
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No PCR products with the ChIP DNA - (reply: 5)
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SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
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lightcycler controls and standard for CHIP - (reply: 3)
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Which antibody to use for ChiP? - Monoclonal or polyclonal? (reply: 5)
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Problems in CHIP assay? - How can i get rid of multiple bands in CHIP assay? (reply: 3)
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amount of DNA after Chip - (reply: 1)
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Can ChIP be applied on tissue samples? - (reply: 5)
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ChIP Negative Control - Chromatin Immunoprecipitation (reply: 1)
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ChIP lysis buffer - Chromatin (reply: 2)
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Using qiagen kit for ChIP DNA purification - (reply: 4)
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Sepharose or agarose in ChIP assay - (reply: 2)
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ChIP with primary human CD4+ Lymphocytes possible ? - (reply: 1)
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Q re: optimizing sonication in ChIP - (reply: 2)
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CHIP - region - (reply: 1)
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ChIP again... - (reply: 1)
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tissue ChIP - (reply: 2)
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Can I use the upstate ChIP protocol for mouce tissue? - (reply: 5)
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Background in CHIP - (reply: 7)
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USING MISONIX MICROSON FOR SONICATION FOR CHIP ANALYSIS - (reply: 1)
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ChIP sample storage - (reply: 5)
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Question about ChIP? - (reply: 2)
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ChIP - (reply: 9)
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CHIP with nuclei - (reply: 1)
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ChIP assay loading control - (reply: 6)
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ChIP DNA sonication problem - got too big and too small fragments (reply: 3)
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non-specific band in ChIP assay - (reply: 1)
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ChIP assay - Foaming in sonication and other woes - (reply: 41)
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Ampification in no-antibody control of ChIP assay - High background - (reply: 13)
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ChIP against transcription factors - (reply: 7)
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PCR for ChIP - PCR for ChIP not working (reply: 3)
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ChIP sonication woes - Losing DNA? (reply: 4)
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sonication for ChIP - protocols for sonication in a water bath for ChIP (reply: 2)
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ChIP Assay and sonicators - ChIP assay question (reply: 4)
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FLAG (M2) EZView beads in ChIP assay - Background binding trouble (reply: 4)
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ChIP assay - (reply: 2)
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CHIP assay - (reply: 1)