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GFP Related Discussions
  1. GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
  2. GFP Tobacco BY-2 cell sorting - (reply: 1)
  3. Detection of GFP expressing cells - fix or not? - (reply: 3)
  4. observation of GFP fusion by LSCM in root tips - observation of GFP fusion by LSCM in root tips (reply: 4)
  5. N terminal GFP fusions - is it as appropriate as C terminus? (reply: 3)
  6. lentivirus transduction - no GFP after trasduction (reply: 5)
  7. quenching of GFP after selection of stable cells - (reply: 1)
  8. stable cell line, but quenched GFP? - (reply: 2)
  9. is my GFP quenched? - (reply: 2)
  10. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  11. Will GFP interferes with luciferase assay result? - (reply: 3)
  12. HEK cell, EGFP and PI staning - (reply: 7)
  13. GFP western - (reply: 3)
  14. Can I use DAPI and GFP at the same time? - Need advice on fluorochromes for confocal microscopy (reply: 8)
  15. Imaging GFP tagged proteins, background signal due to contamination? - Imaging, background spots/speckles, contamination (reply: 6)
  16. EGFP stable cell line proof - (reply: 3)
  17. Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
  18. Living cell GFP imaging - (reply: 1)
  19. Living cell GFP imaging - (reply: 6)
  20. Replacing EGFP with RFP..but no expression! - (reply: 10)
  21. EGFP constructed in T-vector can express green fluorescence? - SOS (reply: 6)
  22. question on colocalization of mitochondrion and a GFP fusion - (reply: 2)
  23. colocalization of mitochondrion and GFP fusion - (reply: 2)
  24. after PFA fixation, EGFP intensity fated away over time - Anyone noticed this? (reply: 9)
  25. GFP, both in the nucleus and in the cytoplasm - transfection (reply: 8)
  26. no GFP in cells - (reply: 7)
  27. GFP imaging - (reply: 2)
  28. How can I transform GFP into recA -/- E. coli? - (reply: 1)
  29. brdu staining on gfp+ve cells - (reply: 1)
  30. BrDU staining on GFP positive cells - (reply: 1)
  31. No expression from injected GFP&RFP mRNA - (reply: 16)
  32. Stable transfection with or without EGFP - (reply: 3)
  33. How to get GFP producing bacteria? - help? (reply: 5)
  34. How to quantify GFP fluorescence from a bacteria? - (reply: 3)
  35. GFP/RFP/YFP G418 resistant plasmids - Help please (reply: 1)
  36. Destroying GFP fluorescence - (reply: 5)
  37. Reduction in EGFP after sorting - (reply: 4)
  38. Has anybody added artificial introns into GFP coding sequence. - (reply: 1)
  39. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
  40. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
  41. Problem with gfp express in S2 - (reply: 7)
  42. Detection of GFP in supernatant - (reply: 2)
  43. Sindbis virus expression - cannot detect EGFP signal in hippocampal neurons (reply: 1)
  44. gus or gfp - gus or gfp (reply: 2)
  45. FACS GFP leakage - (reply: 4)
  46. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
  47. EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
  48. Loss of GFP - (reply: 2)
  49. using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
  50. HELP! GFP plasmids - GFP plasmids (reply: 4)
  51. half life of EGFP protein - (reply: 3)
  52. GFP transfection - basic protocol needed (reply: 1)
  53. loss of GFP upon selection - (reply: 4)
  54. a question about screening transfected cells by GFP protein - (reply: 5)
  55. GFP GOLGI - Expresion of pAcGFP Golgi. (reply: 3)
  56. gfp fusion proteins - is liker needed if so how many amino acids usual choice? (reply: 2)
  57. Help! GFP transfection - (reply: 12)
  58. GFP siRNA transfection - readout - (reply: 5)
  59. GFP Fusion protein - (reply: 6)
  60. GFP to expression - (reply: 3)
  61. why does empty EGFP vector has no fluorescence? :/ - (reply: 5)
  62. why EGFP become weak in C2C12? - (reply: 3)
  63. Expression of GFP fusion protein - Improving fluorescence visualization (reply: 2)
  64. "Phantom" GFP bands - (reply: 2)
  65. Help! GFP is bleached after immunostaining. - (reply: 2)
  66. immunostaining but GFP is bleached - (reply: 1)
  67. Problem with EGFP in translation study - (reply: 12)
  68. GFP labelled protein purification - (reply: 1)
  69. GFP visualisation in GloFish - (reply: 1)
  70. Detection on plate for a bacteria who express GFP? - (reply: 7)
  71. gfp digested with Ecor1 + Hind III, and Bgl II - (reply: 4)
  72. GFP and DsRed dual lenti construct - (reply: 4)
  73. BrdU incorporation and GFP staining - (reply: 3)
  74. EGFP and GFP - how different are they? (reply: 8)
  75. Fixation for eGFP imaging? - what fixation should I use? (reply: 3)
  76. GFP expression - microscopy, spectrofluorimetry (reply: 3)
  77. anti GFP - IP - (reply: 3)
  78. GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
  79. can i still see the GFP reaction after the distruction of cell. - (reply: 1)
  80. Protocol:GFP reporter assay - (reply: 4)
  81. Stop codon before GFP - Stop codon before GFP (reply: 7)
  82. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  83. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
  84. picked GFP transfected colonies do not retain flouresence after being picked - (reply: 3)
  85. dsRNA of EGFP as control? - (reply: 4)
  86. transfection gfp visualization - (reply: 14)
  87. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
  88. GFP as a reporter gene? - (reply: 5)
  89. Transfection efficiency by GFP - (reply: 4)
  90. Cell cycle analysis (FACS) analysis and GFP detection - (reply: 10)
  91. Join GFP to my insert without mutagenesis - (reply: 8)
  92. GFP or LacZ for quantification of promoter activity? - (reply: 4)
  93. detection of GFP fluorescence in whole cell bacteria - (reply: 1)
  94. Expression of N-terminal GFP fusionproteins - Proteins with N-terminal signalpeptide (reply: 3)
  95. Localization of GFP - (reply: 2)
  96. Half life of EGFP - (reply: 1)
  97. Site directed mutagenesis GFP to RFP - (reply: 4)
  98. destabilized EGFP Vector urgently needed - (reply: 4)
  99. siRNA /shRNA agains EGFP - (reply: 4)
  100. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
  101. EGFP detection - (reply: 4)
  102. GFP protein not soluble? - (reply: 1)
  103. pGen.IRES.GFP.Neo - (reply: 2)
  104. FACS: DNA content and GFP in one go!!! - PI masks GFP??? (reply: 4)
  105. Problems with co-localization (GFP+lysotracker red) - (reply: 2)
  106. How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
  107. Cloning into GFP vector - (reply: 2)
  108. invisible GFP - (reply: 2)
  109. cloning problem - cloning problem in GFP vector (reply: 4)
  110. western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
  111. Ligation into EGFP problems - Ligation into EGFP problems (reply: 5)
  112. GFP - (reply: 1)
  113. DNase,BrdU and GFP - (reply: 1)
  114. GFP transfection using lipofectin - (reply: 1)
  115. GFP ELISA essay - (reply: 1)
  116. Green Fluroscent Protein - Identification and Characterization of Promoter usin GFP (reply: 1)