Protocol Online logo
Top : Forum Archives: : Indexed by Topic
GFP Related Discussions
  1. EGFP cloning and membrane protein - (reply: 2)
  2. egfp in ecoli (pet60) - (reply: 1)
  3. Does FRET by BFP/GFP work? - (reply: 2)
  4. GFP can not be observed! Help. - (reply: 6)
  5. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
  6. High GFP background on FACS - (reply: 3)
  7. SOS help for GFP leakage during FACS - (reply: 2)
  8. Need help with transfection! - GFP + HSP (reply: 3)
  9. Question in EGFP saw fluorescence but read zero in fluoremeter - (reply: 1)
  10. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
  11. What is the most sensitive assay for promoter strength? - dual luciferase assay? GFP? SEAP assay? Miller or something else? (reply: 3)
  12. GFP intercellular Localisation ; Helpneeded - (reply: 2)
  13. Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (reply: 2)
  14. cutting out EGFP gene from pEGFP-N1 vector - what does dam- mean? (reply: 1)
  15. Expression of GFP and DsRed tagged proteins - co-localization needed for FRET (reply: 9)
  16. half life time of GFP? - (reply: 1)
  17. help~ GFP fluorescence - (reply: 1)
  18. GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
  19. GFP Tobacco BY-2 cell sorting - (reply: 1)
  20. Detection of GFP expressing cells - fix or not? - (reply: 3)
  21. observation of GFP fusion by LSCM in root tips - observation of GFP fusion by LSCM in root tips (reply: 4)
  22. N terminal GFP fusions - is it as appropriate as C terminus? (reply: 3)
  23. lentivirus transduction - no GFP after trasduction (reply: 5)
  24. quenching of GFP after selection of stable cells - (reply: 1)
  25. stable cell line, but quenched GFP? - (reply: 5)
  26. is my GFP quenched? - (reply: 2)
  27. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  28. Will GFP interferes with luciferase assay result? - (reply: 3)
  29. HEK cell, EGFP and PI staning - (reply: 7)
  30. GFP western - (reply: 3)
  31. Can I use DAPI and GFP at the same time? - Need advice on fluorochromes for confocal microscopy (reply: 8)
  32. Imaging GFP tagged proteins, background signal due to contamination? - Imaging, background spots/speckles, contamination (reply: 6)
  33. EGFP stable cell line proof - (reply: 3)
  34. Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
  35. Living cell GFP imaging - (reply: 1)
  36. Living cell GFP imaging - (reply: 6)
  37. Replacing EGFP with RFP..but no expression! - (reply: 10)
  38. EGFP constructed in T-vector can express green fluorescence? - SOS (reply: 6)
  39. question on colocalization of mitochondrion and a GFP fusion - (reply: 2)
  40. colocalization of mitochondrion and GFP fusion - (reply: 2)
  41. after PFA fixation, EGFP intensity fated away over time - Anyone noticed this? (reply: 9)
  42. GFP, both in the nucleus and in the cytoplasm - transfection (reply: 8)
  43. no GFP in cells - (reply: 7)
  44. GFP imaging - (reply: 2)
  45. How can I transform GFP into recA -/- E. coli? - (reply: 1)
  46. brdu staining on gfp+ve cells - (reply: 1)
  47. BrDU staining on GFP positive cells - (reply: 1)
  48. No expression from injected GFP&RFP mRNA - (reply: 16)
  49. Stable transfection with or without EGFP - (reply: 3)
  50. How to get GFP producing bacteria? - help? (reply: 5)
  51. How to quantify GFP fluorescence from a bacteria? - (reply: 3)
  52. GFP/RFP/YFP G418 resistant plasmids - Help please (reply: 1)
  53. Destroying GFP fluorescence - (reply: 5)
  54. Reduction in EGFP after sorting - (reply: 4)
  55. Has anybody added artificial introns into GFP coding sequence. - (reply: 1)
  56. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
  57. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
  58. Problem with gfp express in S2 - (reply: 7)
  59. Detection of GFP in supernatant - (reply: 2)
  60. Sindbis virus expression - cannot detect EGFP signal in hippocampal neurons (reply: 1)
  61. gus or gfp - gus or gfp (reply: 2)
  62. FACS GFP leakage - (reply: 4)
  63. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
  64. EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
  65. Loss of GFP - (reply: 2)
  66. using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
  67. HELP! GFP plasmids - GFP plasmids (reply: 4)
  68. half life of EGFP protein - (reply: 3)
  69. GFP transfection - basic protocol needed (reply: 1)
  70. loss of GFP upon selection - (reply: 4)
  71. a question about screening transfected cells by GFP protein - (reply: 5)
  72. GFP GOLGI - Expresion of pAcGFP Golgi. (reply: 3)
  73. gfp fusion proteins - is liker needed if so how many amino acids usual choice? (reply: 2)
  74. Help! GFP transfection - (reply: 12)
  75. GFP siRNA transfection - readout - (reply: 5)
  76. GFP Fusion protein - (reply: 6)
  77. GFP to expression - (reply: 3)
  78. why does empty EGFP vector has no fluorescence? :/ - (reply: 5)
  79. why EGFP become weak in C2C12? - (reply: 3)
  80. Expression of GFP fusion protein - Improving fluorescence visualization (reply: 2)
  81. "Phantom" GFP bands - (reply: 2)
  82. Help! GFP is bleached after immunostaining. - (reply: 2)
  83. immunostaining but GFP is bleached - (reply: 1)
  84. Problem with EGFP in translation study - (reply: 12)
  85. GFP labelled protein purification - (reply: 1)
  86. GFP visualisation in GloFish - (reply: 1)
  87. Detection on plate for a bacteria who express GFP? - (reply: 7)
  88. gfp digested with Ecor1 + Hind III, and Bgl II - (reply: 4)
  89. GFP and DsRed dual lenti construct - (reply: 4)
  90. BrdU incorporation and GFP staining - (reply: 3)
  91. EGFP and GFP - how different are they? (reply: 8)
  92. Fixation for eGFP imaging? - what fixation should I use? (reply: 3)
  93. GFP expression - microscopy, spectrofluorimetry (reply: 3)
  94. anti GFP - IP - (reply: 3)
  95. GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
  96. can i still see the GFP reaction after the distruction of cell. - (reply: 1)
  97. Protocol:GFP reporter assay - (reply: 4)
  98. Stop codon before GFP - Stop codon before GFP (reply: 7)
  99. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  100. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
  101. picked GFP transfected colonies do not retain flouresence after being picked - (reply: 3)
  102. dsRNA of EGFP as control? - (reply: 4)
  103. transfection gfp visualization - (reply: 14)
  104. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
  105. GFP as a reporter gene? - (reply: 5)
  106. Transfection efficiency by GFP - (reply: 4)
  107. Cell cycle analysis (FACS) analysis and GFP detection - (reply: 10)
  108. Join GFP to my insert without mutagenesis - (reply: 8)
  109. GFP or LacZ for quantification of promoter activity? - (reply: 4)
  110. detection of GFP fluorescence in whole cell bacteria - (reply: 1)
  111. Expression of N-terminal GFP fusionproteins - Proteins with N-terminal signalpeptide (reply: 3)
  112. Localization of GFP - (reply: 2)
  113. Half life of EGFP - (reply: 1)
  114. Site directed mutagenesis GFP to RFP - (reply: 4)
  115. destabilized EGFP Vector urgently needed - (reply: 4)
  116. siRNA /shRNA agains EGFP - (reply: 4)
  117. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
  118. EGFP detection - (reply: 4)
  119. GFP protein not soluble? - (reply: 1)
  120. pGen.IRES.GFP.Neo - (reply: 3)
  121. FACS: DNA content and GFP in one go!!! - PI masks GFP??? (reply: 4)
  122. Problems with co-localization (GFP+lysotracker red) - (reply: 2)
  123. How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
  124. Cloning into GFP vector - (reply: 2)
  125. invisible GFP - (reply: 2)
  126. cloning problem - cloning problem in GFP vector (reply: 4)
  127. western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
  128. Ligation into EGFP problems - Ligation into EGFP problems (reply: 5)
  129. GFP - (reply: 1)
  130. DNase,BrdU and GFP - (reply: 1)
  131. GFP transfection using lipofectin - (reply: 1)
  132. GFP ELISA essay - (reply: 1)
  133. Green Fluroscent Protein - Identification and Characterization of Promoter usin GFP (reply: 1)