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1. taq Gold and rox buffer - (reply: 2)
2. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
3. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 4)
4. SYBR green - (reply: 1)
5. Two peaks in melt curve when I increase master mix volume - (reply: 3)
6. contamination issue in -RT control - (reply: 1)
7. QPCR - (reply: 9)
8. QPCR - (reply: 2)
9. Syber green - (reply: 4)
10. Standard curve from plasmid - for cDNA quantification (reply: 6)
11. High baseline/noise probes - (reply: 2)
12. problems cleaning up small DNA products - (reply: 2)
13. interpretation of results of relative qRTPCR - please help (reply: 5)
14. New to qPCR - (reply: 2)
15. Sybr Green real time pcr limit of detection - (reply: 1)
16. real-time PCR problem - (reply: 1)
17. Can i use old standard curve to determine gene concentration? - (reply: 1)
18. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
19. Creating standard curve - (reply: 1)
20. Real-time PCR for mRNA stability - (reply: 2)
21. Cre genotyping - smeared results (reply: 1)
22. Standard Curve method RT PCR- - (reply: 2)
23. Troubleshooting in quantifying Genomic/WGA DNA - (reply: 5)
24. Large smear in PCR - (reply: 2)
25. kras mutation. best method for detection - (reply: 3)
26. developing real time PCR - (reply: 5)
27. First time Real TIme - (reply: 2)
28. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
29. geNorm and normalising factor - (reply: 3)
30. Help designing primers; also help with viewing GenBank - (reply: 3)
2. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
3. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 4)
4. SYBR green - (reply: 1)
5. Two peaks in melt curve when I increase master mix volume - (reply: 3)
6. contamination issue in -RT control - (reply: 1)
7. QPCR - (reply: 9)
8. QPCR - (reply: 2)
9. Syber green - (reply: 4)
10. Standard curve from plasmid - for cDNA quantification (reply: 6)
11. High baseline/noise probes - (reply: 2)
12. problems cleaning up small DNA products - (reply: 2)
13. interpretation of results of relative qRTPCR - please help (reply: 5)
14. New to qPCR - (reply: 2)
15. Sybr Green real time pcr limit of detection - (reply: 1)
16. real-time PCR problem - (reply: 1)
17. Can i use old standard curve to determine gene concentration? - (reply: 1)
18. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
19. Creating standard curve - (reply: 1)
20. Real-time PCR for mRNA stability - (reply: 2)
21. Cre genotyping - smeared results (reply: 1)
22. Standard Curve method RT PCR- - (reply: 2)
23. Troubleshooting in quantifying Genomic/WGA DNA - (reply: 5)
24. Large smear in PCR - (reply: 2)
25. kras mutation. best method for detection - (reply: 3)
26. developing real time PCR - (reply: 5)
27. First time Real TIme - (reply: 2)
28. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
29. geNorm and normalising factor - (reply: 3)
30. Help designing primers; also help with viewing GenBank - (reply: 3)