1. qRT-PCR: how to average multiple runs? - (reply: 1)
2. Definition - (reply: 1)
3. Transcript variation - measuring by RT-PCR - (reply: 3)
4. Primer analysis sites - Any suggestions? (reply: 2)
5. Problem with promoter PCR and cloning - (reply: 3)
6. DNA blunting - DNA blunting troubleshoot (reply: 4)
7. Identification of weakly expressed novel gene - (reply: 1)
8. pIRES-EGFP - (reply: 2)
9. TA Cloning - TA cloning (reply: 2)
10. Large DNA fragmnet ampification - (reply: 6)
11. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
12. Long Range PCR - (reply: 2)
13. please help me to be sure from design primer have it - (reply: 3)
14. Methyltransferase specificity - (reply: 1)
15. reverse-transcriptase PCR (internal control?) - (reply: 2)
16. QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
17. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
18. RNA gel - too many bands?? (reply: 3)
19. Agrobacterium miniprep problem - (reply: 4)
20. Luciferase reporter assay - (reply: 1)
21. LR PCR and running gels - Roche Expand Long Range dNTPack (reply: 2)
22. Improved electrophoresis of nucleic acids. - (reply: 11)
23. separating protein and nucleic acids - problem separating protien and nucleic acids (reply: 8)
24. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
25. 16s RNA of metagenomic DNA - soil bacteria (reply: 2)
26. HIV-pCL-CG - problem w/ bacteria - (reply: 1)
27. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
28. Digest with BglII and SalI - (reply: 1)
29. DNA migration problem - Genomic DNA (reply: 1)
30. bacterial DNA isolation - various methods (reply: 1)

© 1999-2008 Protocol Online, All rights reserved.