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Ligation Related Discussions
  1. Ligation problem again - (reply: 4)
  2. BamHI/XhoI ligation - (reply: 3)
  3. ligation problems....? - (reply: 1)
  4. self ligation - (reply: 5)
  5. Ligation buffer - DTT in ligation buffer (reply: 1)
  6. Help, no insert, ligation product couldn't be cut anymore! - (reply: 3)
  7. my strategy of ligation - (reply: 5)
  8. Blunt end ligation problem - (reply: 3)
  9. directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
  10. Blunt end ligation problem - (reply: 2)
  11. Inverse PCR ligation - (reply: 1)
  12. ligation problem by using single RE - (reply: 8)
  13. blunt-end ligation - (reply: 2)
  14. T4 Ligase problem - Please help me, (reply: 10)
  15. ligation and transformation problems - (wrong) oligos (reply: 4)
  16. 3 piece ligation - (reply: 4)
  17. Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation (reply: 8)
  18. "ladder" ligation result - cloning is the bane of my existance (reply: 18)
  19. before electro-transformation, remove T4 Ligase or not? - (reply: 3)
  20. shRNA ligation problem - (reply: 3)
  21. pUC19 ligation - (reply: 1)
  22. Ligation problems... - (reply: 2)
  23. Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
  24. blunt ligation of large fragment - (reply: 6)
  25. ligase temperature - (reply: 5)
  26. transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
  27. My first ever 3-way ligation :) - Advice on ratios? (reply: 5)
  28. Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)
  29. question in ligation - (reply: 1)
  30. Ligation problem - 2 Questions (reply: 5)
  31. PEG 4000 for ligation - (reply: 5)
  32. 3 way Ligation Issues... - (reply: 11)
  33. It keeps getting better and better lol my ligase might be out to get me - (reply: 2)
  34. Insert/vector ratio for ligation - (reply: 3)
  35. Ligation using TA cloning - (reply: 1)
  36. blunt end ligation - (reply: 2)
  37. ligation failed - (reply: 1)
  38. TA cloning and 3 fragment ligation problems - (reply: 1)
  39. Ligation problem - (reply: 7)
  40. Ligation SalI / XhoI - (reply: 8)
  41. Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
  42. Ask about linker ligation - (reply: 6)
  43. how long can I keep a ligation reaction going at 4 C? - (reply: 3)
  44. methylation affects ligation? - (reply: 2)
  45. Sequencing incorrect after digestion and ligation - (reply: 5)
  46. Blunt end ligation - (reply: 8)
  47. DNA gel to Test Ligation? - (reply: 2)
  48. dilute the ligation reaction for electroporation - (reply: 7)
  49. Ligation failure - (reply: 1)
  50. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
  51. double digestion and ligation - (reply: 3)
  52. 3 piece ligation - (reply: 2)
  53. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
  54. Ligation: time and temperature - (reply: 8)
  55. ligation - very small insert - problems during ligation (reply: 11)
  56. Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
  57. Second-strand synthesis - why use ligase? - DNA polI - mediated SSS (reply: 1)
  58. About Ligation Independent Cloning - share the opinions (reply: 3)
  59. Ligation Reaction - (reply: 1)
  60. ligation problem - (reply: 4)
  61. Ligation problem? - (reply: 10)
  62. setting up an ligation reaction - (reply: 5)
  63. Problem with ligation..! - (reply: 5)
  64. questions about mung bean nuclease and ligation - (reply: 1)
  65. No ligation! Can't understand why! - (reply: 13)
  66. purification、ligation and transformation problems - (reply: 11)
  67. ligation failure-what to do? - tried everything but still not getting transformants (reply: 12)
  68. ligation transformation.......which chemically competent cells are the best? - (reply: 3)
  69. transfection with ligation mix? - (reply: 6)
  70. long insert ligation - (reply: 5)
  71. Big fragment ligation - (reply: 6)
  72. Small fragment sticky-end ligation into EGFP-N1 vector - (reply: 2)
  73. Do you discard phosphatase before ligation? - (reply: 6)
  74. T4 DNA ligase still usable after a night out? - (reply: 3)
  75. Ligation problem with PCR product cloning - (reply: 9)
  76. PCR cloning-primer design and ligation - (reply: 20)
  77. LIGATION DOESNT WORK - (reply: 7)
  78. Help! Suggestions for ligation ratios - (reply: 8)
  79. Ligation and Transformation Problem - (reply: 2)
  80. Klenow - ligation problem - Can Klenow add some extra nucleotides.. (reply: 7)
  81. Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
  82. Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
  83. Question about ligation! - Ligation of 6kb insert in pGEM T-vector (reply: 1)
  84. Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (reply: 2)
  85. Need help for 3 fragment ligation using pGEM T - (reply: 7)
  86. Which enzyme for repairing nicks in ligated constructs? - (reply: 1)
  87. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
  88. Reaction volume for ligation - (reply: 5)
  89. Great ligation problem - Double digested DNA insertion problem (reply: 5)
  90. Blunt ended ligation not working! - (reply: 3)
  91. Absolutely no Ligation product - (reply: 6)
  92. pros and cons of Blunt vs sticky ligation - (reply: 2)
  93. Blunt end ligation problems, no ligation at all... - (reply: 2)
  94. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  95. ligation problem - (reply: 3)
  96. Self Circularization Problem with a 6KB vector - self ligation of a 6KB vector (reply: 1)
  97. cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
  98. Ligation of small insert - (reply: 4)
  99. unable to clone into pET29b and pET32b - sticky end ligation failing (reply: 2)
  100. How long do you ligate? - (reply: 6)
  101. Restriction enzyme sites mutate after ligation? - (reply: 2)
  102. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  103. Ligation problems using NEB Quick Ligase Kit - (reply: 5)
  104. DNA disappeared after ligation - (reply: 2)
  105. Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
  106. Why Ligation and transformation not working? - (reply: 3)
  107. why the difference in ligation temperature? - (reply: 1)
  108. Ligation for two overnights. Is it bad? - (reply: 2)
  109. Ligate with 01 enzyme site - (reply: 5)
  110. Ligase always needed for DNA circularisation ? - (reply: 5)
  111. Transformation problem with newly ligated plasmids - (reply: 1)
  112. Product of ligation storage - (reply: 3)
  113. short oligos ligation and transformation - (reply: 3)
  114. multi fragment ligations - (reply: 2)
  115. T4 ligation problems with adapters - (reply: 1)
  116. getting faint band during ligation - (reply: 7)
  117. problem with ligation of big fragments - (reply: 3)
  118. ligation problem - (reply: 5)
  119. Unable to ligate two PCR products - (reply: 4)
  120. ligation - (reply: 5)
  121. how to ligate a gene repeatedly - (reply: 6)
  122. Is my DNA clean enough for a succesful ligation? - (reply: 15)
  123. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
  124. Plasmid Ligation does not work-suggestions? - (reply: 6)
  125. ligation transformation kit - fermentas kit (reply: 4)
  126. Circular ligation product migration on gel? - (reply: 1)
  127. Ligation and transformation problem - (reply: 4)
  128. concentration of vector in ligation reaction - (reply: 4)
  129. Why the ligation does not work? - (reply: 7)
  130. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
  131. three way ligation - three way ligation help (reply: 6)
  132. Ligation with T4 DNA Ligase - (reply: 9)
  133. 5' adaptor ligation fail - (reply: 3)
  134. How much ligase per ug of DNA? - (reply: 6)
  135. Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
  136. ligation step in cosmid library construction - (reply: 3)
  137. 3 molar insert excess - how imp. is it for the ligation rxn (reply: 2)
  138. Quick T4 DNA ligase - optimizing the condition for quick ligase (reply: 6)
  139. ligation problem - (reply: 11)
  140. Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
  141. T4 DNA ligase Buffer - (reply: 3)
  142. T4 DNA ligase - (reply: 1)
  143. Is time of ligation insert and vector crucial? - (reply: 4)
  144. Help with Ligation - (reply: 3)
  145. Ligation - (reply: 5)
  146. Problem with Ligation - (reply: 4)
  147. blunt ending the vector followed by the ligation - (reply: 1)
  148. Multiple fragment ligation - (reply: 7)
  149. HELP: Ligation Problems - (reply: 10)
  150. three-point ligation - (reply: 6)
  151. Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
  152. Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
  153. does the high pH of milli Q water will affect my ligation? - (reply: 8)
  154. T4 DNA ligase - (reply: 1)
  155. Double-stranded oligo ligation - (reply: 13)
  156. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
  157. problems in ligation/transformation - (reply: 12)
  158. ligation ratio - (reply: 1)
  159. Ligation with 3 inserts - (reply: 1)
  160. Can a single restricted vector spontaneously ligate back to a circular DNA? - (reply: 3)
  161. Quick ligation and blunting kits NEB - (reply: 7)
  162. Ligation Difficulty - (reply: 2)
  163. ligated vs. supercoiled - (reply: 8)
  164. Lots of colonies but they're all blue! - Do I need to adjust ligation ratios? (reply: 11)
  165. Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
  166. "Ligate" two overlapping pcr products with PCR fusion? - (reply: 1)
  167. Ligation/ transforamtion troubles - (reply: 2)
  168. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
  169. Problem with Digestion or Ligation. How to tell? - (reply: 7)
  170. Double digest question for ligation - (reply: 4)
  171. Ligation reaction gel picture - (reply: 1)
  172. Cloning/Ligation: How not to have Insert/insert binding? - (reply: 5)
  173. Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
  174. should I go on to the ligation? urgent - (reply: 4)
  175. blunt end ligation problem - (reply: 4)
  176. cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
  177. Ligation ratio problem - (reply: 2)
  178. transformation:more colonies with control! - control plate twice as many colonies as ligation (reply: 8)
  179. Ligation:blunt vs single base overhang - an old question? (reply: 5)
  180. Weird result (ligation) - (reply: 13)
  181. ligation and miniprep - (reply: 6)
  182. two fragment ligation - (reply: 3)
  183. How about 4 DNA fragments ligation and cloning - Cloning by 4 DNA fragments ligation and transformation (reply: 6)
  184. Ligation of half blunt PCR fragments - (reply: 4)
  185. Digestion, Ligation or transformation problem? - (reply: 8)
  186. preciptiate in Fermentas T4 ligase buffer - (reply: 1)
  187. about ligation problem - insert and vector size (reply: 9)
  188. Ligation at 16°C,ON: where to keep it till transformation? - (reply: 4)
  189. Ligation of a 1.8kb insert into 10.8kb vector - (reply: 4)
  190. Ligation into pET28 - (reply: 9)
  191. how to ligate random oligos to vector to construct a library? - (reply: 6)
  192. Is it posible to make ligation with DNA digested and stored at -20 - (reply: 2)
  193. Ligation insert without PCR? - (reply: 6)
  194. How many ligase units? - probably a very stupid question... (reply: 4)
  195. why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
  196. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
  197. volume of ligation product - (reply: 6)
  198. Strange result after ligation and transformation - (reply: 2)
  199. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
  200. very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
  201. Trouble with ligation - (reply: 7)
  202. How long for ligations? - (reply: 11)
  203. Question about phosphorylation and ligation - (reply: 1)
  204. ligation problem - (reply: 6)
  205. blunt end ligation after inverse PCR - (reply: 5)
  206. Ligation problems - (reply: 12)
  207. Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
  208. Ligation failed? - (reply: 3)
  209. 38bp insert and 6.7kb vector ligation problem - the ligation efficiency is too low (reply: 8)
  210. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
  211. Agaroase gel analysis of ligation reaction - (reply: 6)
  212. Help with ligation using Topo TA - (reply: 7)
  213. Clonning problems - my ligation didn't work (reply: 4)
  214. transformation troubles - where to go next? - transformation/ligation (reply: 11)
  215. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  216. new puzzles on my 4-way ligation results - (reply: 6)
  217. ligation and proof reading Taq - (reply: 4)
  218. ligation product- direct pcr - (reply: 2)
  219. is amplification directly from my ligation reaction possible? - (reply: 1)
  220. problems in ligation of pET28b(+) - Ligation (reply: 5)
  221. genomic insert ligation - (reply: 3)
  222. one question on the 4-way ligation - (reply: 10)
  223. a large insert ligation - (reply: 2)
  224. ligation - (reply: 3)
  225. Fuzzy bands on agarose gel after ligation - (reply: 7)
  226. ligation ratio insert-vector - (reply: 6)
  227. ligation: only false positives. Advice needed - (reply: 2)
  228. can I take some of ligation mix and digest? - (reply: 4)
  229. Control PCR in Ligation - Genomic DNA + linker ligation (reply: 1)
  230. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
  231. Multiple ligations - (reply: 3)
  232. Double Blunt Ended Ligation? - (reply: 8)
  233. T4 DNA ligase and RNA - (reply: 2)
  234. Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
  235. one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
  236. Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
  237. ligation never works even for single cut vector!Why so!Help! - (reply: 7)
  238. Block-it Plasmid Ligation - (reply: 8)
  239. Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
  240. Troubleshooting Ligation/Transformation - (reply: 8)
  241. ligase inhibitors - (reply: 5)
  242. VECTOR LIGATION WITH One RE - (reply: 4)
  243. Afl II linearization & ligation - (reply: 3)
  244. using diluted ligase - (reply: 3)
  245. Should I purify digested vector before ligation? - (reply: 7)
  246. Ligation and cloning failure - (reply: 3)
  247. restriction direct to ligation? - skip clean up? (reply: 12)
  248. Linkers:insert ligation problem - (reply: 3)
  249. Ligation and Cloning Problem - Cloing Problem with pBSK (reply: 1)
  250. Freeze a complete ligation? - (reply: 3)
  251. help with ligation and gel extraction - (reply: 5)
  252. Problems with gel extraction and ligation - (reply: 12)
  253. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
  254. more ligation problems! - (reply: 4)
  255. Ligation of incompatible overhangs - (reply: 8)
  256. Ligation or transformation problem - (reply: 12)
  257. complete ligation HELP?! - (reply: 1)
  258. weird blunt-sticky ligation case - (reply: 2)
  259. Ligations with T4 DNA ligase - (reply: 4)
  260. restriction to ligation - (reply: 5)
  261. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  262. Ligation Failure - 20 colonies tested with no results. Need advice (reply: 2)
  263. ligation only concatenate and not circularize - (reply: 2)
  264. Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
  265. how to ligate two ends with different enzyme site - (reply: 3)
  266. Problems about ligation - (reply: 4)
  267. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
  268. ligation - (reply: 1)
  269. is there a restriction about amount of vector in a ligation? - (reply: 14)
  270. Triple ligation with IRES - (reply: 2)
  271. About Ligation :ph34r: - (reply: 1)
  272. blunt-end ligation problem - (reply: 3)
  273. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
  274. Role of PEG in ligation - (reply: 3)
  275. Does the T4 ligase works perfectly well in the presence of TAE and EtBr? - (reply: 1)
  276. Blunt end ligation help - (reply: 2)
  277. What happens to my ligation system? - Very Very urgent! (reply: 3)
  278. No colony after ligation? - (reply: 3)
  279. TA ligation - (reply: 4)
  280. Ligation of multi-fragments? - Has anybody tried to ligate more than 02 fragments in a single experiment? (reply: 2)
  281. Ligation drama/trauma continues - did this work? - (reply: 2)
  282. Ligation over a six day period - WOuld this have damaged the DNA? (reply: 4)
  283. No colony after ligation - (reply: 6)
  284. Ligation worked - maybe? - Aimikins - I've got colonies! Which size do I pick? (reply: 4)
  285. toxic gene ligation/transformation URGENT HELP! - (reply: 1)
  286. how to move for ligation with low conentration of digested plasmid - (reply: 2)
  287. problem in ligation and transformation - (reply: 18)
  288. which quick ligation kit should I use ? - (reply: 1)
  289. Q for ligation ? - (reply: 1)
  290. Is it obligatory to precipitate DNA from ligation before transformed into E.coli - (reply: 7)
  291. ligation problem - (reply: 2)
  292. ligation question! - (reply: 2)
  293. No colonies in my ligation - (reply: 3)
  294. ligation trouble - (reply: 2)
  295. How to sequester SDS in order to do ligation? - (reply: 6)
  296. ligation problem - (reply: 1)
  297. phosphatase removal in ligation - (reply: 3)
  298. temp for ligation - (reply: 2)
  299. DNA purity for ligation - (reply: 1)
  300. I need help --- ligation troubleshooting - (reply: 9)
  301. ligation with sticky and blunt ends - (reply: 3)
  302. ligation inhibitors - (reply: 2)
  303. ligation of two DNA fragments - (reply: 1)
  304. Double-stranded RNA to RNA ligation - (reply: 1)
  305. ligation into pcDNA3.1(-) - (reply: 3)
  306. Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
  307. how to confirm ligation is successful - (reply: 3)
  308. Help on digestion and ligation...colonies not wanted why? - (reply: 4)
  309. problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
  310. Ligation and transformation - (reply: 3)
  311. gel purification necessary for ligation? - (reply: 3)
  312. purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
  313. Is it necessary to inactivate ligase after ligation? - (reply: 2)
  314. Ligation without insert shows colonies - (reply: 1)
  315. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
  316. why my ligation can not work - (reply: 2)
  317. Ligation Problem - Molecular Biology (reply: 2)
  318. Ligation Problems - (reply: 5)
  319. blunting with no success! - blunt end ligation (reply: 1)
  320. can you ligate gel slices directly? - (reply: 8)
  321. gel purification of the vector... - is it good enough for ligation? (reply: 7)
  322. Ligation of big vs small - (reply: 3)
  323. KpnI Ligation Mystery - Bidirectional Cloning Working Unidirectionally (reply: 5)
  324. Do you kill the ligation product? - (reply: 9)
  325. not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
  326. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  327. Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
  328. ligation, transformation problem - (reply: 8)
  329. A basic question about ligation - (reply: 3)
  330. Do I need CIP before my ligation - (reply: 4)
  331. Blunt ended ligation - (reply: 7)
  332. Transformation and ligation problems - (reply: 3)
  333. ligation problems - (reply: 8)
  334. how to confirm if ligation is sucessful? - (reply: 4)
  335. To inactivate or not to inactivate ligase before transformation? - (reply: 5)
  336. Please help me! my ligation is fail - (reply: 6)
  337. How to calculate DNA concentration for ligation - (reply: 8)
  338. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  339. PCR of ligation products - (reply: 8)
  340. over time ligation - (reply: 5)
  341. Troubleshooting ligation/transformation - (reply: 1)
  342. Ligation/Transformation problem - (reply: 4)
  343. Ligation help needed! - vector religation? (reply: 3)
  344. Incubation conditions of T4 DNA ligase - (reply: 4)
  345. how to improve ligation efficiency - (reply: 7)
  346. What is a three-way ligation - (reply: 1)
  347. Ligation is killing me - 1kb insert, 6 kb vector (reply: 11)
  348. strange ligation problem - (reply: 1)
  349. ligation to transformation - (reply: 1)
  350. Ligation help ! - blunt end ligation probs. (reply: 4)
  351. Ligation of large DNA fragments (40 kb + 10 kb) - Does anyone have any suggestions? (reply: 2)
  352. Vector self ligation? - (reply: 1)
  353. Double Ligation - (reply: 8)
  354. ligating small oligos - how to ligate small oligos into vector (reply: 2)
  355. ligation question - (reply: 6)
  356. Ligation problem - (reply: 1)
  357. ligation of short oligos - (reply: 3)
  358. How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
  359. three way ligation (two fragment and a vector) - (reply: 1)
  360. Ligation at 16°C - problems wit ligation (reply: 6)
  361. Fast ligation - (reply: 8)
  362. 4 versus 16 degree for ligation reaction - (reply: 3)
  363. seemingly easy ligations don't work - (reply: 11)
  364. pcr ligation - (reply: 2)
  365. double enzyme cut and ligation - (reply: 3)
  366. Ligation woes - ligation? what's wrong (reply: 2)
  367. problems with same enzyme cutted DNAs ligation - (reply: 1)
  368. Calculate insert/vector ratio for ligation - (reply: 8)
  369. Ligation reaction - temperature and duration consideration - (reply: 5)
  370. ligation/transformation issue - (reply: 5)
  371. pBluescript ligation issues - pBS + thymidine kinase ligation (reply: 3)
  372. Double digestion/ligation problem - (reply: 4)
  373. Help on ligation of big fragment(9kb). - ideas on good ligases? (reply: 2)
  374. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
  375. Double digestion of PCR product and ligation - (reply: 5)
  376. ligation problem - (reply: 3)
  377. Can you store bacteria for ligation? - (reply: 5)
  378. ligation problem of double digested products - (reply: 3)
  379. DNA construction not working--is prep poisoning ligation? - DNA preparation/ligation problems (reply: 5)
  380. help: three fragment ligation - (reply: 1)
  381. Ligation issues - (reply: 1)
  382. ligation problem.. - (reply: 3)
  383. PPT in T4 DNA ligase buffer - (reply: 5)
  384. trouble shooting ligation and transform - (reply: 2)
  385. difficult ligation/transformation - please help (reply: 11)
  386. Has my ligation work? - (reply: 7)
  387. Blunt-Cohesive ends ligation - Blunt-Cohesive ends ligation (reply: 2)
  388. Ligation problems - (reply: 2)
  389. Ligation of two oligos into a vector - (reply: 4)
  390. Is it ok to store a digested vector for ligation? - (reply: 4)
  391. three fragments ligation problems - emergent help needed (reply: 2)
  392. should we dilute the DNA (vector and insert), before ligation? - ligation problem (reply: 5)
  393. too low DNA conc. after ligation..help! - (reply: 1)
  394. ligation problem - (reply: 6)
  395. Ligate two fragments with only one sticky end - (reply: 1)
  396. double restriction digestion - no ligation (reply: 6)
  397. Ligation problems - (reply: 3)
  398. Ligation of 2 fragments and one vector - (reply: 7)
  399. ligation of 2 linear sticky fragments - (reply: 4)
  400. DNA concentration for ligation - (reply: 8)
  401. factors contribute to the ligation failure.. - (reply: 1)
  402. Double insert ligation? - (reply: 2)
  403. ATP in ligation reaction - ligation efficiency (reply: 3)
  404. antibiotics concentration for ligated plasmid - (reply: 3)
  405. Self ligation of vector - (reply: 2)
  406. Store ligation reaction for repeat transformation? - (reply: 3)
  407. Ligation optimization - (reply: 4)
  408. blunt-sticky ligation/transformation problem - (reply: 6)
  409. ligation problem and dephosphorylate matters.... - (reply: 4)
  410. Ligation time - (reply: 7)
  411. help with big DNA ligation - (reply: 6)
  412. Blunt End Ligation !Which god to pray? - (reply: 2)
  413. ligation problem.. - (reply: 3)
  414. problem with ligation with ClaI - Cloning (reply: 7)
  415. ligation problems - ligation problems (reply: 7)
  416. Problem in the ligation - (reply: 2)
  417. Can I PCR amplify a ligation product directly - (reply: 3)
  418. Why ligation reactions are done at 4C - Ligation of insert into vector (reply: 2)
  419. poor ligation of plasmid/oligo construct - (reply: 8)
  420. CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
  421. Ligation of PCR products - (reply: 1)
  422. Cloning, ligation, transformation with pET - Problem re-ligating with NotI (reply: 1)
  423. vector auto ligation problem - (reply: 1)
  424. T4 ligase - is the inactivation step necessary? - (reply: 4)
  425. ligation problem - self ligation of vector - (reply: 5)
  426. Ligate two restriction fragments of dsDNA - (reply: 6)
  427. Recombinant DNA - Problems with ligation (reply: 1)
  428. Unstable plasmid ?? - Deletions only after ligation ! (reply: 2)
  429. Help! Three way ligation including blunt-end oligo - (reply: 2)
  430. The amount of plasmid for ligation reaction - Help (reply: 2)
  431. blunt end ligation problems - (reply: 6)
  432. PCR Ligation cloning - (reply: 2)
  433. Cloning problem - Digestion, ligation and transformation (reply: 6)
  434. ligation question - ligation (reply: 3)
  435. ligation question - ligation of size-selected DNA (reply: 4)
  436. Vector- Insert Ratio in Ligation - (reply: 2)
  437. Tripple Ligation - (reply: 7)
  438. ligation and transformation problems! Urgent! - (reply: 20)
  439. ligation and transformation - (reply: 11)
  440. random ligation question?? - (reply: 1)
  441. Ligation - (reply: 5)
  442. ligation of pcr product - (reply: 4)
  443. ligation problem - (reply: 1)
  444. ligation problems and searh for map of pGW1 vector - (reply: 1)
  445. Why don't get the desired ligation - (reply: 1)
  446. Ligations suck. - (reply: 6)
  447. ligation issues - Ligation (reply: 9)
  448. Help: try ligation but see nothing from the gels - (reply: 9)
  449. ligation help - (reply: 13)
  450. ligation - stick 3 pieces together - (reply: 10)
  451. Impossibly small ligation products - Impossibly small ligation products (reply: 3)
  452. Simple Ligation/cloning questions - question on dNTPs and ligation protocol (reply: 1)
  453. How to ligate ds-oligos into a vector? - (reply: 48)
  454. Problematic ligation - problems with ligation (reply: 1)
  455. Ligation Reaction - (reply: 8)
  456. ligation troubles... - (reply: 2)
  457. Ligation with PLAFR - (reply: 1)
  458. Ligation into EGFP problems - Ligation into EGFP problems (reply: 5)
  459. Double digestion and ligation problem - (reply: 15)
  460. ligation again - (reply: 3)
  461. ligation problem - (reply: 2)
  462. pcr ligation - problems with ligation (reply: 1)
  463. Ligation protocol - (reply: 1)
  464. about ligation and transformation - anxiety (reply: 3)
  465. 42bp fragment ligation to 4776bp vector - (reply: 3)
  466. Stupid Ligation - (reply: 1)
  467. ligase three fragments - ligase three fragments (reply: 3)
  468. Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
  469. 3-fragment ligation - (reply: 3)
  470. Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
  471. RNA  in plasmid preparation interfere in ligation - RNA contamination and ligation (reply: 3)
  472. Any experience in improving blunt end ligation? - (reply: 6)
  473. Help with ligation into IMPACT-CN pTyb11! - (reply: 2)
  474. ligation - (reply: 1)
  475. rapid ligation - (reply: 1)
  476. about rapid ligation - (reply: 2)
  477. Ligation problems - (reply: 6)
  478. Ligation problems - (reply: 4)
  479. Met trouble in ligation! - insert:sacII,XhoI;vector:sacII,SalI (reply: 1)
  480. Failure of ligation - (reply: 1)
  481. ligation problem - (reply: 2)
  482. ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
  483. oligo hybridization and ligation - hybridization and ligation (reply: 1)
  484. Subgenomic library construction - ligation problems - (reply: 2)
  485. rapid ligation - (reply: 3)
  486. DNA ligation - (reply: 3)
  487. ligate 3 DNA fragments with sticky ends into Vector - (reply: 1)
  488. ligation problem with the insert size bigger than 4kb - (reply: 5)
  489. Ligations - (reply: 2)
  490. ligation - the problem of ligation? (reply: 4)
  491. RNA ligation - (reply: 3)
  492. TOPO ligation - (reply: 1)
  493. ligation problem - (reply: 5)
  494. Confused ligation - (reply: 4)