1. What do u think about my protocol for EMSA with antibodies? - EMSA experts out there??? (reply: 1)
2. PMSF precipitate? - (reply: 5)
3. protein concentration measurement - (reply: 4)
4. general protein purification from mouse tissue - (reply: 3)
5. SDS-PAGE sample preparation - (reply: 1)
6. Secondary antibody - (reply: 2)
7. Different intensity present in reference protein even though BCA performed - (reply: 1)
8. How long a membrane can be saved stripping? - (reply: 1)
9. sticked protien - (reply: 2)
10. could you comment on this Sample Preparation Protocol for SDS-PAGE from Muscle t - (reply: 4)
11. High background in western blot - (reply: 2)
12. cell fractionation and low protein concentration in cytosol - (reply: 1)
13. Technique to estimate the level of protein expression from media - (reply: 2)
14. protein concentration of samples in Laemmli buffer - (reply: 1)
15. GST pull down assay - negative control stops working (reply: 2)
16. Protein preicipitated after Dialysis - (reply: 3)
17. Methods of concentrating Protein lysate - (reply: 2)
18. WB vs Immunostaining - (reply: 1)
19. How to normalize ELISA measurements? - Total protein, cell number, or DNA? (reply: 1)
20. 1% boiling buffer for protein extractions - (reply: 3)
21. Volatile Buffers for pH 3 - 10 - Generating a list of volatile buffers for mass spectrometry (reply: 1)
22. efficient substitute of the pulse-chase analysis? - (reply: 2)
23. 2D-electrophoresis - “hollow” effect and streaking problems (reply: 5)
24. Protocol for frozen CPDA blood isolation of haemoglobin - Need help! (reply: 1)
25. loading control - (reply: 1)
26. amino acid homology - %identities vs %positive identities (reply: 2)
27. GST-tagged protein expression problem-plz help - (reply: 3)
28. pROTEIN DOMAINS - new to proteomics (reply: 3)
29. Membrane protein problem - Problem in getting signals (reply: 3)
30. need help - (reply: 1)

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