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Dephosphorylation Related Discussions
  1. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
  2. Dephosphorylation and IP - (reply: 12)
  3. Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
  4. ddephosphorylating proteins using acid phosphatase - (reply: 1)
  5. Do you discard phosphatase before ligation? - (reply: 6)
  6. Protease / Phosphatase Inbibitor necessary ? - (reply: 4)
  7. vector dephosphorylation and ligation - how does it work? (reply: 1)
  8. Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
  9. Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
  10. how to find a phosphatase for a given molecule - (reply: 1)
  11. Phosphatase activity after IP - (reply: 2)
  12. CIP and protein - (reply: 2)
  13. Phosphatase Assay help - (reply: 1)
  14. Phosphatase treatment of sticky ended products? - (reply: 7)
  15. Coupling Antibodies to alkaline phosphatase - (reply: 2)
  16. Diluent buffer for CIP phosphatase, glycerol conc? - (reply: 1)
  17. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
  18. alkaline phosphatase and PBS - (reply: 3)
  19. double digestion and dephosphorylation - (reply: 5)
  20. Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
  21. phosphatase inhibitor treatment - (reply: 1)
  22. CIP - (reply: 3)
  23. alkaline phosphatase (ALP) assay - urgent (reply: 1)
  24. Can CIP dephosphorylate 3' protruding end? - (reply: 3)
  25. ligation problem and dephosphorylate matters.... - (reply: 4)
  26. DNA got degraded after klenow, CIP and Phenol extraction - (reply: 1)
  27. de-phosphorylating a vector - (reply: 7)
  28. Rephosphorylation of SAP treated DNA fragment - (reply: 1)
  29. 3' overhang dephosphorylation? - dephosphorylation problems after KpnI dig (reply: 4)
  30. phosphatase inhibitors - what do I use for with ant-P-Ser IP? (reply: 1)
  31. dephosphorylation - (reply: 1)
  32. CIP problems - (reply: 1)