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Dephosphorylation Related Discussions
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Dephosphorylation of proteins in lysate - need a protocol (reply: 1)
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Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
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Phosphatase treatment as a control for Phospho antibodies - (reply: 1)
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Dephosphorylation and IP - (reply: 12)
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Mammalian Phosphatase inhibitors - (reply: 6)
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Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
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phophorylating PCR product after CIP - (reply: 2)
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ddephosphorylating proteins using acid phosphatase - (reply: 1)
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dephos with alkaline phosphatase - (reply: 2)
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Do you discard phosphatase before ligation? - (reply: 6)
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SAP incubation time - how long do you incubate for?? (reply: 1)
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Protease / Phosphatase Inbibitor necessary ? - (reply: 4)
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cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
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vector dephosphorylation and ligation - how does it work? (reply: 1)
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Overtreatment by dephosphorylating enzymes - (reply: 7)
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Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
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Alkaline Phosphatase and PBS with Magnesium - (reply: 2)
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Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
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Sticky-end dephosphorylation - (reply: 3)
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how to find a phosphatase for a given molecule - (reply: 1)
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Problem in dephosphorylation - (reply: 7)
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Phosphatase activity after IP - (reply: 2)
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Dephosphorylation - (reply: 4)
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CIP and protein - (reply: 2)
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CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
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Phosphatase Assay help - (reply: 1)
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phosphatase inhibitor cocktails... - do you use them or a preffered one? (reply: 4)
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Phosphatase treatment of sticky ended products? - (reply: 7)
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Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
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Coupling Antibodies to alkaline phosphatase - (reply: 2)
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CIP and alkaline phosphatase - what's the difference? (reply: 5)
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Diluent buffer for CIP phosphatase, glycerol conc? - (reply: 1)
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interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
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To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
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Dephosphorylation nick problem - (reply: 2)
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alkaline phosphatase and PBS - (reply: 3)
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phosphatase removal in ligation - (reply: 3)
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double digestion and dephosphorylation - (reply: 5)
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Troubles with vector dephosphorylation - troubles with vector dephosphorylation (reply: 2)
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Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
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Do I need CIP before my ligation - (reply: 4)
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phosphatase inhibitor treatment - (reply: 1)
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CIP with restriction digestion - (reply: 5)
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CIP - (reply: 3)
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protein dephosphorylation by alkaline phosphatase? - (reply: 8)
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alkaline phosphatase (ALP) assay - urgent (reply: 1)
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Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
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Can CIP dephosphorylate 3' protruding end? - (reply: 3)
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phosphatase alkaline before cloning? - (reply: 3)
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ligation problem and dephosphorylate matters.... - (reply: 4)
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Alkaline Phosphatase quantitation - Need help using nitrophenol (reply: 1)
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DNA got degraded after klenow, CIP and Phenol extraction - (reply: 1)
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CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
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de-phosphorylating a vector - (reply: 7)
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Dephosphorylation of vector before ligation - Is it required? (reply: 38)
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Rephosphorylation of SAP treated DNA fragment - (reply: 1)
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serine/threonine phosphatase inhibitors - what are the commonly used ones (reply: 5)
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3' overhang dephosphorylation? - dephosphorylation problems after KpnI dig (reply: 4)
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western blotting using alkaline phosphatase - (reply: 4)
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phosphatase inhibitors - what do I use for with ant-P-Ser IP? (reply: 1)
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3D structure - alkaline phosphatase (reply: 1)
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dephosphorylation - (reply: 1)
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Alkaline phosphatase - (reply: 2)
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CIP problems - (reply: 1)